Summary
Short-term exposure of L1210 Ha leukemia to DTIC in vivo or in vitro resulted in the generation of leukemic cells that were moderately immunogenic for histocompatible (BALB/C × DBA/2)F1 (CD2F1) mice. In vivo treatment was carried out in the peritoneal cavity of CD2F1 host for 8–36 h. In vitro experiments were performed in glass vessels, in which tumor cells were incubated with DTIC for 2 h at 37° C. The in vitro generation of immunogenic leukemia was conditioned by the presence of mouse liver microsomes capable of producing metabolic transformation of DTIC. It follows that the increase of tumor cell immunogenicity produced in vitro and possibly in vivo by DTIC is due to (a) metabolic product (s) that has (have) not yet been identified. Somatic mutation, selection, viral activation, or other mechanisms could be responsible for the DTIC effect. The present studies suggest that similar in vivo or in vitro techniques could be used to obtain human tumor cells with higher immunogenicity.
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Abbreviations
- AIC:
-
5-aminoimidazole-4-carboxamide
- BCNU:
-
1,3-bis-chloroethyl nitrosourea
- Cy:
-
of cyclophosphamide
- DMITI:
-
drug-mediated increase tumor immunogenicity
- DTIC:
-
5(3,3′-dimethyl-1-triazeno)-imidazole-4-carboxamide
- MLP:
-
mouse liver preparation
- PB:
-
phenobarbital
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Contessa, A.R., Giampietri, A., Bonmassar, A. et al. Increased immunogenicity of L1210 leukemia following short-term exposure to 5(3,3′-dimethyl-1-triazeno)-imidazole-4-carboxamide (DTIC) in vivo or in vitro. Cancer Immunol Immunother 7, 71–76 (1979). https://doi.org/10.1007/BF00205327
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DOI: https://doi.org/10.1007/BF00205327