Summary
Pre-embedding immunocytochemistry was performed on vibratome sections of the hypothalamus of lactating rats using antiserum directed against oxytocin. Electron microscopy revealed that numerous immunopositive somata and dendrites in the supraoptic nucleus were in direct apposition, without glial interposition; a number of them were also bridged by “double” synapses. The observations support the contention that the ultrastructural reorganisation of the nucleus apparent at lactation affects the magnocellular neurones secreting oxytocin.
References
Hatton GI, Tweedle CD (1982) Magnocellular neuropeptidergic neurones in the hypothalamus: increases in membrane apposition and number of specialized synapses from pregnancy to lactation. Brain Res Bull 8:197–204
Piekut DT, Casey SM (1983) Penetration of immunoreagents in vibratome-sectioned brain: a light and electron microscope study. J Histochem Cytochem 31:669–674
Poulain DA, Wakerley J (1982) Electrophysiology of hypothalamic magnocellular neurones secreting oxytocin and vasopressin. Neuroscience 7:773–808
Rhodes CH, Morrell JI, Pfaff DW (1981) Immunohistochemical analysis of magnocellular elements in rat hypothalamus: distribution and number of cells containing neurophysin, oxytocin and vasopressin. J Comp Neurol 198:45–64
Sternberger LA (1974) Immunocytochemistry. Prentice Hall Inc, Englewood Cliffs, NJ
Swaab DF, Nijveld F, Pool CW (1975) Distribution of oxytocin and vasopressin in the rat supraoptic and paraventricular nucleus. J Endocrinol 67:461–462
Theodosis DT (1982) Secretion-related accumulation of horseradish peroxidase in magnocellular cell bodies of the rat supraoptic nucleus. Brain Res 233:3–16
Theodosis DT, Poulain DA (1983) Evidence for structural plasticity in the supraoptic nucleus of the rat hypothalamus in relation to gestation and lactation. Neuroscience: In press
Theodosis DT, Poulain DA, Vincent JD (1981) Possible morphological bases for synchronisation of neuronal firing in the rat supraoptic nucleus during lactation. Neuroscience 6:919–929
Vandesande F, Dierickx K (1975) Identification of the vasopressin-producing and of the oxytocin-producing neurones in the hypothalamic magnocellular neural system of the rat. Cell Tissue Res 164:153–162
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Theodosis, D.T., Poulain, D.A. Evidence that oxytocin-secreting neurones are involved in the ultrastructural reorganisation of the rat supraoptic nucleus apparent at lactation. Cell Tissue Res. 235, 217–219 (1984). https://doi.org/10.1007/BF00213745
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DOI: https://doi.org/10.1007/BF00213745