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Indole compounds released by the ectendomycorrhizal fungal strain MrgX isolated from a pine nursery

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Abstract

The production and metabolism of indole compounds in pure cultures of the ectendomycorrhizal strain MrgX, a common symbiont of Scots pine in forest nurseries, were investigated. Different indole compounds produced by this fungus were purified and identified by thin-layer chromatography, high-performance liquid chromatography and mass spectrometry. Indole-3-acetic acid (IAA) and indole-3-carboxylic acid were the most abundant. Although MrgX is able to synthesize IAA when cultivated on a medium without tryptophan, much higher IAA production was obtained when 1 mM tryptophan was added. Buffering of the medium at pH 5.8 was shown to be essential for IAA accumulation in the culture filtrate. In vitro IAA-synthesizing activity of the enzymes extracted from the mycelia of MrgX was also maximal when mycelia were grown in a buffered, tryptophan-supplemented medium. The hydrogen ion concentration strongly affected in vivo activity of IAA-synthesizing enzymes. This activity was rather weak at acid pH and was stimulated by increase in pH up to 8.5. These results and their possible significance for ectendo-mycorrhizal symbiosis are discussed with reference to the hormonal metabolism of ectomycorrhizal fungi and ectomycorrhizae.

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Author notes

  1. M. Rudawska

    Present address: Institute of Dendrology, Polish Academy of Sciences, 62-035, Kórnik, Poland

Authors and Affiliations

  1. Unité d'Ecologie Microbienne du Sol Associée au Centre National de la Recherche Scientifique (Bât. 405), 43, Boulevard du 11 Novembre, F-69622, Villeurbanne Cedex, France

    M. Rudawska & G. Gay

  2. Laboratoire de Mycochimie, Université Claude Bernard Lyon 1, 43, Boulevard du 11 Novembre, F-69622, Villeurbanne Cedex, France

    J. Bernillon

Authors
  1. M. Rudawska
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  2. J. Bernillon
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  3. G. Gay
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Rudawska, M., Bernillon, J. & Gay, G. Indole compounds released by the ectendomycorrhizal fungal strain MrgX isolated from a pine nursery. Mycorrhiza 2, 17–23 (1992). https://doi.org/10.1007/BF00206279

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