Abstract
Certain single cells in carrot (Daucus carota L.) suspension cultures react with the monoclonal antibody JIM8, and it has been proposed that these cells represent a transitional stage in somatic embryo formation. Shortly after isolation of the single cells by sieving, up to 80% of the cells react with JIM8. Within 4 d, JIM8 labelling becomes restricted to 1% of the single cells. To obtain evidence for the proposed correlation between expression of the JIM8 cell wall epitope and somatic embryo formation the developmental fate of carrot single cells labelled with JIM8 was determined by cell tracking. The results, obtained by recording 43 000 cells, show that only few JIM8-labelled cells give rise to embryos, and most somatic embryos develop from cells devoid of the JIM8 cell wall epitope. We therefore conclude that the presence of the JIM8 cell wall epitope does not coincide with the ability of single suspension cells to form embryos.
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Abbreviations
- 2,4-D:
-
2,4-dichlorophenoxyacetic acid
- AGP:
-
arabino galactan protein
- B5-0:
-
Gamborg's B5 medium
- B5-0.2:
-
Gamborg's B5 medium supplemented with 0.2 μM 2,4-D
- FITC:
-
fluoresceïn isothiocyanate
- PBS:
-
phosphate-buffered saline
References
Backs-Hüsemann D, Reinert J (1970) Embryobildung durch isolierte Einzelzellen aus Gewebekulturen von Daucus carota. Protoplasma 70: 49–60
De Jong AJ, Schmidt EDL, De Vries SC (1993) Early events in higherplant embryogenesis. Plant Mol Biol 22: 367–377
De Vries SC, Booij H, Meyerink P, Huisman G, Wilde HD, Thomas TL, Van Kammen A (1988) Acquisition of embryogenic potential in carrot cell-suspension cultures. Planta 176: 196–204
Dudits D, Györgyey J, Borge L, Bakó L (1995) Molecular biology of somatic embryogenesis. In: Thorpe TA (ed.) In vitro embryogenesis in plants. Kluwer Academic Publishers, Dordrecht, The Netherlands, pp 267–308
Egertsdotter U, Von Arnold S (1995) Importance of arabinogalactan proteins for the development of somatic embryos of Norway spruce (Picea abies). Physiol Plant 93: 334–345
Giorgetti L, Vergara MR, Evangelista M, LoSchiavo F, Terzi M, Nuti Ronchi V (1995) On the occurrence of somatic meiosis in embryogenic carrot cell cultures. Mol Gen Genet 246: 657–662
Guzzo F, Baldan B, Mariani P, Loschiavo F, Terzi M (1994) Studies on the origin of totipotent cells in explants of Daucus carota L. J Exp Bot 45: 1427–1432
Guzzo F, Baldan B, Levi M, Sparvoli E, LoSchiavo F, Terzi M, Mariani P (1995) Early cellular events during induction of carrot explants with 2,4-D. Protoplasma 185: 28–36
Knox JP, Linstead PJ, Peart J, Cooper C, Roberts K (1991) Developmentally regulated epitopes of the cell surface arabinogalactan proteins and their relation to root tissue pattern formation. Plant J 1: 317–326
Kreuger M, Van Holst G-J (1993) Arabinogalactan proteins are essential in somatic embryogenesis of Daucus carota L. Planta 189: 243–248
Kreuger M, Van Holst G-J (1995) Arabinogalactan-protein epitopes in somatic embryogenesis of Daucus carota L. Planta 197: 135–141
Nomura K, Komamine A (1985) Identification and isolation of single cells that produce somatic embryos at a high frequency in a carrot suspension culture. Plant Physiol 79: 988–991
Nuti Ronchi V, Giorgetti L, Tonelli M, Martini G (1992) Ploidy reduction and genome segregation in cultured carrot cell lines. II. Somatic meiosis. Plant Cell Tissue Organ Cult 30: 115–119
Pennell RI, Janniche L, Kjellbom P, Scofield GN, Peart JM, Roberts K (1991) Developmental regulation of a plasma membrane arabinogalactan protein epitope in oilseed rape flowers. Plant Cell 3: 1317–1326
Pennell RI, Janniche E, Scofield GN, Booij H, De Vries SC, Roberts K (1992) Identification of a transitional cell state in the developmental pathway to carrot somatic embryogenesis. J Cell Biol 119: 1371–1380
Serpe MD, Nothnagel EA (1994) Effects of Yariv phenylglycosides on Rosa cell suspensions: Evidence for the involvement of arabinogalactan-proteins in cell proliferation. Planta 193: 542–550
Spangenberg G, Koop H-U, Schweiger H-G (1985) Different types of protoplasts from Brassica napus L.: analysis of conditioning effects at the single cell level. Eur J Cell Biol 39: 41–45
Toonen MAJ, Hendriks T, Schmidt EDL, Verhoeven HA, Van Kammen, De Vries SC (1994) Description of somatic-embryo-forming single cells in carrot suspension cultures employing video cell tracking. Planta 194: 565–572
Widholm JM (1972) The use of fluorescein diacetate and phenosafranine for determining viability of cultured plant cells. Stain Technol 47: 189–194
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Toonen, M.A.J., Schmidt, E.D.L., Hendriks, T. et al. Expression of the JIM8 cell wall epitope in carrot somatic embryogenesis. Planta 200, 167–173 (1996). https://doi.org/10.1007/BF00208305
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DOI: https://doi.org/10.1007/BF00208305