Summary
An aminopeptidase N has been detected by immunofluorescence in the apical plasma membrane of porcine thyroid cells, facing the follicular lumen. Freshly isolated cells obtained by tissue trypsinization, lose their polarity and exhibit a homogeneous enzyme distribution over the whole plasma membrane. In thyrotropin-stimulated cultured cells organized into follicles, the enzyme is localized in the apical cell pole. In monolayer cells, on the other hand, the enzyme is distributed over the whole surface facing the medium. In both types of cultures fluorescence is also observed in intracytoplasmic organelles. In vivo, aminopeptidase is a marker of the apical part of the thyroid plasma membrane, but its in vitro localization depends upon cell differentiation related to the culture conditions.
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References
Benajiba A, Maroux S (1980) Purification and characterization of an aminopeptidase A from hog intestinal brush border membrane. Eur J Biochem 107:381–388
Bouchilloux S (1979) Purification by affinity chromatography and some properties of microsomal galactosyltransferase from pig thyroid. Biochim Biophys Acta 569:135–144
Cau P, Michel-Béchet M, Fayet G (1976) Morphogenesis of thyroid follicles in vitro. Adv Anat Embryol Cell Biol 52/2:1–66
Colas B, Maroux S (1980) Simultaneous isolation of brush border and basolateral membrane from rabbit enterocytes. Biochim Biophys Acta 600:406–420
Dickson JG, Hovsépian S, Fayet G, Lissitzky S (1981) Follicle formation and iodide metabolism in cultures of human thyroid cells. J Endocrinol 90:113–124
Eagle H (1959) Amino acid metabolism in mammalian cell cultures. Science 130:432–437
Evans VJ, Bryant JC, Kerr HA, Schilling EL (1964) Chemically defined media for cultivation of longterm cell strains from four mammalian species. Exp Cell Res 36:439–474
Fayet G, Hovsépian S (1979) Demonstration of growth in porcine thyroid cell culture. Biochimie 61:923–930
Fayet G, Hovsépian S (1980) In vitro conversion of porcine thyroid cells growing in monolayer into functional follicular cells. Biochimie 62:27–32
Fayet G, Tixier R (1967) Réassociation in vitro de cellules isolées de thyroide de porc adulte et biosynthèse d'une protéine apparentée à la thyroglobuline. C R Acad Sc D Paris 265:1554–1556
Fayet G, Pachéco H, Tixier R (1970) Sur la réassociation in vitro des cellules isolées de thyroide de porc et la synthèse de la thyroglobuline. I. Conditions pour l'induction des réassociations cellulaires par la thyréostimuline. Bull Soc Chim Biol 52:299–306
Fayet G., Michel-Béchet M, Lissitzky S (1971) Thyrotrophin-induced aggregation and reorganization into follicles of isolated porcine-thyroid cells in culture. II. Ultrastructural studies. Eur J Biochem 24:100–111
Feracci H, Maroux S (1980) Rabbit intestinal aminopeptidase N. Purification and molecular properties. Biochim Biophys Acta 599:448–463
Feracci H, Bernadac A, Hovsépian S, Fayet G, Maroux S (1981) L'aminopeptidase N, un marqueur du pole apical dans l'épithelium thyroidien de porc et dans les cellules en culture. C R Acad Sci (D) Paris 292:271–275
Fujita M (1963) Electron microscopic studies on the thyroid gland of domestic fowl, with special reference to the mode of secretion and the occurence of a central flagellum in the follicular cell. Z Zellforsch 60:615–632
Fujita M, Ohta H, Kawai K, Matsui H, Nakao M (1972) Differential isolation of microvillous and basolateral plasma membrane from intestinal mucosa: Mutually exclusive distribution of digestive enzymes and ouabain-sensitive ATPase. Biochim Biophys Acta 274:336–347
Harber DS, Stern BK, Reilly RW (1964) Removal and dissociation of epithelial cells from the rodent gastrointestinal tract. Nature 203:319–320
Hovsépian S (1980) Multiplication de cellules thyroidiennes de porc en culture, conversion de cellules monocouches en cellules folliculaires fonctionnelles. Theèse de Doctorat d'Université Mention Sciences. Aix-Marseille II, France
Louvard D (1980) Apical membrane aminopeptidase appears at site of cell-cell contact in cultured kidney epithelial cells. Proc Natl Acad Sci USA 77:4132–4136
Louvard D, Maroux S, Baratti J, Desnuelle P, Mutafschiev S (1973) On preparation and some properties of closed membrane vesicles from hog duodenal and jejunal brush-border. Biochim Biophys Acta 291:747–763
Louvard D, Maroux S, Vannier Ch, Desnuelle P (1975 a) Topological studies of the hydrolases bound to the intestinal brush border membrane.I. Solubilization by papain and triton X100. Biochim Biophys Acta 375:236–248
Louvard D, Maroux S, Desnuelle P (1975b) Topological studies on the hydrolases bound to the intestinal brush border membrane. II. Interaction of free and bound aminopeptidase with a specific antibody. Biochim Biophys Acta 389:389–400
Maroux S, Louvard D, Baratti J (1973) The aminopeptidase from hog intestinal brush-border. Biochim Biophys Acta 321:282–295
Miller D, Crane RK (1961) The digestive function of the epithelium of the small intestine. II Localization of disaccharide hydrolysis in the isolated brush border portion of intestinal epithelial cells. Biochim Biophys Acta 52:293–298
Monis B, Wasserkrug H, Seligman AM (1965) Comparison of fixatives and substrates for aminopeptidase. J Histochem Cytochem 13:503–509
Neve P, Rodesch FR, Dumont JE (1968) Electron microscopy of isolated sheep thyroid cells. Exp Cell Res 51:68–78
Remy L, Michel-Béchet M, Cataldo C, Bottini J, Hovsépian S, Fayet G. (1977) The role of intracellular lumina in thyroid cells for follicle morphogenesis in vitro. J Ultrastruct Res 61:243–253
Tixier-Vidal A, Picart R, Rappaport L, Nunez J (1969) Ultrastructure et autoradiographie de cellules thyroidiennes isolées, incubées en présence d' 125I. J Ultrastruct Res 28:78–101
Tokuyasu KT (1973) A technique for ultracryotomy of cell suspension and tissues. J Cell Biol 57:551–565
Tong W, Kerkof P, Chaikoff IL (1962) Iodide metabolism of dispersed thyroid cells obtained by trypsinization of sheep thyroid glands. Biochim Biophys Acta 60:1–19
Vannier Ch, Louvard D, Maroux S, Desnuelle P (1976) Structural and topological homology between porcine intestinal and renal brush border aminopeptidase. Biochim Biophys Acta 455:185–199
Wachsmuth ED, Fritze I, Pfleiderer G (1966) An aminopeptidase occurring in pig kidney. I. An improved method of preparation. Physical and enzyme properties. Biochemistry 5:169–174
Ziomek CA, Schulman S, Edidin M (1980) Redistribution of membrane proteins in isolated mouse intestinal epithelial cells. J Cell Biol 86:849–857
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Feracci, H., Bernadac, A., Hovsépian, S. et al. Aminopeptidase N is a marker for the apical pole of porcine thyroid epithelial cells in vivo and in culture. Cell Tissue Res. 221, 137–146 (1981). https://doi.org/10.1007/BF00216576
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DOI: https://doi.org/10.1007/BF00216576