Summary
An immunohistochemical study of atrial natriuretic polypeptides was carried out on embryonic, fetal and neonatal rat hearts, using an antiserum raised against α-human atrial natriuretic polypeptide (α-hANP). Weakly immunoreactive cells were seen in both atrial and ventricular walls at 11 days post coitum (pc). After this stage, the immunoreactive cells became more intensely stained in both atrial and ventricular walls. The immunoreactivity during the prenatal period was stronger in the superficial cell layer beneath the endocardium, than in the deep cell layer of the atrial wall. The cells in the trabecular meshwork also had an apparent, but weak, immunoreactivity, which showed a greater intensity in the left ventricle than in the right one. It is suggested that these immunoreactive cells in the ventricle may differentiate, in situ, into the cells of the impulse-conducting system during the further development of the heart.
Similar content being viewed by others
References
Bencosme SA, Berger JM (1971) Specific granules in mammalian and non-mammalian vertebrate cardiocytes. In: Bajusz E, Jasmin G (eds) Functional morphology of the heart. Methods Achiev Exp Pathol Vol 5 Karger, Basel, pp 173–213
Berger JM, Rona G (1971) Functional and fine structural heterogeneity of atrial cardiocytes. In: Bajusz E, Jasmin G (eds) Functional morphology of the heart. Methods Achiev Exp Pathol Vol 5. Karger, Basel, pp 540–590
Bold de AJ (1978) Morphometric assessment of granulation in rat atrial cardiocytes: Effect of age. J Mol Cell Cardiol 10:717–724
Bold de AJ (1979) Heart atria granularity effects of changes in water-electrolyte balance. Proc Soc Exp Biol Med 161:508–511
Bold de AJ (1982a) Atrial natriuretic factor of the rat heart studies on isolation and properties. Proc Soc Exp Biol Med 170:133–138
Bold de AJ (1982b) Tissue fractionation studies on the relationship between an atrial natriuretic factor and specific atrial granules. Can J Physiol Pharmacol 60:324–330
Bold de AJ, Borenstein HB, Veress AT, Sonnenberg H (1981) A rapid and potent natriuretic response to intravenous injection of atrial myocardial extract in rats. Life Sci 28:89–94
Bompiani Par GD, Rouilier Ch, Hatt PY (1959) Le tissu de conduction du coeur chez le rat. Etude au microscope électronique. Arch Mal Coeur 52:1257–1274
Cantin M, Gutkowska J, Thibault G, Milne RW, Ledoux S, MinLi S, Chapeau C, Garcia R, Hamet P, Genest J (1984) Immunocytochemical localization of atrial natriuretic factor in the heart and salivary glands. Histochemistry 80:113–127
Challice CE, Virágh Sz (1973) The embryonic development of the mammalian heart. In: Challice CE, Virágh Sz (eds) Ultrastructure of the mammalian heart. Academic Press, New York, pp 91–126
Field EJ (1949) The development of the conducting system in the heart of sheep. Br Heart J 13:129–149
Forsgren S, Thornell L-E (1981) The development of Purkinje fibres and ordinary myocytes in the bovine fetal heart. Anat Embryol 162:127–136
Forsgren S, Carlsson E, Strehler E, Thornell L-E (1982a) Ultrastructural identification of human fetal Purkinje fibres — a comparative immunocytochemical and electron microscopic study of composition and structure of myofibrillar M-regions. J Mol Cell Cardiol 14:437–449
Forsgren S, Eriksson A, Kjöreli U, Thornell L-E (1982b) The conduction system in the human heart at midgestation — Immunohistochemical demonstration of the intermediate filament protein skeletin. Histochemistry 75:43–52
Forsgren S, Strehler E, Thornell L-E (1982c) Differentiation of Purkinje fibres and ordinary ventricular and atrial myocytes in the bovine heart: an immuno- and enzyme histochemical study. Histochcm J 14:929–942
Forssmann WG, Hock D, Lottspeich F, Henschen A, Kreye V, Christmann M, Reinecke M, Metz J, Carlquist M, Mutt V (1983) The right auricle of heart is an endocrine organ. Anat Embryol 168:307–313
Forssmann WG, Birr C, Carlquist M, Christmann M, Finke R, Henschen A, Hock D, Kirchheim H, Kreye V, Lotspeich F, Metz J, Mutt V, Reineck M (1984) The auricular myocardiocytes of the heart constitute an endocrine organ. Characterization of a porcine cardiac peptide hormone, cardiodilatin-126. Cell Tissue Res 238:425–430
Guesdon J, Ternynck T, Avrameas S (1979) The use of avidinbiotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27:1131–1139
Horisberger M (1979) Evaluation of colloidal gold as a cytochemical marker for transmission and scanning electron microscopy. Biol Cell 36:253–258
Horisberger M (1981) Colloidal gold: a cytochemical marker for light and fluorescent microscopy and for transmisson and scanning electron microscopy. In: Johari Oin (ed) Scanning Electron Microscopy, 1981. SEM inc., AMF O'Hara, Chicago, Illinois, USA, pp 9–31
Hsu Su-Ming, Raine L, Fanger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580
Jacobowitz DM, Skofitsch G, Kciser HR, Eskay RL, Zamir N (1985) Evidence for the existence of atrial natriuretic factor-containing neurons in the rat brain. Neuroendocrinology 40:92–94
Jamieson JD, Palade GE (1964) Specific granules in atrial muscle cells. J Cell Biol 23:151–172
Kangawa K, Matsuo H (1984) Purification and complete amino acid sequence of α-human atrial natriuretic polypeptide (α- hANP). Biochem Biophys Res Commun 118:131–139
Kangawa K, Fukuda A, Kubota I, Hayashi Y, Matsuo H (1984) Identification in rat atrial tissue of multiple forms of natriuretic polypeptides of about 3,000 daltons. Biochem Biophys Res Commun 121:585–592
Kangawa K, Fukuda A, Matsuo H (1985) Structural identification of β- and γ-human atrial natriuretic polypeptides. Nature 313:31, 397–400
Kawata M, Ueda. S, Nakao K, Morii N, Kiso Y, Imura H, Sano Y (1985) Immunohistochemical demonstration of α-atrial natriuretic polypeptide-containing neurons in the rat brain. Histochemistry 83:1–3
Kisch B (1965) Further studies about the microbodies in the atria of the heart. Exp Med Surg 23:1–12
Metz J, Mutt V, Forssmann WG (1984) Immunohistochemical localization of cardiodilatin in myoendocrine cells of the cardiac atria. Anat Embryol 170:123–127
Miyata A, Kangawa K, Toshimori T, Hatoh T, Matsuo H (1985) Molecular forms of atrial natriuretic polypeptides in mammalian tissue and plasma. Biochem Biophys Res Commun 129:248–255
Miyata A, Kangawa K, Toshimori T, Masudo H, Hatho T, Matsuo H (1986) Molecular forms and distribution of atrial natriuretic polypeptides (ANPs) in rat tissues and plasma. Peptide Chemistry 1985: Y. Kiso (ed) Protein Research Foundation, Osaka pp 17–22
Palade GE (1961) Secretory granules in the atrial myocardium. Anat Rec 139:262
Saetersdal T, Rotevatn S, Myoklebust R, Degarden S (1980) Development of specific granules in embryonic cardiac myocytes of the human auricular wall. Anat Embryol 160:1–9
Saper CB, Standaert DG, Currie MG, Schwartz D, Geller DM, Needleman P (1985) Atriopeptin-immunoreactive neurons in the brain: Presence in cardiovascular regulatory areas. Science 227:1047–1049
Sissman NJ (1970) Developmental landmarks in cardiac morphogenesis: Comparative chronology. Am J Cardiol 25:141–148
Virágh Sz, Porte A (1973) On the impulse conducting system of the monkey heart (macaca mulatta). II. The atrio-venticular node and bundle. Z Zellforsch 145:363–388
Virágh Sz, Challice CE (1983) The development of the early atrioventricular conduction system in the embryonic heart. Can J Physiol Pharmacol 61:775–792
Author information
Authors and Affiliations
Additional information
This research was supported in part by Grants-in-Aid for Scientific research to C. Ōura from the Ministry of Education of Japan (Nos. 5957009, 59570010)
Rights and permissions
About this article
Cite this article
Toshimori, H., Toshimori, K., Ōura, C. et al. Immunohistochemical study of atrial natriuretic polypeptides in the embryonic, fetal and neonatal rat heart. Cell Tissue Res. 248, 627–633 (1987). https://doi.org/10.1007/BF00216493
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00216493