Abstract
To measure transcript levels for individual members of the Cab (chlorophyll a/b protein) multigene family in pea under a range of developmental situations, we developed a system using cDNA synthesis, the polymerase chain reaction (PCR), and chemiluminescence detection. In order to design gene-specific PCR primers for all genes, a partial genomic clone for a fifth, Type I LHCII (light-harvesting complex of photosystem II) gene, Cab-9 Footnote 1, was isolated and sequenced. All seven known Cab genes in pea are expressed in light-grown buds and leaves, including several genes previously known only from genomic clones. There appear to be at least two groups of Cab genes in pea which differ in their response to light and development. The first group (consisting of Cab-8, AB96, Cab-215 and Cab-315) includes Type I, Type II and Type III genes, shows a relatively strong response to red light, and has bud transcript levels similar to or slightly higher than leaves. The second group, consisting of the Type I genes Cab-9, AB80 and AB66, shows little or no transcript accumulation 24 h after a red light pulse, and has higher transcript levels in leaves than in buds. Transcript levels for genes in this second group appear to be lower than those of the first group in all developmental situations examined. These data indicate that there has been an evolutionary divergence of the responses to light and development among the Type I LHCII genes.
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Notes
The Cab-9 sequence appears in the Genbank/EMBL databases under the accession number M86906
Abbreviations
- Cab :
-
gene for chlorophyll a/b binding protein
- LHCI, LHCII:
-
light-harvesting complexes of photosystems I and II
- PCR:
-
polymerase chain reaction
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We especially thank Dr. Nam-Hai Chua for the cDNA clone AB96 and Dr. Anthony R. Cashmore for the AB80 and AB66 genomic clones. Michael Murray and Jerry Slightom (Department of Agrigenetics, University of Wisconsin, Madison, USA) generously provided a genomic library of pea DNA from which Cab-9 was isolated. Keith Everett's photography and assistance with the densitometry is gratefully acknowledged. Finally, we thank our laboratory colleagues for their technical expertise and valuable discussions. This work was supported by grants from the National Science Foundation (to W.F.T.) and the National Institutes of Health (to B.A.R.), and in part by a North Carolina Biotechnology fellowship to B.W.F. and an Izaak Walton Killam postdoctoral fellowship to M.J.W. D.F. was supported by the French Ministere Des Affaires Etrangeres.
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White, M.J., Fristensky, B.W., Falconet, D. et al. Expression of the chlorophyll-a/b-protein multigene family in pea (Pisum sativum L.). Planta 188, 190–198 (1992). https://doi.org/10.1007/BF00216813
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DOI: https://doi.org/10.1007/BF00216813