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Immunomodulating activity in supernatants from EBV immortalized lymphocytes

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Summary

Our earlier work has demonstrated that EBV immortalized B lymphocytes are involved in a factor dependent autostimulatory cycle. Soluble growth stimulating activity was released into culture supernatants by these growing B cells. Growth enhancing (GE) media from B lymphocyte lines, immortalized by EBV infection, contained soluble factor(s) which modulated the Con A response of normal human mononuclear cells. Conditioned media from these lines affected the Con A response in a biphasic manner, stimulating the blastogenic response at lower concentrations, while inhibiting at higher concentrations. At stimulatory concentrations, the blastogenic response to Con A began earlier than in controls and was markedly enhanced by day 2. GE media reduced the initial response of purified B cells to pokeweed mitogen. GE media did not support growth of IL-2 dependent cells. GE media from some EBV-carrying B cell lines had measurable IL-1 activity in the mouse thymocyte PHA response. GE media from LPS stimulated B lymphocyte lines produced significant IL-1-like effects on stimulated mouse thymocytes. These results suggested that these B cell lines may produce IL-1-like factors that cooperate in T cell responses. The possibility that such factors may play a role in B lymphocyte transformation by EBV is discussed.

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Authors and Affiliations

  1. Department of Surgery, Brigham & Women's Hospital, Harvard Medical School, 02115, Boston, Ma., USA

    Beverly A. Blazar, Linda M. Sutton & Marshall Strome

Authors
  1. Beverly A. Blazar
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  2. Linda M. Sutton
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  3. Marshall Strome
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Supported by grants from the Concern Foundation and the Brigham Surgical Foundation

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Blazar, B.A., Sutton, L.M. & Strome, M. Immunomodulating activity in supernatants from EBV immortalized lymphocytes. Cancer Immunol Immunother 22, 62–67 (1986). https://doi.org/10.1007/BF00205718

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  • DOI: https://doi.org/10.1007/BF00205718

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