Summary
Barley middle-repeat sequences were screened for their ability to discriminate 51 barley commercial varieties. Two hordein clones, a clone encoding a leaf-specific thionin, a desiccation induced cDNA clone, a clone coding for 5S-rRNA and one corresponding to ubiquitin genes were tested. A very sensitive RFLP technique including four cutter restriction enzymes and denaturing 4% polyacrylamide gels were used to evidence the highest level of polymorphism.
The RFLP data were analyzed by computer. Some probe/enzyme combinations were able to differentiate a large number of the cultivars tested, whereas three probe/enzyme combinations succeeded in identifying all the varieties. The use of this RFLP method can thus be suggested for cultivar identification in barley.
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References
Beckmann JS, Soller M (1983) Restriction fragment length polymorphisms in genetic improvement: methodologies, mapping and costs. Theor Appl Genet 67:35–43
Bohlmann H, Apel K (1987) Isolation and characterization of cDNAs coding for leaf-specific thionins closely related to the endosperm-specific hordothionin of barley (Hordeum vulgare L.). Mol Gen Genet 207:446–454
Bunce NAC, Forde BG, Kreis M, Shewry PR (1986) DNA restriction fragment length polymorphism at hordein loci: application to identifying and fingerprinting barley cultivars. Seed Sci Technol 14:419–429
Cooke RJ, Morgan AG (1986) A revised classification of barley cultivars using a standard reference electrophoresis method. J Nat Inst Agric Bot 17:169–178
Feinberg AP, Vogelstein B (1984) A technique for radiolabelling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 137:266–267
Forde BG, Kreis M, Williamson MS, Fry RP, Pywell J, Shewry PR, Bunce NAC, Miflin BJ (1985) Short tandem repeats shared by B- and C-hordein cDNAs suggest a common evolutionary origin for two groups of cereal storage protein genes. EMBO J 4:9–15
Gausing K, Barkardottir R (1986) Structure and expression of ubiquitin genes in higher plants. Eur J Biochem 158:57–62
Gebhardt C, Salamini F (1992) RFLP analysis of plant genomes and its application to plant breeding. Int Rev Cytol 135:201–237
Gebhardt C, Blomendahl C, Schachtschabel U, Debener T, Salamini F, Ritter E (1989a) Identification of 2n breeding lines and 4n varieties of potato (Solarium tuberosum, ssp. tuberosum) with RFLP fingerprints. Theor Appl Genet 78:16–22
Gebhardt C, Ritter E, Debener T, Schachtschabel U, Walkemeier B, Uhrig H, Salamini F (1989b) RFLP analysis and linkage mapping in Solamum tuberosum. Theor Appl Genet 78:65–75
Görg R, Schachtschabel U, Ritter E, Salamini F, Gebhardt C (1992) Discrimination among 136 tetraploid potato varieties by fingerprints using highly polymorphic DNA markers. Crop Sci 32:815–819
Johns MA, Strommer JN, Freeling M (1983) Exceptionally high levels of restriction site polymorphism in DNA near the maize Adh1 gene. Genetics 105:733–743
Kolchinsky A, Kanazin V, Yakovleva E, Gazumyan A, Kole C, Ananiev E (1990) 5S-RNA genes of barley are located on the second chromosome. Theor Appl Genet 80:333–336
Kreis M, Shewry PR (1992) The control of protein synthesis in developing barley seeds. In: Shewry PR (ed) Barley: genetics, biochemistry, molecular biology and biotechnology. CAB International, England pp 319–333
Molina-Cano JL, Elena Rosselló JM (1978) A further contribution to the classification of barley cultivars: use of numerical taxonomy and biochemical methods. Seed Sci Technol 6:593–615
Nielsen G, Johansen HB (1986) Proposal for the identification of barley varieties based on the genotypes for 2 hordein and 39 isoenzyme loci of 47 reference varieties. Euphytica 35:717–728
Sanguineti MC, Conti S, Landi P (1979) ‘Opale’ (IABO 82) ‘Onice’ (IABO 33): due nuove varietá di orzo. Sementi Elette 2:25–33
Shewry PR, Faulks AJ, Pickering RA, Jones JT, Finch RA, Miflin BJ (1980) The genetic analysis of barley storage proteins. Heredity 44:383–389
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Pecchioni, N., Stanca, A.M., Terzi, V. et al. RFLP analysis of highly polymorphic loci in barley. Theoret. Appl. Genetics 85, 926–930 (1993). https://doi.org/10.1007/BF00215030
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DOI: https://doi.org/10.1007/BF00215030