Summary
The uptake of γ-aminobutyric acid (GABA) in the thyroid gland of the rat was studied autoradiographically following in vitro incubation.
High-affinity GABA uptake was localized in follicle cells, whereas C cells (parafollicular cells) in general did not accumulate GABA by high-affinity transport. The follicle cells were also the main sites of low-affinity GABA uptake. Additionally, some nerve fibres were found to accumulate GABA.
The predominant localization of GABA uptake in follicle cells is discussed in view of a presumed role of GABA in thyroid function.
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References
Amlacher E (1974) Autoradiographie in Histologie und Cytologie. Gustav Fischer, Stuttgart, p 62
Beart PM, Kelly JS, Schon F (1974) γ-Aminobutyric acid in the rat peripheral nervous system, pineal and posterior pituitary. Biochem Soc Trans 2:266–268
Bowery NG, Brown DA (1972) γ-Aminobutyric acid uptake by sympathetic ganglia. Nature 238:89–91
Christensen HN (1964) Relations in the transport of β-alanine and the α-amino acids in the Ehrlich cell. J Biol Chem 239:3584–3589
Chubb J, Huxtable R (1978) Isoproterenol-stimulated taurine influx in the perfused rat heart. Eur J Pharmacol 48:369–376
Crailsheim K, Gebauer H (1980) Glutamate decarboxylase in the rat thyroid gland. In: Brzin M, Sket D, Bachelard H (eds) Synaptic constituents in health and disease. Pergamon Press, Oxford, p 176
Curtis DR (1979) Gabergic transmission in the mammalian central nervous system. In: Krogsgaard-Larsen P, Scheel-Krüger J, Kofod H (eds) GABA-Neurotransmitters. Munksgaard, Copenhagen, pp 17–27
Friedman Y, Lang H, Burke G (1977) Inhibition of thyroid adenylate cyclase by thyroid hormone: A possible locus for the “short loop” negative feedback phenomenon. Endocrinology 101:858–868
Gaut ZN, Nauss CB (1976) Uptake of taurine by human blood platelets: A possible model for brain. In: Huxtable R, Barbeau A (eds) Taurine. Raven Press, New York, pp 91–98
Gebauer H (1977) Dual transport systems for γ-aminobutyric acid in the rat thyroid. Proc Austr Biochem Soc: p 14
Gebauer H (1979) Multiple transport systems for neurotransmitter amino acids in the rat thyroid. Acta Endocrinol (Kbh) 225:1
Gebauer H (1981) GABA transport in the rat thyroid. Naunyn-Schmiedeberg's Arch Pharmacol, in press
Gebauer H, Hagmüller K (1977) The uptake of 14C-γ-aminobutyric acid into various organs of the rat. Zool Jb Physiol 81:335–342
Gebauer H, Haas K (1980) γ-Aminobutyric acid transport in thyroids of triiodothyronine- and propylthiouracil-treated rats. Hoppe-Seyler's Z Physiol Chem 361:1248
Grosso DS, Roeske WR, Bressler R (1978) Characterization of a carrier-mediated transport system for taurine in the fetal mouse heart in vitro. J Clin Invest 61:944–952
Haber B, Kuriyama K, Roberts E (1970) An anion stimulated L-glutamic acid decarboxylase in non-neural tissues: occurrence and subcellular localization in mouse kidney and developing chick embryo brain. Biochem Pharmacol 19:1119–1136
Hammerman M, Sacktor B (1978) Transport of β-alanine in renal brush border membrane vesicles. Biochim Biophys Acta 509:338–347
Iversen LL, Kelly JS (1975) Uptake and metabolism of γ-amino-butyric acid by neurones and glial cells. Biochem Pharmacol 24:933–938
Lähdesmäki P, Oja SS (1973) On the mechanism of taurine transport at brain cell membranes. J Neurochem 20:1411–1417
Martin DL (1976) Carrier-mediated transport and removal of GABA from synaptic regions. In: Roberts E, Chase TN, Tower DB (eds) GABA in Nervous System Function. Raven Press, New York, pp 347–386
Martin DL, Shain W (1979) High-affinity transport of taurine and β-alanine and low-affinity transport of γ-aminobutyric acid by a single transport system in cultured glioma cells. J Biol Chem 254:7076–7084
Melander A, Nilsson E, Sundler F (1972) Sympathetic activation of thyroid hormone secretion in mice. Endocrinology 90:194–199
Melander A, Ericson LE, Sundler F, Ingbar SH (1974) Sympathetic innervation of the mouse thyroid and its significance in thyroid hormone secretion. Endocrinology 94:959–966
Melander A, Ericson LE, Sundler F, Westgren U (1975 a) Intrathyroidal amines in the regulation of thyroid activity. Rev Physiol Biochem Pharmacol 73:39–71
Melander A, Sundler F, Westgren U (1975b) Sympathetic innervation of the thyroid. Variation with species and with age. Endocrinology 96:102–106
Oja SS, Kontro P, Lähdesmäki P (1977) Amino acids as inhibitory neurotransmitters. Progr Pharmacol 1:1–119
Patel AJ, Balazs R, Richter D (1970) Contribution of the GABA bypath to glucose oxidation and the development of compartmentation in the brain. Nature 226:1160–1161
Pearse AGE (1976) Neurotransmission and the APUD concept. In: Coupland RE, Fujita T (eds) Chromaffin, enterochromaffin and related cells. Elsevier, Amsterdam, pp 147–154
Ritzen M, Hammarström L, Ullberg S (1964) Autoradiographic distribution of 5-hydroxytryptamine and 5-hydroxytryptophan in the mouse. Biochem Pharmacol 14:313–321
Rozen R, Tenenhouse HS, Scriver CR (1979) Taurine transport in renal brush-border membrane vesicles. Biochem J 180:245–248
Sawicki B (1971) Adaption of Solcia and Sampietro's method for stable metachromatic staining of “C” cells in histologie preparations of the thyroid. Folia morph (Warszawa) 30:404–409
Schousboe A (1979) Significance of the glial transport system for the inactivation of GABA and effect of structural analogues on the uptake. In: Krogsgaard-Larsen P, Scheel-Kruger J, Kofod H (eds) GABA-Neurotransmitter. Munksgaard, Copenhagen, pp 263–280
Schousboe A, Hertz L, Svenneby G (1977) Uptake and metabolism of GABA in astrocytes cultured from dissociated mouse brain hemispheres. Neurochem Res 2:217–229
Storm-Mathisen J, Fonnum F, Malthe-Sørensen D (1976) GABA uptake in nerve terminals. In: Roberts E, Chase TN, Tower DB (eds) GABA in nervous system function. Raven Press, New York, pp 387–394
Taniguchi H, Okada Y, Shimada C, Baba S (1977) GABA in pancreatic islets. Arch Histol Jpn. 40:87–97
Van Noorden S, Polak JM, Pearse AGE (1977) Single cellular origin of somatostatin and calcitonin in the rat thyroid gland. Histochemistry 53:243–247
Young JAC, Brown DA, Kelly JS, Schon F (1973) Autoradiographic localization of sites of (3H) γ-aminobutyric acid accumulation in peripheral autonomie ganglia. Brain Res 63:478–486
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Gebauer, H., Pabst, M.A. Autoradiographic localization of 3H-GABA uptake in the thyroid gland of the rat. Cell Tissue Res. 220, 873–879 (1981). https://doi.org/10.1007/BF00210468
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DOI: https://doi.org/10.1007/BF00210468