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Involvement of abscisic acid in photosynthetic process in Hordeum vulgare L. during salinity stress

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Abstract

In Hordeum vulgare L. plants, NaCl stress imposed through the root medium for a period of 8 days decreased the rate of CO2 assimilation, the chlorophyll and protein leaf content, and the activity of ribulose-1,5-bisphosphate carboxylase. The activity of phosphoenolpyruvate carboxylase was twofold over the control. Pretreatment with abscisic acid (ABA) for 3 days before salinization diminished the inhibitory effect of NaCl on the rate of CO2 fixation. The leaf Na+ and Cl− content decreased in ABA-pretreated plants. Both ABA and NaCl treatments led to an increase in the endogenous level of ABA in the plant leaves. Patterns of total proteins extracted from the leaves of control or ABA- and salt-treated plants were compared. Both ABA and NaCl induced marked quantitative and qualitative changes in the polypeptide profiles concerning mainly the proteins with approximately equal mobility. The results are discussed in terms of a possible role of ABA in increasing the salt tolerance when ABA is applied to the plants for a short period before exposure to salinity stress, thus improving the invulnerability to unfavorable conditions.

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Abbreviations

RuBPC:

ribulose-1,5-bisphosphate carboxylase

PSII:

photosystem II

ABA:

abscisic acid

PEPC:

phosphoenolpyruvate carboxylase

DTTr:

dithiothreitol

BSA:

bovine serum albumin

ELISA:

enzyme-linked immunosorbent assay

SDS:

sodium dodecyl sulfate

PAGEr:

polyacrylamide gel electrophoresis

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Authors and Affiliations

  1. Institute of Plant Physiology, Bulgarian Academy of Sciences, 1113, Sofia, Bulgaria

    Losanka P. Popova, Zhivka G. Stoinova & Liliana T. Maslenkova

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  1. Losanka P. Popova
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  2. Zhivka G. Stoinova
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  3. Liliana T. Maslenkova
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Popova, L.P., Stoinova, Z.G. & Maslenkova, L.T. Involvement of abscisic acid in photosynthetic process in Hordeum vulgare L. during salinity stress. J Plant Growth Regul 14, 211–218 (1995). https://doi.org/10.1007/BF00204914

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  • DOI: https://doi.org/10.1007/BF00204914

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