Abstract
To better define the regulation and expression of bovine major histocompatibility complex (MHC) antigens, the bovine B lymphoblastoid cell line, BL3, was exposed to γ-irradiation and surviving cells were immunoselected for MHC class I antigen loss. The resulting class I expression loss variant, BL3.1, was characterized at both the protein and genetic levels to ascertain the nature of the defect. Microfluorimetry analysis revealed a 3–5-fold surface density reduction of all class I products on BL3.1 cells relative to the parental BL3 cells. This decreased surface expression was specific for MHC class I and not MHC class II or the non-MHC-linked gene product, immunoglobulin (Ig). Northern and quantitative slot blot analyses demostrated a corresponding diminution of class I RNA in BL3.1 suggesting a transcriptional level defect. Nuclear run-off and transcription inhibition experiments confirmed no post-transcriptional changes while Southern blot analysis provided no evidence for alterations within or near the class I genes. To help elucide the mechanism of altered class I expression, the parent, BL3, and variant, BL3.1, were cultured with factors known to enhance MHC class I transcription. Interferon (IFN)-γ, lipopolysaccharide (LPS), and activated peripheral cell lines induced MHC class I transcription and surface expression 2–3-fold greater than the untreated controls. It is likely, therefore, that a genetic alteration outside of the class I genes has occured within BL3.1 impairing expression of MHC class I.
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Correspondence and offprint requests: G. A. Splitter.
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Harms, J.S., Splitter, G.A. Impairement of MHC class I transcription in a mutant bovine B cell line. Immunogenetics 35, 1–8 (1992). https://doi.org/10.1007/BF00216620
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DOI: https://doi.org/10.1007/BF00216620