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Zum enzymatisch-immunochemischen Nachweis des Haschischkonsums und seiner dünnschichtchromatographischen Absicherung

Detection of cannabinoids in blood and urine by EMIT confirmation by TLC

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Summary

The EMIT cannabinoid assay was used for screening blood and urine after smoking tetrahydrocannabinol (THC; 10 mg) or ingestion of THC (30 mg). Cannabinoid levels in urine remain detectable up to 1 week. Confirmation was done by adsorption of the THC carboxylic acid onto a C18 extraction column and elution with acetone and TLC. The method is simple and sensitive and is applicable with common laboratory equipment. The detection limit is 10 ng/ml, using 10 ml urine.

Zusammenfassung

Mit Hilfe des Enzymimmunoassay (EMIT-dau-Syva) lassen sich Cannabinoide in Blut und Urin nach inhalativer (10 mg THC) oder oraler (30 mg THC) Aufnahme nachweisen. Die Kurvenverläufe werden mitgeteilt. Im Urin waren erhöhte Spiegel bis zu einer Woche nachweisbar. Eine Absicherung der EMIT-Befunde ist mittels Dünnschichtchromatographie nach absorptiver Anreicherung an einer C18-Extraktionssäule möglich. Nachweisgrenze ist 10 ng THC-Carbonsäure/ml bei Einsatz von 10 ml Urin.

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  1. Institut für Rechtsmedizin, Frauenlobstr. 7a, D-8000, München 2, Bundesrepublik Deutschland

    L. von Meyer

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  1. L. von Meyer
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von Meyer, L. Zum enzymatisch-immunochemischen Nachweis des Haschischkonsums und seiner dünnschichtchromatographischen Absicherung. Z Rechtsmed 94, 219–225 (1985). https://doi.org/10.1007/BF00204086

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  • DOI: https://doi.org/10.1007/BF00204086

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