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The 180-kD component of the neural cell adhesion molecule N-CAM is involved in cell-cell contacts and cytoskeleton-membrane interactions

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Summary

N-CAM180, the molecular form of the three neural cell adhesion molecules (N-CAM) with the largest cytoplasmic domain, is accumulated at sites of cell-cell contact (cell bodies, neurites, growth cones) in cultures of neuroblastoma and cerebellum. At these sites the cytoskeletonmembrane linker protein brain spectrin and actin are also accumulated. Brain spectrin copurifies with N-CAM180 by immunoaffinity chromatography and binds specifically to N-CAM180 but not to N-CAM140 or N-CAM120 in a solid-phase binding test. These observations indicate an association of N-CAM180 with the cytoskeleton in vivo. This association may underlie the reduced lateral mobility of N-CAM180 in the surface membrane compared to N-CAM140 (Pollerberg et al. 1986). Together with the fact that N-CAM180 is only expressed after termination of neuron migration in vivo (Persohn and Schachner, unpublished) these results suggest a role for N-CAM180 in stabilization of cell contacts.

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Authors and Affiliations

  1. Department of Neurobiology, University of Heidelberg, Heidelberg, Federal Republic of Germany

    G. Elisabeth Pollerberg &  Melitta Schachner

  2. Department of Anatomy, The School of Medicine, University of North Carolina, Chapel Hill, North Carolina, USA

    Keith Burridge

  3. Department of Physiology, The Milton Hershey Medical Center, Hershey, Pennsylvania, USA

    Keith E. Krebs & Steven R. Goodman

Authors
  1. G. Elisabeth Pollerberg
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  2. Keith Burridge
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  3. Keith E. Krebs
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  4. Steven R. Goodman
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  5. Melitta Schachner
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Pollerberg, G.E., Burridge, K., Krebs, K.E. et al. The 180-kD component of the neural cell adhesion molecule N-CAM is involved in cell-cell contacts and cytoskeleton-membrane interactions. Cell Tissue Res. 250, 227–236 (1987). https://doi.org/10.1007/BF00214676

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  • DOI: https://doi.org/10.1007/BF00214676

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