Abstract
The fine structure of blood cells, with particular reference to basophils, was studied in broiler chickens before and after they had been subjected to heat stress. Granule counts in basophils after heat treatment showed a significant reduction in their total numbers compared with pre-heat treatment. This depletion of granules corresponded with evidence of degranulation seen at the ultrastructural level. Of the three types of basophilic granules described, only the type I electron-dense, homogeneous variety of granule was significantly reduced in number.
After heat stress, there was a significant increase in heterophil lobulation. In several birds there was also evidence of cytoplasmic fragmentation in the form of portions of cells containing granules but no nuclei, lying free in the circulation. Damaged mitochondria, not seen before heat treatment, were frequently observed in the cells. Monocytes also showed significant increases in cytoplasmic lipid droplets after heat stress compared with pre-heat treatment, suggesting the onset of possibly early fatty degeneration. However, there was no evidence of increased pseudopodia or pinocytotic activity in these cells. With heat stress, there was a significant reduction in the numbers of electron-dense granules in the small lymphocytes. Despite there being a smaller proportion of circulating eosinophils after heat stress, which was considered to be the result of an immediate stress response, no significant differences were evident between pre- and post-heat treatment in the cells.
The red cells and thrombocytes from post-heat-stressed broilers were significantly longer and thinner compared with pre-heat treatment due possibly to dehydration. The dimensional and ultrastructural changes identified in the cells after heat stress question their effective functional ability in raising an immune response under such circumstances.
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References
Anderson DR (1965) A method of preparing peripheral leukocytes for electron microscopy. J Ultrastruct Res 13:263–268
Boyden S (1962) The chemotactic effect of mixtures of antibody and antigen on polymorphonuclear leukocytes. J Exp Med 115:253–466
Chand N, Eyres P (1978) Rapid method for basophil count in domestic fowl. Avian Dis 22:639–645
Dvorak AM (1988a) Morphologic and immunologic characterisation of human basophils, 1879 to 1985. J Immunol Immunopharmacol 8:50–83
Dvorak AM (1988b) In: Holgate ST (ed) Immunology and medicine: mast cells, mediators and disease. The fine structure of human basophils and mast cells. Kluwer Academic Dordrecht, pp29–97
Gross WB (1984) Differential and total avian blood cell counts by the haemocytometer method. Avian Exot Pract 1:31–36
Gross WB (1989) Factors affecting chicken thrombocyte morphology and the relationship with heterophil/lymphocyte ratios. Br Poult Sci 30:919–925
Gross WB, Siegel HS (1983) Evaluation of the heterophil-Lymphocyte ratio as a measure of stress in chickens. Avian Dis 27:972–979
Holroyde MC, Eyre P (1975) Immunologic release of histamine from bovine polymorphonuclear leukocytes. Am J Vet Res 36:1801–1802
Lucas AM, Jamroz C (1961) Atlas of avian hematology. United States Department of Agriculture, Washington, pp 91, 155
Lutas EM, Zucker-Franklin D (1977) Formation of lipid inclusions in normal human leukocytes. Blood 49:309–320
Maxwell MH (1978) Safer substitutes for xylene and propylene oxide in histology, haematology, and electron microscopy. Med Lab Sci 35:401–403
Maxwell MH (1981) Production of a Heinz body anaemia in the domestic fowl after ingestion of dimethyl disulphide: a haematological and ultrastructural study. Res Vet Sci 30:233–238
Maxwell MH, Burns RB (1982) Experimental eosinophilia in domestic fowls and ducks following horse serum stimulation. Vet Res Commun 5:369–376
Maxwell MH, Trejo F (1970) The ultrastructure of white blood cells and thrombocytes of the domestic fowl. Br Vet J 126:583–592
Maxwell MH, Dolan TT, Mbugua HCW (1989) An ultrastructural study of an ascitic syndrome in young broilers reared at high altitude. Avian Pathol 18:481–494
Maxwell MH, Hocking PM, Robertson GW (1992) Differential leucocyte responses to various levels of food restriction in broilers, turkeys and ducks. Br Poult Sci 33:177–187
Maxwell MH, Robertson GW, Spence S (1986) Studies on an ascitic syndrome in young broilers 1. Haematology and Pathology. Avian Pathol 15:511–524
Maxwell MH, Robertson GW, Spence S et al. (1990) Comparison of haematological values in restricted — and ad libitum — fed domestic fowls: white blood cells and thrombocytes. Br Poult Sci 31:399–405
Mitchell MA, Maxwell MH, Carlisle AJ et al. (1993) Relative basophilia: an index of severe thermal stress in the domestic fowl. Comp Haematol Int (in preparation)
Reynolds ES (1963) The use of lead citrate at high pH as an electronopaque stain in electron microscopy. J Cell Biol 17:208–212
Shane SM (1988) Hot climates and ventilation. Managing poultry in hot climates. Zootecnica April, 37–40
Watson ML (1958) Staining of tissue sections for electron microscopy with heavy metals. J Biophys Biochem Cytol 4:475–478
Zucker-Franklin D, Graves MF, Grossi CE et al. (1981) Atlas of blood cells, function and pathology: lymphocytes. Lea and Febiger, Philadelphia, pp 352–353
Zucker-Franklin D, Grusky G, Marcus A (1978) Transformation of monocytes into ‘fat’ cells. Lab Invest 38:620–628
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Maxwell, M.H., Robertson, G.W., Mitchell, M.A. et al. The fine structure of broiler chicken blood cells, with particular reference to basophils, after severe heat stress. Comparative Haematology International 2, 190–200 (1992). https://doi.org/10.1007/BF00216094
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DOI: https://doi.org/10.1007/BF00216094