Summary
The effect of histamine on cAMP and cGMP levels in day 6 (144 h post coitum) rabbit blastocysts was determined. Histamine at 200 μM and 1000 μM concentrations stimulated the increased formation of cAMP in vitro, whereas stimulation of cGMP occurred only in the presence of 1000 μM histamine. Furthermore, intrauterine injection of RMI-12330A (50 μg or 500 μg/uterine horn), an inhibitor of adenylyl cyclase, on day 5 of pregnancy interrupted embryro development and implantation of the embryo. The drug was also effective in reducing the cAMP level in the endometrial cells in vitro. A relationship between histamine and cyclic nucleotide changes in embryo development and implantation is suggested.
Similar content being viewed by others
References
Castro-Vasquez A, Gomez E, DeCarli DN, Rosner JM (1976) Further evidence for histamine facilitating oestrogen action in the uterus. J Endocrinol 68:121–126
Clark MR, Marsh JM, LeMaire WJ (1978) Stimulation of prostaglandin accumulation in preovulatory rat follicles by adenosine 3′,5′-monophosphate. Endocrinology 102:39–44
Dey SK, Johnson DC (1980a) Reevaluation of histamine in implantation. In: Kimble F (ed) The endometrium. Sp Medical and Scientific Books, Spectrum Publications, New York, pp 269–283
Dey SK, Johnson DC (1980b) Histamine formation by mouse preimplantation embryos. J Reprod Fert 60:457–460
Dey SK, Kimura F, Mukherjee A, Dickmann Z (1978) Cyclic AMP and cyclic GMP in rabbit blastocysts. J Reprod Fert 52:235–237
Dey SK, Johnson DC, Santos JG (1979a) Is histamine production by the blastocyst required for implantation in rabbit? Biol Reprod 21:1169–1173
Dey SK, Villanueva C, Abdou NI (1979b) Histamine receptors on rabbit blastocyst and endometrial cell membranes. Nature 278:648–649
Dey SK, Chien SM, Cox, CL, Crist RD (1980) Prostaglandin synthesis in the rabbit-blastocyst. Prostaglandins 19:449–453
Flandroy L, Galand P (1978) Changes in cGMP and cAMP content in the estrogen-stimulated rat uterus: temporal relationship with other parameters of hormonal stimulation. J Cyclic Nucleotide Res 4:145–158
Frandsen EK, Kishna G (1976) A simple ultrasensitive method for the assay of cyclic AMP and cyclic GMP in tissues. Life Sciences 18:529–541
Holmes PV, Gordashko BJ (1980) Evidence of prostaglandin involvement in blastocyst implantation. J Embryol Exp Morphol 55:109–122
Kennedy TG (1977) Evidence for a role for prostaglandins in the initiation of blastocyst implantation in the rat. Biol Reprod 16:286–291
Mukherjee A, Dey SK, Sengupta J, Ramadoss CS, Dickmann Z (1978) Regulatory enzymes of carbohydrate and energy metabolism in the rabbit blastocyst. J Reprod Fert 53:77–80
Onaya T, Hashizume K, Sato A, Takazawa K, Akasu F, Endo W (1977) Evidence for the existance of a histamine H2-receptor in the mouse thyroid. Endocrinology 100:61–66
Psychoyos S (1978) H1- and H2-Histamine receptors linked to adenylate cyclase in cell-free preparations of guinea pig cerebral cortex. Life Sciences 23:2155–2162
Reinhardt D, Ritter E, Butzheinen R, Schumann HJ (1979) Relationship between histamine-induced changes of cyclic AMP and mechanical activity on smooth muscle preparations of the guinea pig ileum and the rabbit mesentric artery. Agents and Actions 9:155–162
Siegel BW, Wiech NL (1976) An inhibitor of cholera toxin induced intestinal hypersecretion which also inhibits adenylate cyclase activity. Gastroenterology 70:A-791–937
Steiner AL, Parker CW, Kipnis DM (1972) Radioimmunoassay for cyclic nucleotides. J Biol Chem 247:1106–1113
Study RE, Greengard P (1978) Regulation by histamine of cyclic nucleotide levels in sympathetic ganglia. J Pharmacol Exp Ther 207:767–778
Taylor JE, Richelson E (1979) Desensitization of histamine H1 receptor-mediated cyclic GMP formation in mouse neuroblastoma cells. Mol Pharmacol 15:462–471
Zor U, Strulovici B, Lindner HR (1977) Stimulation by cyclic GMP of prostaglandin E production in isolated ganglion follicles. Biochem Biophys Res Commun 76:1086–1091
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dey, S.K., Hubbard, C.J. Role of histamine and cyclic nucleotides in implantation in the rabbit. Cell Tissue Res. 220, 549–554 (1981). https://doi.org/10.1007/BF00216758
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00216758