Summary
The development of epidermis and epidermal appendages from dissociated cells of neonate mouse skin was examined by transplantation of cell suspensions to subdermally prepared, protected graft beds. Using Ficol gradients and culture procedures, we prepared subfractions of primary cell suspensions consisting of essentially pure epidermal cells or fibroblasts.
Reformation of an epithelium structurally similar to the epidermis was observed from transplanted epidermal-cell suspensions, but formation of hair follicles and development of normal epidermal microarchitecture was observed only when epidermal cells were transplanted together with cells of dermal origin. This pattern was observed following transplantation of either fresh-cell isolates or cells cultured up to 7 days prior to transplantation.
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References
Banks-Schlegel S, Green H (1980) Formation of epidermis by serially cultivated human epidermal cells transplanted as an epithelium to athymic mice. Transplantation 29:308–313
Billingham RE, Reynolds J (1952) Transplantation studies on sheets of pure epidermal epithelium and on epidermal cell suspensions. Br J Plast Surg 5:2536
Briggaman RA, Wheeler CE (1968) Epidermal-dermal interactions in adult human skin: Role of dermis in epidermal maintenance. J Invest Dermatol 51:454
Briggaman RA, Wheeler CE (1971) Epidermal-dermal interactions in adult human skin. II. The nature of the dermal influence. J Invest Dermatol 56:18–26
Christophers E (1971) Cellular architecture of the stratum corneum. J Invest Dermatol 56:165–169
Doran TI, Vidrich AI, Sun TT (1980) Instrinsic and extrinsic regulation of the differentiation of skin, corneal and esophageal epithelial cells. Cell 22:1725
Fuchs E, Green H (1980) Changes in keratin gene expression during terminal differentiation of the keratinocyte. Cell 19:1033–1042
Fusenig NE, Worst PKM (1975) Mouse epidermal cell cultures. II. Isolation, characterization, and cultivation of epidermal cells from perinatal mouse skin. Exp Cell Res 93:443–457
Fusenig NE, Amer SM, Boukamp P, Worst PKM (1978) Characteristics of chemically transformed mouse epidermal cells in vitro and in vivo. Bull Canc 65:271–280
Fusenig NE, Breitkreutz D, Boukamp P, Lueder M, Irmscher G, Worst PKM (1979) Chemical carcinogenesis in mouse epidermal cell cultures. In: Franks LM, Wigley CB (eds) Neoplastic transformation in differentiated epithelial cell systems in vitro. Academic Press, London, pp 37–98
Fusenig NE, Valentine EA, Worst PKM (1980) Growth behavior of normal and transformed mouse epidermal cells after reimplantation in vivo. In: Richards RJ, Rajan KT (eds), Tissue culture in medical research (II). Pergamon Press, Oxford New York, pp 87–95
Fusenig NE, Breitkreutz D, Lueder M, Boukamp P, Hornung J, Worst PKM (1981) Keratinization and structural organization in epidermal cell cultures. In: Schweiger HG (ed) International cell biology 1981/1982. Springer Verlag, Heidelberg, pp 1004–1014
Green H (1977) Terminal differentiation of cultured human epidermal cells. Cell 11:405–416
Green H, Kehinde O, Thomas J (1979) Growth of cultured human epidermal cells into multiple epithelia suitable for grafting. Proc Natl Acad Sci USA 76:5665–5668
Karasek MA (1968) Growth and differentiation of transplanted epithelial cultures. J Invest Dermatol 51:247–252
Lillie JH, MacCallum DK, Jepsen A (1980) Fine structure of subcultivated stratified squamous epithelium grown on collagen rafts. Exp Cell Res 125:153–165
Mackenzie IC (1972) Epidermal wound healing. Maibach HI, Rovee DT (eds) Yearbook Medical Publishers Chicago, pp 5–25
Mackenzie IC, Linder JE (1973) An examination of cellular organization within the stratum corneum by a silver staining method. J Invest Dermatol 61:245–250
Mackenzie IC, Zimmerman K (1981) The development of ordered structure in neonate rat epidermis. JInvest Dermatol 77:278–284
Mackenzie IC, Dabelsteen E, Roed-Petersen B (1979) A method for studying epithelial-mesenchymal interactions in human oral mucosal lesions. Scand J Dent Res 87:234–243
Marcelo CL, Kim YG, Kaine JL, Voorhees JJ (1978) Stratification, specialization, and proliferation of primary keratinocyte cultures. J Cell Biol 79:356–370
Medawar PB (1948) The cultivation of adult mammalian skin epithelium in vitro. Quart J Microsc Sci 89:187–196
Menton DN (1976) A liquid film model of tetrakaidecahedral packing to account for the establishment of epidermal cell columns. J Invest Dermatol 66:283–291
Moscona A (1961) Rotation-mediated histogenetic aggregation of dissociated cells. Exp Cell Res 22:445–475
Rheinwald JG (1979) The role of terminal differentiation in the finite culture lifetime of the human epidermal keratinocyte. Int Rev Cytol 10:25–33
Rheinwald J, Green H (1975) Serial cultivation of strains of human epidermal keratinocytes: the formation of keratinizing colonies from single cells. Cell 6:331–334
Sengel P (1976) Morphogenesis of skin. Cambridge University Press, Cambridge London New York Melbourne
Van Scott EJ, Reinertson RP (1961) The modulating influence of stromal environment on epithelial cells studied in human autotransplants. J Invest Dermatol 36:109–131
Voigt WH, Fusenig NE (1979) Organotypic differentiation of mouse keratinocytes in cell culture: a light- and electronmicroscopic study. Biol Cellul 34:111–118
Worst PKM, Fusenig NE (1973a) A tissue antigen on the surface of cultivated mouse epidermal cells detected by mixed hemadsorption technique. J Invest Dermatol 61:277–281
Worst PMK, Fusenig NE (1973b) Histocompatibility antigens on the surface of cultivated epidermal cells from mouse embryo skin. Transplantation 15:375–383
Worst PKM, Valentine EA, Fusenig NE (1974) Formation of epidermis after reimplantation of pure primary epidermal cell cultures from perinatal mouse skin. J NatI Cancer Inst 53:1061–1064
Yuspa SH, Morgan DL, Walker RJ, Bates RR (1970) The growth of fetal mouse skin in cell culture and transplantation to f1 mice. J Invest Dermatol 55:379–389
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Part of the work was performed when one of us (I.C. Mackenzie) was guest scientist at the DKFZ.
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Worst, P.K.M., Mackenzie, I.C. & Fusenig, N.E. Reformation of organized epidermal structure by transplantation of suspensions and cultures of epidermal and dermal cells. Cell Tissue Res. 225, 65–77 (1982). https://doi.org/10.1007/BF00216219
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DOI: https://doi.org/10.1007/BF00216219