Summary
Bovine placental lactogen activity is shown by immunogold electron microscopy to be restricted to (a) the granules and the Golgi body from which they form in the bovine fetal trophectodermal binucleate cell, and (b) granules of similar size and staining reaction in trinucleate “giant” cells found in the maternal uterine epithelium throughout pregnancy. These results support the hypothesis that a fetal binucleate cell forms a maternal giant cell by migration to and fusion with a uterine epithelial cell.
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References
Arima Y, Bremel RD (1983) Purification and characterisation of bovine placental lactogen. Endocrinology 113:2186–2184
Beckers JF, Fromont-Lienard CH; Van der Zwalmen P, Wouters-Ballman P, Ectors F (1980) Isolement d'une hormone placentaire bovine présentant un réactivité analogue à la prolactine et à l'hormone de croissance. Ann Vet Med 124:585–601
Beckers JF, Decoster R, Wouters-Ballman P, Fromont-Lienard CH, Van der Zwalmen P, Ectors F (1982) Dosage radioimmunologique de l'hormone placentaire somatotrope et mammotrope bovine. Ann Vet Med 126:9–21
Bjorkman N (1968) Fine structure of cryptal and trophectodermal giant cells in the bovine placentome. J Ultrastruct Res 24:249–258
Blank MS, Chan JSD, Friesen HG (1977) Placental lactogens, new developments. J Steroid Biochem 8:403–414
Chan JSD, Robertson HA, Friesen HG (1978) Maternal and fetal concentrations of ovine placental lactogen measured by radioimmunoassay. Endocrinology 102:1606–1613
Grandis AS, Handwerger S (1983) Differential effects of ornithine on placental lactogen and growth hormone secretion in the pregnant ewe and fetus. J Endocrinol 97:175–178
Hall J, Talamantes F (1984) Immunocytochemical localisation of mouse placental lactogen in the mouse placenta. J Histochem Cytochem 32:379–381
King GJ, Atknson BA; Robertson HA (1979) Development of the bovine placentome during the second month of gestation. J Reprod Fertil 55:173–180
Lee CS, Gogolin-Ewens K, White TR, Brandon MR (1985) Studies on the distribution of binucleate cells in the placenta of the sheep with a monoclonal antibody SBU-3. J Anat 140:565–576
Martal J, Djiane J, Dubois MP (1977) Immunofluorescent localisation of ovine placental lactogen. Cell Tissue Res 184:427–433
Murthy GS, Schellenberg C, Friesen HG (1982) Purification and characterisation of bovine placental lactogen. Endocrinology 111:2117–2124
Porter DG (1980) Felomaternal relationships: the actions and the control of certain hormones. Placenta 1:259–274
Sciarra JJ, Kaplan SL, Grumbach MM (1963) Localisation of antihuman growth hormone serum within the human placenta: evidence for a human chorionic ‘growth hormone prolactin’. Nature 199:1005–1006
Spaur RC, Moriarty GC (1977) Improvements of glycolmethacrylate: its use as on embedding medium for electron microscopic studies. J Histochem Cytochem 25:163–174
Talamantes F, Ogren L, Markoff E, Woodard S, Madrid J (1980) Phylogenetic distribution, regulation of secretion and prolactin like effects of placental lactogens. Fed Proc 39:2582–2587
Theodosis DT, Wooding FBP, Sheldrick EL, Flint APF (1986) Ultrastructural localisation of oxytocin and neurophysin in the ovine corpus luteum. Cell Tissue Res 243:129–135
Verstegen J, Fellman D, Beckers JF (1985) Immunodetection of bovine chorionic somatomammotropin. Acta Endocrinol 109:403–410
Wooding FBP (1980) Electron microscopic localisation of binucleate cells in the sheep placenta using phosphotungstic acid. Biol Reprod 22:357–365
Wooding FBP (1981) Localisation of ovine placental lactogen in sheep placentomes by electron microscope immunocytochemistry. J Reprod Fertil 62:15–19
Wooding FBP (1982) The role of the binucleate cell in ruminant placental structure. J Reprod Fertil [Suppl] 31:31–39
Wooding FBP (1984) Role of binucleate cells in fetomaternal cell fusion at implantation in the sheep. Am J Anat 170:233–250
Wooding FBP, Wathes DC (1980) Binucleate cell migration in the bovine placentome. J Reprod Fertil 59:425–430
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Wooding, F.B.P., Beckers, J.F. Trinucleate cells and the ultrastructural localisation of bovine placental lactogen. Cell Tissue Res. 247, 667–673 (1987). https://doi.org/10.1007/BF00215761
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DOI: https://doi.org/10.1007/BF00215761