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In vitro spermatogenesis in Drosophila

I. Development of isolated spermatocyte cysts from wild-type D. hydei

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Summary

In vitro spermatogenesis of isolated single spermatocyte cysts of Drosophila hydei was studied by microscopic observations and time-lapse cinematography. Cysts of spermatocytes isolated during diplotene develop as far as the coiling stage of spermatid differentiation. The existence of an interphase between meiosis I and meiosis II is, for the first time, documented. Meiosis, Nebenkern formation, and elongation of spermatids occur just as in D. melanogaster; however, an individualization cone, as described for D. melanogaster, can not be detected.

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  1. Institut für Genetik, Universität Düsseldorf, Düsseldorf, Bundesrepublik Deutschland

    Winfrid Liebrich

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  1. Winfrid Liebrich
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Liebrich, W. In vitro spermatogenesis in Drosophila . Cell Tissue Res. 220, 251–262 (1981). https://doi.org/10.1007/BF00210507

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