Abstract
The gene encoding the Kell blood group polypeptide has been localized to chromosome 7q33-35 by in situ hybridization using a biotinylated 1.1-kb DNA fragment containing the 3′ half of the human cDNA. This assignment is in accord with genetic localization using antigenic variation as a marker, and strongly suggests that Kell antigenic determinants are part of the polypeptide chain rather than the associated sugar molecules.
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Barker PE, Ruddle FH, Royer HD, Acuto O, Reinherz EL (1984) Chromosomal location of human T cell receptor gene Ti beta. Science 226:348–349
Boyd E, Theriault A, Goddard JP, Kalaitsidaki M, Spathas DH, Connor JM (1989) Chromosomal assignment of a glutamic acid transfer RNA (tRNAGlu) gene to 1p36. Hum Genet 81:153–156
Carter NP, Ferguson-Smith MA, Perryman MT, Telenius H, Pelmear AH, Leversha MA, Glancy MT, Wood SL, Cook K, Dyson HM, Ferguson-Smith ME, Willatt LR (1992) Reverse chromosome painting: a method for the rapid analysis of aberrant chromosomes in clinical cytogenetics. J Med Genet 29:299–307
Croce C, Koprowski HM (1974) Somatic cell hybrids between mouse peritoneal macrophages and SV-40 transformed human cells. 1. Positive control of the transformed phenotype by the human chromosome 7 carrying SV-40 genome. J Exp Med 140:1221–1229
D'Adamio L, Shipp MA, Masteller EL, Reinherz EL (1989) Organization of the gene encoding common acute lymphoblastic leukemia antigen (neutral endopeptidase 24. 11): multiple miniexons and separate 5′ untranslated regions. Proc Natl Acad Sci USA 86:7103–7107
Edwards YH, Parkar M, Povey S, West LF, Parrington JM, Solomon E (1985) Human myosin heavy chain genes assigned to chromosome 17 using a cDNA clone as a probe. Ann Hum Genet 49:101–109
Garson JA, Berghe JAVan den, Kemshead JT (1987) Novel nonisotopic in situ hybridization technique detects small (1 kb) unique sequences in routinely G-banded human chromosomes: fine mapping of N-myc and beta-NGF genes. Nucleic Acids Res 15:4761–4770
Heisterkamp N, Groffen J, Stephenson JR, Spurr NK, Goodfellow PN, Solomon E, Carritt B, Bodmer WF (1982) Chromosomal localization of human cellular homologues to two viral oncogenes. Nature 299:747–749
Hobart MJ, Rabbitts TH, Goodfellow PN, Solomon E, Chambers S, Spurr N, Povey S (1981) Immunoglobulin heavy chain genes in humans are located on chromosome 14. Ann Hum Genet 45:331–335
Jones EA, Goodfellow PN, Kennett RH, Bodmer WF (1976) The independent expression of HLA and β2 microglobulin on mouse-human hybrids. Somatic Cell Genet 2:483–496
Kielty CM, Povey S, Hopkinson DA (1982) Regulation of expression of liver-specific enzymes. III. Further analysis of a series of rat hepatoma × human somatic cell hybrids. Ann Hum Genet 46:307–327
Lee S, Zambas ED, Marsh WL, Redman CM (1991) Molecular cloning and primary structure of Kell blood group protein. Proc Natl Acad Sci USA 88:6353–6357
Marsh WL, Redman CM (1990) The Kell blood group system: a review. Transfusion 30:158–167
Nabholz M, Miggiano V, Bodmer WF (1969) Genetic analysis with human-mouse somatic cell hybrids. Nature 223:358–363
Palumbo AP, Isobe M, Huebner K, Shane S, Rovera G, Demuth D, Curtis PJ, Ballantine M, Croce CM, Showe LC (1986) Chromosomal localization of a human band 3-like gene to region 7q35→7q36. Am J Hum Genet 39:307–316
Phillips IR, Shephard EA, Povey S, Davis MB, Kelsey G, Monteiro M, West LF, Cowell F (1985) A cytochrome P-450 gene family mapped to human chromosome 19. Ann Hum Genet 49:267–274
Pinkel D, Straume T, Gray JW (1986) Cytogenetic analysis using quantitative, high sensitivity, fluorescence hybridization. Proc Natl Acad Sci USA 83:2934–2938
Povey S, Jeremiah SJ, Barker RF, Hopkinson DA, Robson EB, Cook PJL, Solomon E, Bobrow M, Carritt B, Buckton KE (1980) Assignment of the human locus determining phosphoglycolate phosphatase (PGP) to chromosome 16. Ann Hum Genet 43:241–248
Purohit KR, Weber JL, Ward LJ, Keats BJB (1992) The Kell blood group locus is close to the cystic fibrosis locus on chromosome 7. Hum Genet 89:457–458
Redman CM, Avellino G, Pfeffer SR, Mukherjee TK, Nichols M, Rubinstein P, Marsh WL (1986) Kell blood group antigens are part of a 93000-dalton red cell membrane protein. J Biol Chem 261:9521–9525
Saiki RK, Gelfand DH, Stoffel S, Scharf SJ, Higuchi R, Horn GT, Mullis KB, Erlich HA (1988) Primer-directed enzymatic amplification of DNA with a thermostable DNA polymerase. Science 239:487–491
Solomon E, Bobrow M, Goodfellow PN, Bodmer WF, Swallow DM, Povey S, Noel B (1976) Human gene mapping using an X/autosome translocation. Somatic Cell Genet 2:125–140
Van Heyningen V, Bobrow L, Bodmer WF, Gardiner SE, Povey S, Hopkinson DA (1975) Chromosome assignment of some human loci: mitochondrial malate dehydrogenase to 7, mannose phosphate isomerase and pyruvate kinase to 15, and probably esterase D to 13. Ann Hum Genet 38:295–303
Zelinski T, Coghlan G, Myal Y, Shiu RPC, Philipps S, White L, Lewis M (1991a) Genetic linkage between the Kell blood group system and prolactin-inducible protein loci: provisional assignment of KEL to chromosome 7. Ann Hum Genet 55:137–140
Zelinski TA, Coghlan GE, Myal Y, White LJ, Phillips SE (1991b) Assignment of the Kell blood group locus to chromosome 7q. Cytogenet Cell Genet 58:1927
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Murphy, M.T., Morrison, N., Miles, J.S. et al. Regional chromosomal assignment of the Kell blood group locus (KEL) to chromosome 7q33-q35 by fluorescence in situ hybridization: evidence for the polypeptide nature of antigenic variation. Hum Genet 91, 585–588 (1993). https://doi.org/10.1007/BF00205085
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DOI: https://doi.org/10.1007/BF00205085