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Germinal centres and the B-cell system. V

Presence of germinal centre-precursor cells among lymphocytes of the thoracic duct in the rat

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Summary

Thoracic duct lymphocytes (TDL) were studied with respect to their capacity to give rise to germinal centres (GC) and to form primary antibody in an adoptive transfer system of the rat. Challenge with sheep erythrocytes (SRBC) 24h after lethal irradiation (900 rads) and syngeneic TDL reconstitution (108) lead to conspicuous GC activity already 7 days after transfer. In contrast, using syngeneic bone marrow (BM) in the adoptive transfer system, no GC formation was observed over the period studied (14 days after reconstitution). Reconstitution experiments using in vivo-separated T-TDL (1–5 % s-Ig+) and B-TDL (>90% s-Ig+) subpopulations, either separately or in combination, indicated that GC originate from B-TDL but require T-TDL for induction.

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Abbreviations

BM:

Bone Marrow

TDL:

Thoracic Duct Lymphocytes

GC:

Germinal Centre

SRBC:

Sheep Red Blood Cells

GCDC:

Germinal Centre-Derived Cells

GCPC:

Germinal Centre-Precursor Cells

DAB:

Mineral Salt Solution Dulbecco A + B

FCS:

Fetal Calf Serum

PALS:

Peri-Arteriolar Lymphocyte Sheath

AFCP:

Antibody-Forming Cell Precursors

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Authors and Affiliations

  1. Department of Histology, University of Groningen, Groningen, The Netherlands

    Nicolette A. Gastkemper, Auk S. Wubbena, Felix J. H. Gimbrère, Alexander de Graaff & Paul Nieuwenhuis

Authors
  1. Nicolette A. Gastkemper
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  2. Auk S. Wubbena
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  3. Felix J. H. Gimbrère
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  4. Alexander de Graaff
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  5. Paul Nieuwenhuis
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Gastkemper, N.A., Wubbena, A.S., Gimbrère, F.J.H. et al. Germinal centres and the B-cell system. V. Cell Tissue Res. 219, 281–289 (1981). https://doi.org/10.1007/BF00210148

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  • DOI: https://doi.org/10.1007/BF00210148

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