Summary
Quantitative studies on the population of the gonadotropic cells in the pituitary of adult male rats were performed after trypsic dissociation of the pituitary glands and immunoenzymatic staining with anti-β-LH or anti-β-FSH antisera. Number, area and extinction of labelled cells were measured by use of an image analyser and a cytophotometer. The gonadotrophs represent approximately 14% of the pituitary cells. The mean area of gonadotrophs is significantly larger after staining with anti-β-LH serum than after staining with anti-β-FSH serum. Planimetric measurement of the gonadotrophs reveals their variability in size ranging between 30 and 160 μm2. Moreover, the size distribution depends on the staining serum used: more numerous small-sized cells (<75 μm2) are stained with anti-β-FSH serum than with anti-β-LH serum, which conversely stains more numerous large-sized cells. Cytophotometric measurements indicate that immunostaining varies greatly among cells of the same size class and that the staining intensity appears to increase according to the cell size. These results emphasize the morphofunctional heterogeneity of the gonadotropic cell population.
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References
Batten TFC, Hopkins CR (1978) Discrimination of LH, FSH, TSH and ACTH in dissociated porcine anterior pituitary cells by light and electron microscope immunocytochemistry. Cell Tissue Res 192:107–120
Bugnon C, Fellmann D, Lenys D, Bloch B (1977) Etude cytoimmunologique des cellules gonadotropes et des cellules thyréotropes de l'adénohypophyse du Rat. C R Soc Biol 171:907–913
Bugnon C, Fellmann D, Bresson JL (1980) Etude planimétrique et absorptiométrique des cellules gonadotropes isolées de l'adénohypophyse du rat mâle adulte. Réunion de la Société Française de Neuroendocrinologie Expérimentale. Montpellier, Abstract in J Physiol (Paris) in press
Dacheux F (1980) Ultrastructural localization of LH and FSH in the porcine pituitary. In: Jutisz M, McKerns W (eds) Synthesis and release of adenohypophyseal hormones 187–195
Denef C, Hautekeete E, Rubin L (1976) A specific population of gonadotrophs purified from immature female rat pituitary. Science 194:848–851
Denef C, Hautekeete E, De Wolf A, Vanderschueren B (1978a) Pituitary basophils from immature male and female rats: Distribution of gonadotrophs and thyrotrophs as studied by unit gravity sedimentation. Endocrinology 103:724–735
Denef C, Hautekeete D, Dewals R (1978b) Monolayer cultures of gonadotrophs separated by velocity sedimentation: Heterogeneity in response to luteinizing hormone-releasing hormone. Endocrinology 103:736–747
Denef C, Hautekeete E, Dewals R, De Wolf A (1980) Differential control of luteinizing hormone and follicle-stimulating hormone secretion by androgens in rat pituitary cells in culture: Functional diversity of subpopulations separated by unit gravity sedimentation. Endocrinology 106:724–729
El Etreby ME, Fath El Bab MR (1977) Localization of gonadotrophic hormones in the dog pituitary gland. Cell Tissue Res 183:167–175
Hopkins CR (1977) Short term kinetics of luteinizing hormone secretion studied in dissociated pituitary cells attached to manipulable substrates. J Cell Biol 73:685–695
Hopkins CR, Farquhar MG (1973) Hormone secretion by cells dissociated from rat anterior pituitaries. J Cell Biol 59:276–303
Hymer WC, Kraicer J, Bencosme SA, Haskill JS (1972) Separation of somatotrophs from the rat adenohypophysis by velocity and density gradient centrifugation. Proc Soc Exp Biol Med 141:966–973
Malamed S, Portanova R, Sayers G (1971) Fine structure of trypsin-dissociated cells of the rat anterior pituitary gland. Proc Soc Exp Biol Med 138:920–926
Moriarty GC (1976) Immunocytochemistry of the pituitary glycoprotein hormones. J Histochem Cytochem 24:846–863
Nakane PK (1970) Classifications of anterior pituitary cell types with immunoenzyme histochemistry. J Histochem Cytochem 18:9–20
Nakano H, Fawcett CP, McCann SM (1976) Enzymatic dissociation and short-term culture of isolated rat anterior pituitary cells for studies on the control of hormone secretion. Endocrinology 98:278–288
O'Conner JL, Allen MB, Mahesh VB (1980) Castration effects on the response of rat pituitary cells to luteinizing hormone-releasing hormone: Retention is dispersed cell culture. Endocrinology 106:1706–1714
Surks MI, DeFesi CR (1977) Determination of the cell number of each cell type in the anterior pituitary of euthyroid and hypothyroid rats. Endocrinology 101:946–958
Vale W, Grant G, Amoss M, Blackwell R, Guillemin R (1972) Culture of enzymatically dispersed anterior pituitary cells: Functional validation of a method. Endocrinology 91:562–572
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Fellmann, D., Bresson, J.L., Clavequin, M.C. et al. Quantitative immunocytochemical studies on the gonadotrophs isolated from the pituitary of the male rat. Cell Tissue Res. 224, 137–144 (1982). https://doi.org/10.1007/BF00217273
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DOI: https://doi.org/10.1007/BF00217273