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Kinetic correlation of recovery from photoinhibition and zeaxanthin epoxidation

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Abstract

The generation of non-photochemical fluorescence quenching under photoinhibitory illumination and its relaxation under subsequent low light illumination in leaves from intermittent-light-grown pea (Pisum sativum L.) plants (IML-plants) has been investigated. In parallel, we studied (i) the activity of the xanthophyll cycle with emphasis on zeaxanthin formation and reconversion to violaxanthin and (ii) the degradation rate of D1 protein. In comparison to control plants grown in continuous light, IML-plants were much more susceptible to photoinhibition as determined from the increase of slowly (halftimes > 20 min) relaxing quenching (qI) of variable chlorophyll fluorescence. The relaxation (recovery) kinetics of qI (under weak light) in both types of plant depended on the photon flux density, temperature and duration of pre-illumination. The recovery time generally increased with an increasing degree of qI. In IML-plants, relaxation of qI was kinetically closely related to the epoxidation of zeaxanthin. At high degrees of photosystem II inhibition the kinetics resembled those of D1 degradation. The results are discussed in terms of the mechanisms of photosystem II inactivation in vivo.

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Abbreviations

A:

antheraxanthin

CAB protein:

chlorophyll a/b-binding protein

Chl:

chlorophyll

CL:

continuous light

FM :

maximum fluorescence

FV :

variable fluorescence

IML:

intermittent light

PFD:

photon flux density

qE:

energy-dependent fluorescence quenching

qI:

photoinhibitory fluorescence quenching

SM:

streptomycin

V:

violaxanthin

Z:

zeaxanthin

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Authors and Affiliations

  1. Institut für Biochemie der Pflanzen, Heinrich-Heine-Universtät Düsseldorf, Universitätsstrasse 1, D-40225, Düsseldorf, Germany

    Peter Jahns & Birgit Miehe

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  1. Peter Jahns
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  2. Birgit Miehe
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Additional information

We wish to thank Dr. G.H. Krause (Institute of Plant Biochemistry, University of Düsseldorf, Germany) for helpful discussions and critical reading of the manuscript and Drs. D. Godde (Dept. of Plant Biochemistry, University of Bochum, Germany) and B. Andersson (Dept. of Biochemistry, University of Stockholm, Sweden) for kindly providing us with antibodies. This work was supported by the Deutsche Forschungsgemeinschaft (SFB 189).

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Jahns, P., Miehe, B. Kinetic correlation of recovery from photoinhibition and zeaxanthin epoxidation. Planta 198, 202–210 (1996). https://doi.org/10.1007/BF00206245

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  • DOI: https://doi.org/10.1007/BF00206245

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