Summary
Human cathepsin B gene (CTSB) has been mapped to two locations: 8p22 and 13q14. Here we confirm the chromosome 8 assignment by three independent methods: (1) analysis of human-hamster somatic cell hybrid DNA by polymerase chain reaction; (2) comparison of hybridization signals to cathepsin B in interphase nuclei of normal fibroblasts and fibroblasts with a chromosome 8 deletion; and (3) fluorescence in situ hybridization to metaphase spreads using cathepsin B cosmid clones. Our results indicate that human CTSB is located at 8p22-p23.1.
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Augereau P, Garcia M, Mattei MG, Cavailles V, DePadova F, Derocq D, Capony F, Ferrara P, Rochefort H (1988) Cloning and sequencing of the 52k cathepsin D complementary deoxyribonucleic acid of MCF-7 breast cancer cells and mapping on chromosome 11. Mol Endocrinol 2:186–192
Bachmann B, Luke W, Hunsmann G (1990) Improvement of PCR amplified DNA sequencing with the aid of detergents. Nucleic Acids Res 18:1309
Baldini A, Ward DC (1991) In situ hybridization banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies. Genomics 9:770–774
Bouyon C, Vignon X, Ferrara M, De Larocque A, Geneix A, Malet P (1989) In situ hybridization of a cathepsin B murine probe on the human chromosomes. Cytogenet cell Genet 51:966
Chan MM, Fong D (1988) Expression of human cathepsin B protein in Escherichia coli. FEBS Lett 239:219–222
Chan SJ, San Segundo B, McCormick MB, Steiner DF (1986) Nucleotide and predicted amino acid sequences of cloned human and mouse preprocathepsin B cDNAs. Proc Natl Acad Sci USA 83:7721–7725
Couvreur JM, Azuma T, Miller DA, Rocci M, Mohandas TK, Boudi FA, Taggart RT (1990) Assignment of cathepsin E (CTSE) to human chromosome region 1q31 by in situ hybridization and analysis of somatic cell hybrids. Cytogenet Cell Genet 53:137–139
Denekamp DS, Murnane MJ (1985) Human thiol cathepsin activities are decreased in chromosome 11 deletion cell lines. Am J Hum Genet 37:A229
Evans GA, Wahl GM (1987) Cosmid vectors for genomic walking and rapid restriction mapping. Methods Enzymol 152:604–610
Fan YS, Byers MG, Eddy RL, Joseph L, Sukathme V, Chan SJ, Shows TB (1989) Cathepsin L (CTSL) is located in the chromosome 9q21-q22 region; a related sequence is located on chromosome 10. Cytogenet Cell Genet 51:996
Fong D, Calhoun DH, Hsieh WT, Lee B, Wells RD (1986) Isolation of a cDNA clone for the human lysosomal proteinase cathepsin B. Proc Natl Acad Sci USA 83:2909–2913
Fong D, Smith DI, Hsieh WT (1991) The human kininogen gene (KNG) mapped to chromosome 3q26-qter by analysis of somatic cell hybrids using polymerase chain reaction. Hum Genet 17:189–192
Hanson RD, Hohn PA, Popescu NC, Ley TJ (1990) A cluster of hematopoietic serine protease genes is found on the same chromosomal band as the human α/δ T-cell receptor locus. Proc Natl Acad Sci USA 87:960–963
Hsieh WT, Barker PE, Smith M, Fong D (1990) A TaqI DNA polymorphism in the human cathepsin B gene (CTSB). Nucleic Acids Res 18:3430
Hsieh WT, Fong D, Sloane BF, Golembieski W, Smith DI (1991) Mapping of the gene for human cysteine proteinase inhibitor stefin A, STF 1, to chromosome 3cen-q21. Genomics 9:207–209
Lichter P, Chang Tang CJ, Call K, Hermanson G, Evans GA, Housman D, Ward DC (1990) High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69
Lux SE, Tse WT, Menninger JC, John KM, Harris P, Shalev O, Chilcote RR, Marchesi SL, Watkins PC, Bennett V, McIntosh S, Collins FS, Francke U, Ward DC, Forget BG (1990) Hereditary spherocytosis associated with deletion of human erythrocyte ankyrin gene on chromosome 8. Nature 345:736–739
Moin K, Rozhin J, McKernan TB, Sanders VJ, Fong D, Honn KV, Sloane BF (1989) Enhanced levels of cathepsin B mRNA in murine tumors. FEBS Lett 244:61–64
Qin S, Nakai H, Byers MG, Eddy RL, Haley LL, Henry WM, Wang X, Watkins PC, Chirgwin JM, Shows TB (1987) Mapping FSHB, CAT and CTSB to specific sites on 11p. Cytogenet Cell Genet 46:628
Sloane BF (1990) Cathepsin B and cystatins: evidence for a role in cancer progression. Semin Cancer Biol 1:137–152
Wang X, Chan SJ, Eddy RL, Byers MG, Fukushima Y, Henry WM, Haley LL, Stei'ner DF, Shows TB (1987) Chromosome assignment of cathepsin B (CTSB) to 8p22 and cathepsin H (CTSH) to 15q24-q25. Cytogenet Cell Genet 46:710–711
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Fong, D., Man-Ying Chan, M., Hsieh, WT. et al. Confirmation of the human cathepsin B gene (CTSB) assignment to chromosome 8. Hum Genet 89, 10–12 (1992). https://doi.org/10.1007/BF00207033
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DOI: https://doi.org/10.1007/BF00207033