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Immunocytochemical localization of insulin and somatostatin in the endocrine pancreas of the sea lamprey Petromyzon marinus L., at various stages of its life cycle

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Summary

Light-microscopic immunocytochemistry and routine staining techniques were used to localize insulin and somatostatin-immunoreactive cells within the endocrine pancreatic tissue of the lamprey, Petromyzon marinus, during various stages of the life cycle. The endocrine pancreas of larvae consists solely of follicles of insulin-immunoreactive cells surrounding the junction of oesophagus, intestine and bile duct. Somatostatin-immunoreactive cells are restricted to the intestinal epithelium. In both parasitic and upstream-migrating adults the endocrine pancreas consists of cranial and caudal portions, both containing separate populations of insulin and somatostatin-immunoreactive cells.

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References

  • Barrington EJW (1972) The pancreas and intestine. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol. 2. Academic Press, London, p. 135–169

    Google Scholar 

  • Boenig H (1927) Studien zur Morphologie und Entwicklungsgeschichte des Pankreas beim Bachneunauge (Lampetra (Petromyzon) planerf). I. Teil. Z mikrosk-anat Forsch 8:489–511

    Google Scholar 

  • Brinn JE, Epple A (1976) New types of islet cells in a cyclostome, Petromyzon marinus L. Cell Tissue Res 171:317–329

    Google Scholar 

  • Clark A, Grant AM (1983) Quantitative morphology of endocrine cells in human fetal pancreas. Diabetalogia 25:31–35

    Google Scholar 

  • Efendić S, Uvnas-Wallensten K (1983) Somatostatin: physiological significance with emphasis on the pancreas and gastrointestinal tract. Period Biol 85 (suppl 1):95–106

    Google Scholar 

  • Epple A (1967) A staining sequence for A-, B-, and D-cells of pancreatic islets. Stain Technol 42:53–61

    Google Scholar 

  • Epple A, Brinn JE (1975) Islet histophysiology: evolutionary correlations. Gen Comp Endocrinol 27:320–349

    Google Scholar 

  • Epple A, Brinn JE, Young JB (1980) Evolution of pancreatic islet functions. In: Pang PKT, Epple A (eds) Evolution of vertebrate endocrine systems. Texas Tech Univ Press, Lubbock, p 269–321

    Google Scholar 

  • Falkmer S, Cutfield JF, Cutfield SM, Dodson GG, Gliemann J, Gammeltoft SG, Marques M, Peterson JD, Steiner DF, Sundby F, Emdin SO, Havu N, Ostberg Y, Winbladh L (1975) Comparative endocrinology of insulin and glucagon production. Am Zool 15 (suppl 1):255–270

    Google Scholar 

  • Falkmer S, Ostberg Y, Van Noorden S (1978) Entero-insular endocrine systems of cyclostomes: a clue to hormone evolution. In: Bloom SR (ed) Gut hormones. Churchill Livingstone, London, p 57–63

    Google Scholar 

  • Hardisty MW (1982) Lampreys and hagfishes: An analysis of cyclostome relationships. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol. 4b. Academic Press, London, p 165–259

    Google Scholar 

  • Hardisty MW, Baker BI (1982) Endocrinology of lampreys. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol. 4b. Academic Press, London, p 1–115

    Google Scholar 

  • King JA, Millar RP (1979) Phylogenetic and anatomical distribution of somatostatin in vertebrates. Endocrinology 105:1322–1329

    Google Scholar 

  • Lillie RD (1951) Simplification of the manufacture of Schiff reagent for use in histochemical procedures. Stain Technol 26:163–165

    Google Scholar 

  • McGadey J (1979) A staining sequence for the differentiation of A-, B-, and D-cells of the islet of guinea pig pancreas. Acta Diabet Latina 16:243–246

    Google Scholar 

  • Stewart JK, Goodner CJ, Koerker DJ, Gorbman A, Ensinck J, Kaufman M (1978) Evidence for a biological role of somatostatin in the Pacific hagfish, Eptatretus stouti. Gen Comp Endocrinol 36:408–414

    Google Scholar 

  • Vandesande F, Dierickx K (1975) Identification of the vasopressin producing and of the oxytocin producing neurons in the hypothalamic magnocellular neurosecretory system of the rat. Cell Tissue Res 164:153–162

    Google Scholar 

  • Van Noorden S, Falkmer S (1980) Gut-islet endocrinology — some evolutionary aspects. Invest Cell Pathol 3:21–35

    Google Scholar 

  • Van Noorden S, Pearse AGE (1974) Immunoreactive polypeptide hormones in the pancreas and gut of the lamprey. Gen Comp Endocrinol 23:311–324

    Google Scholar 

  • Van Noorden S, Polak JM (1979) Hormones of the alimentary tract. In: Barrington EJW (ed) Hormones and evolution, vol. 2. Academic Press, London, p 791–828

    Google Scholar 

  • Van Noorden S, Greenberg J, Pearse AGE (1972) Cytochemical and immunofluorescence investigations on polypeptide hormone localization in the pancreas and gut of the larval lamprey. Gen Comp Endocrinol 19:192–199

    Google Scholar 

  • Van Noorden S, Ostberg Y, Pearse AGE (1977) Localization of somatostatin-like immunoreactivity in the pancreatic islets of the hagfish, Myxine glutinosa and the lamprey, Lampetrafluviatilis. Cell Tissue Res 177:281–285

    Google Scholar 

  • Van Noorden S, Polak M, Pearse AGE (1980) Evolution of the gastrointestinal endocrine system. In: Pang PKT, Epple A (eds) Evolution of vertebrate endocrine systems. Texas Tech Univ Press, Lubbock, p 247–267

    Google Scholar 

  • Wagner GF, McKeown BA (1981) Immunocytochemical localization of hormone-producing cells within the pancreatic islets of the rainbow trout (Salmo gairdneri). Cell Tissue Res 221:181–192

    Google Scholar 

  • Wright GM, Filosa MF, Youson JH (1978) Light and electron microscopic immunocytochemical localization of thyroglobulin in the thyroid gland of the anadromous sea lamprey, Petromyzon marinus L., during its upstream migration. Cell Tissue Res 187:473–478

    Google Scholar 

  • Youson JH (1980) Morphology and physiology of lamprey metamorphosis. Can J Fish Aquat Sci 37:1687–1710

    Google Scholar 

  • Youson JH (1981a) The alimentary canal. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol 3. Academic Press, London, p 95–189

    Google Scholar 

  • Youson JH (1981b) The liver. In: Hardisty MW, Potter IC (eds) The biology of lampreys, vol. 3. Academic Press, London, p 263–332

    Google Scholar 

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Supported by NSERC of Canada grant no. A5945 to JHY

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Elliott, W.M., Youson, J.H. Immunocytochemical localization of insulin and somatostatin in the endocrine pancreas of the sea lamprey Petromyzon marinus L., at various stages of its life cycle. Cell Tissue Res. 243, 629–634 (1986). https://doi.org/10.1007/BF00218071

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