Summary
Polyclonal antibodies have been raised against purified bovine pyrazine-binding protein, a protein that binds the odorant 2-isobutyl-3-methoxypyrazine. These antibodies have been utilized in immunocytochemical experiments to localize the pyrazine-binding protein in bovine nasal mucosa. Tissue fragments, macroscopically identified as olfactory and respiratory mucosa, were fixed in Bouin's fluid and embedded in paraffin. Consecutive serial sections were processed for immunofluorescence studies and restained either with haematoxylin-eosin or with periodic acid Schiff-Alcian Blue. In both olfactory and respiratory mucosa, only seromucous tubulo-acinar glands were specifically labelled. These glands are located in the lamina propria underlying typical respiratory epithelium, even in those tissues that are macroscopically defined as olfactory mucosa.
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References
Amoore JE (1967) specific anosmia: a Clue to the olfactory code. Nature 214:1095–1098
Bignetti E, Cavaggioni A, Pelosi P, Persaud K, Sorbi RT, Tirindelli R (1984) Purification and characterization of an odorantbinding protein from cow nasal tissue. VI∘ E.C.R.O. Congress, Lyon-Ecully
Bignetti E, Cavaggioni A, Pelosi P, Persaud K, Sorbi RT, Tirindelli R (1985) Purification and characterization of an odorant-binding protein from cow nasal tissue. Eur J Biochem 149:227–231
Coons AH, Leduc EH, Connolly JM (1955) Study of antibody production. I. A method for the histochemical demonstration of specific antibody and its application to a study of the hyperimmune rabbit. J Exp Med 102:49–60
Cuschieri A, Bannister LH (1974) Some histochemical observations on the mucosubstances of the nasal glands of the mouse. Histochem J 6:543–558
Fairbanks G, Steck TE, Wallach DFH (1971) Electrophoretic analysis of the major polypeptides of the human erythrocyte membrane. Biochemistry 10:2606–2616
Fesenko EE, Novoselov VI, Krapivinskaja LD (1979) Molecular mechanism of olfactory reception. IV. Some biochemical characteristics of the camphor receptor from rat olfactory epithelium. Biochim Biophys Acta 587:424–433
Getchell TV, Zielinski B, Getchell ML (1985) Pyrazinemediated neural and secretory activity in the olfactory mucosa of the salamander. A ChemS VII Absts 67
Graziadei PPC (1971) The olfactory mucosa of vertebrates. In: Beidler EM (ed) Handbook of Sensory Physiology. Chemical Senses, 1: Olfaction. Springer, Berlin Heidelberg New York, Vol. 4, cpt 2
Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680–685
Mowry RW (1956) Alcian blue technics for the histochemical study of acidic carbohydrates. J Histochem Cytochem 4:407
Pelosi P, Baldaccini NE, Pisanelli AM (1982) Identification of a specific olfactory receptor for 2-isobutyl-3-methoxypyrazine. Biochem J 201:245–248
Pelosi P, Pisanelli AM, Baldaccini NE, Gagliardo A (1981) Binding of 3H-2-isobutyl-3-methoxypyrazine to cow olfactory mucosa. Chem Senses 6:77–85
Persaud K, Dodd G (1981) Biochemical mechanism in vertebrate primary olfactory neurons. In: Cagan RH, Kare MR (eds) Biochemistry of Taste and Olfaction. Academic Press, New York, pp 333–357
Pevsner J, Trifiletti RR, Strittmatter SM, Snyder SH (1985) Isolation and characterization of an olfactory receptor protein for odorant pyrazines. Proc Natl Acad Sci USA 82:3050–3054
Sever JE (1962) Application of a microtechnique to viral serological investigations. J Immunol 88:320
Towbin H, Staehelin T, Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc Natl Acad Sci USA 76:4350–4354
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Avanzini, F., Bignetti, E., Bordi, C. et al. Immunocytochemical localization of pyrazine-binding protein in bovine nasal mucosa. Cell Tissue Res. 247, 461–464 (1987). https://doi.org/10.1007/BF00218329
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DOI: https://doi.org/10.1007/BF00218329