Summary
Nervous elements in the periodontal ligament and dental pulp of rat incisors were investigated by means of immunohistochemistry for neurofilament protein (NFP) and glia-specific S-100 protein. The periodontal ligament in the incisors was densely innervated by NFP-immunoreactive nerve fibers; the distribution of the nerve fibers and their terminations differed markedly from those in molars. NFP-positive, thick nerve bundles entered the lingual periodontal ligament through slits located in the mid-region of the alveolar socket, and immediately formed numerous Ruffini-like corpuscles. In the labial periodontal ligament, all of the NFP-immunoreactive nerve fibers terminated in free endings. The restricted location of the stretch receptor, Ruffini-like corpuscle, in the lingual periodontal ligament appears to be an essential element, because this region is regularly extended during mastication. The nervous elements were restricted to the alveolar half of the periodontal ligament in every region; they avoided the dental half of the periodontal ligament, which presumably moves continuously with the tooth. Pulpal nerve fibers in incisors also showed a characteristic distribution different from those in molars; individual nerve fibers with beaded structures ran in the center of the pulp toward the incisai edge, and did not form the subodontoblastic nerve plexus of Raschkow.
Immunostaining for S-100 protein revealed a distribution pattern of nervous elements similar to that for NFP, suggesting that the nerves supplying the periodontal ligament and dental pulp were mostly covered by a Schwann sheath.
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References
Beertsen W (1975) Migration of fibroblasts in the periodontal ligament of the mouse incisor as revealed by autoradiography. Arch Oral Biol 20:659–666
Beertsen W, Everts V, Hooff A (1974) Fine structure and possible function of cells containing leptomeric organelles in periodontal ligament of the rat incisor. Arch Oral Biol 19:1099–1100
Berkovitz BKB, Shore RC, Moxham BJ (1983) The occurrence of a lamellated nerve terminal in the periodontal ligament of the rat incisor. Arch Oral Biol 28:99–101
Bernick S (1956) The innervation of the teeth and periodontium of the rat. Anat Rec 125:185–205
Bock E (1978) Nervous system specific proteins. J Neurochem 30:7–14
Byers MR (1984) Dental sensory receptors. Int Rev Neurobiol 25:39–94
Byers MR (1985) Sensory innervation of periodontal ligament of rat molars consists of encapsulated Ruffini-like mechanoreceptors and free nerve endings. J Comp Neurol 231:500–518
Byers MR, Chan KY (1984) Comparison of nociceptors and proprioceptors in pulp and periodontal ligament of rat incisors using radioautography. HRP-cytochemistry and electron microscopy. Pain Abstra. (IV World Congress on Pain). Pain (Suppl 2):59
Byers MR, Holland GR (1977) Trigeminal nerve endings in gingiva, junctional epithelium and periodontal ligament of rat molars as demonstrated by autoradiography. Anat Rec 188:509–523
Byers MR, Kish SJ (1976) Delineation of somatic nerve endings in rat teeth by radioautography of axon-transported protein. J Dent Res 55:419–425
Chambers MR, Andres KH, During M, Iggo A (1972) Structure and function of the slowly adapting type II mechanoreceptor in hairy skin. Q J Exp Physiol 57:417–445
Cocchia D, Michetti F (1981) S-100 antigen in satellite cells of the adrenal medulla and the superior cervical ganglion of the rat. An immunochemical and immunocytochemical study. Cell Tissue Res 215:103–112
Dalsgaard C-J, Björklund H, Jonsson C-E, Hermansson A, Dahl D (1984) Distribution of neurofilament-immunoreactive nerve fibers in human skin. Histochemistry 81:111–114
Eccles JD (1965) The development of the periodontal membrane in the rat incisor. Arch Oral Biol 19:127–133
Everts V, Beertsen W, Hooff A (1977) Fine structure of an end organ in the periodontal ligament of the mouse incisor. Anat Rec 189:73–90
Funakoshi M, Amano N (1974) Periodontal jaw muscle reflexes in the albino rat. J Dent Res 53:598–605
Hattyasy D (1956) Innervation of the continuously growing incisor. Nature 178:416
Hattyasy D (1959) Zur Frage der Innervation der Zahnwurzelhaut. Z Mikrosk Anat Forsch 65:413–433
Iwanaga T, Fujita T, Takahashi Y, Nakajima T (1982) Meissner's and Pacinian corpuscles as studied by immunohistochemistry for S-100 protein, neuron specific enolase and neurofilament protein. Neurosci Lett 31:117–121
Kizior JE, Cuzzo JW, Bowmann DC (1968) Functional and histologic assessment of the sensory innervation of the periodontal ligament of the cat. J Dent Res 47:59–64
Maeda T, Iwanaga T, Fujita T, Kobayashi S (1985) Immunohistochemical demonstration of the nerves in human dental pulp with antisera against neurofilament protein and glia-specific S-100 protein. Arch Histol Jpn 48:123–129
Maeda T, Iwanaga T, Fujita T, Kobayashi S (1986) Immunohistochemical demonstration of nerves in the predentin and dentin of human third molars with the use of an antiserum against neurofilament protein (NFP). Cell Tissue Res 243:469–475
Maeda T, Iwanaga T, Takahashi Y, Fujita T, Kobayashi S (1987) Distribution of nerve fibers immunoreactive to neurofilament protein in rat molars and periodontium. Cell Tissue Res 249:13–23
Masuda T, Sakimura K, Yoshida Y, Kuwano R, Isobe T, Okuyama T, Takahashi Y (1983) Developmental changes in the translatable mRNA for β subunit of S-100 protein in rat brain. Biochem Biophys Acta 740:249–254
Munger BL, Halata Z (1983) The sensory innervation of primate facial skin. I. Hairy skin. Brain Res Rev 5:45–80
Perdrauv JR (1921) The silver reduction method for the demonstration of connective tissue fibers. J Pathol 24:117
Photo P, Antila R (1968) Demonstration of adrenergic nerve fibers in human dental pulp by histochemical fluorescence method. Acta Odontol Scand 26:137–144
Schour I, Massler M (1962) The teeth, In: Farris EJ, Griffith JQ (eds) The rat in laboratory investigation. Hanfner Publishing Company, New York, pp 104–165
Seiger A, Dahl D, Ayer-Le Lievre C, Björklund H (1984) Appearance and distribution of neurofilament protein immunoreactivity in iris nerves. J Comp Neurol 223:457–470
Shore RC, Berkovitz BKB (1978) Model to explain differential movement of periodontal fibroblasts. Arch Oral Biol 23:507–509
Sloan P, Shellis RP, Berkovitz BKB (1976) Effect of specimen preparation on the appearance of the rat periodontal ligament in the scanning electron microscope. Arch Oral Biol 21:633–635
Steenberghe D (1979) The structure and function of periodontal innervation. A review of the literature. J Periodont Res 14:185–203
Stefansson K, Wollmann RL, Moore BW (1982) Distribution of S-100 protein outside the central nervous system. Brain Res 234:309–317
Sternberger LA (1974) Immunohistochemistry, 1st ed. Prentice Hall Inc, Englewood Cliffs, New Jersey
Tsuchiya H (1983) Studies on the periodontal fiber architecture of the continuously erupting tooth. Jpn J Oral Biol 25:1073–1089
Yen SH, Fielder KL (1981) Antibodies to neurofilament, glia filament and fibroblast intermediate filament proteins bind to different cell types of the nervous system. J Cell Biol 88:115–126
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Sato, O., Maeda, T., Kobayashi, S. et al. Innervation of periodontal ligament and dental pulp in the rat incisor: An immunohistochemical investigation of neurofilament protein and glia-specific S-100 protein. Cell Tissue Res. 251, 13–21 (1988). https://doi.org/10.1007/BF00215442
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DOI: https://doi.org/10.1007/BF00215442