Abstract
The thermotolerant yeast strain, Kluyveromyces marxianus IMB3, was found to be capable of ethanol production during growth at 45°C on media containing milled paper and exogenously added commercial cellulase. At maximum achievable cellulose concentrations in shake-flask cultures, ethanol production increased to 6.6 g/l at 45°C, representing an overall level of conversion of 21% of the maximum theoretical yield. Subsequent studies involving variations in added cellulase concentrations to the batch systems demonstrated that ethanol yields could be increased to 10 g/l at 45°C, which represented 39% of the maximum theoretical yield. As a result of ethanol production at 45°C in the systems examined, we suggest that the thermotolerant ethanol-producing yeast strain K. marxianus represents a novel candidate for use in simultaneous saccharification and conversion of the resulting substrates to ethanol.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Asenjo JA, Sun W-H, Spencer JL (1991) Optimization of batch processes involving simultaneous enzyme and microbial reactions. Biotechnol Bioeng 37:1087–1094
Banat IM, Nigram P, Marchant R (1992) Isolation of thermotolerant, fermentative yeasts growing at 52°C and producing ethanol at 45°C and 50°C. World J Microbiol Biotechnol 8:259–263
Barron N, Marchant R, McHale L, McHale AP (1994) Growth of a thermotolerant ethanol-producing strain of Kluyveromyces marxianus on celluboise containing media. Biotechnol Lett (in press)
Fleming M, Barron N, McHale L, Marchant R, McHale AP (1993) Studies on the growth of a thermotolerant yeast strain, Kluyveromyces marxianus IMB3, on sucrose-containing media. Biotechnol Lett 15:1195–1198
McHale AP, Morrison J (1986) Cellulase production during growth of Talaromyces emersonii CBS 814.70 on lactose containing media. Enzyme Microb Technol 8:749–754
Philippidis GP, Smith TK, Wyman CE (1993) Study of the enzyme hydrolysis of cellulose for production of fuel ethanol by the simultaneous saccharification and fermentation process. Biotechnol Bioeng 41:846–853
Vallander L, Eriksson EL (1990) Production of ethanol from lignocellulosic materials: state of the art. Adv Biochem Eng 42:63–95
Wright JD, Wyman CE, Grohmann K (1988) Simultaneous saccharification and fermentation of lignocellulose: process evaluation. Appl Biochem Biotechnol 18:75–90
Xin Z, Yimbo Q, Peiji G (1993) Acceleration of ethanol production from paper mill waste fiber by supplementation with β-glucosidase. Enzyme Microb Technol 15:62–65
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Barron, N., Marchant, R., McHale, L. et al. Studies on the use of a thermotolerant strain of Kluyveromyces marxianus in simultaneous saccharification and ethanol formation from cellulose. Appl Microbiol Biotechnol 43, 518–520 (1995). https://doi.org/10.1007/BF00218459
Received:
Revised:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00218459