Abstract
The serum kinetics of preS1 and preS2 antigens has been evaluated in 38 serial samples from eight patients with chronic active (CAH) or chronic persistent (CPH) hepatitis, followed for 2–7 years (mean 4.4 years) in whom liver biopsy was performed at intervals, and in 46 samples from ten asymptomatic HBsAg carriers followed for 4–5 years (mean 4.6 years). Four patterns of preS behaviour have been observed: (1) persistently positive preS1 and preS2; (2) disappearance of preS2; (3) disappearance of both preS1 and preS2; and (4) persistently negative preS1 and preS2. Pattern 4 has been observed exclusively among healthy carriers, while seven out of eight chronic patients exhibited either pattern 1 or 2. Among the chronic patients, preS2 disappearance was accompanied or followed by alanine aminotransferase (ALT) normalization. The correlation of preS antigens with conventional viral replication markers showed that 100% of hepatitis B virus (HBV)-DNA-positive and 86.6% of HBeAg-positive sera were preS1/preS2 positive, while 61% of HBV-DNA-negative and 64% of HBeAg-negative sera were preS1/preS2 negative. Our data suggest that continuous monitoring of preS antigens in follow-up sera will allow for an improved prognostic evaluation of chronic HBV infection.
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Barnaba V, Franco A, Alberti A, Benvenuto R, Balsano F (1989) Recognition of hepatitis B virus envelope proteins by liver-infiltrating T lymphocytes in chronic HBV infection. J Immunol 143:2650–2655
Budkowska A, Dubreuil P, Gerlich WH, Lazizi Y, Pillot J (1988a) Occurrence of preS1 antigen in viremic and nonviremic carriers of hepatitis B surface antigen. J Med Virol 26:217–225
Budkowska A, Dubreuil P, Pillot J (1988b) Prognostic value of preS2 epitopes of hepatitis B virus and anti-preS2 response evaluated by monoclonal assay. In: Zuckerman AJ (ed) Viral hepatitis and liver disease. Alan R. Liss, New York, pp 287–289
Czaja AJ, Wolf AM, Baggenstoss AH (1981) Laboratory assesments of severe chronic active liver disease (CALD). Correlation of serum transaminases and gammaglobulin levels with histologic features. Gastroenterology 80:687–692
Deepen R, Heermann KH, Uy A, Thomssen R, Gerlich WH (1990) Assay of preS epitopes and PreS 1 antibody in hepatitis B virus carriers and immune persons. Med Microbiol Immunol 179:49–60
Delfini C, Colloca S, Taliani G, Mazzotta F, D'agata A, Buonamici C, Stroffolini T, Carloni G (1989) Clearance of hepatitis B virus DNA and preS surface antigens in patients with markers of acute viral replication. J Med Virol 28:169–175
Ferrari C, Mondelli MV, Penna A, Fiaccadori F, Chisari FV (1987) Functional characterization of cloned intrahepatic hepatitis B virus nucleoprotein-specific helper T cell lines. J Immunol 139:539–544
Gerken G, Manns M, Gerlich WH, Hess G, Meyer Zum Bschenfelde KH (1987) preS encoded surface proteins in relation to the major viral surface antigen in acute hepatitis B virus infection. Gastroenterology 92:1864–1868
Gerken G, Manns M, Gerlich WH, Meyer Zum Buschenfelde KH (1989) Immune blot analysis of viral surface proteins in serum and liver of patients with chronic hepatitis B virus infection. J Med Virol 29:261–265
Hadziyannis S, Raimondo G, Papaioannou C, Anastassakos C, Wong D, Sninsky J, Shafritz D (1988) Expression of preS gene-encoded proteins in liver and serum during chronic hepatitis B virus infection in comparison with other markers of active virus replication. In: Zuckerman AJ (ed) Viral hepatitis and liver disease. Alan R. Liss, New York, pp 363–364
Heermann KH, Goldman U, Schwartz W, Seyffarth T, Baumgarten H, Gerlich WH (1984) Large surface proteins of hepatitis B virus containing the preS sequences. J Virol 52:396–402
Heermann KH, Kruse F, Seifer M, Gerlich WH (1987) Immunogenicity of the gene S and preS domains in hepatitis B virions and HBsAg filaments. Intervirology 28:14–25
Heermann KH, Waldeck F, Gerlich WH (1988) Interaction between native human serum and the preS2 domain of hepatitis B virus surface antigen. In: Zuckermann AJ (ed) Viral hepatitis and liver disease. Alan R Liss, New York, pp 697–700
Hu PS, Carithers RL Jr, Fiorenza V, Peterson DL (1987) Quantitative studies of the hepatitis B viral preS proteins: lack of correlation with the HBeAg status. J Virol Methods 16:97–114
Kuijpers L, Koens M, Murray-Lyon I, Coleman JC, Karayiannis P, Thomas HC, Zanetti A, Cargnel A, Harrison TJ, Zuckerman AJ (1989) preS proteins in Hepatitis B. J Med Virol 28:47–51
Kurai K, Iino S, Koike K, Endo Y, Oka H (1989) Serum titers of pre-S(2) antigen in patients with acute and chronic type B hepatitis: relation to serum aminotransferase activity and other hepatitis B virus markers. Hepatology 9:175–179
Machida A, Kishimoto S, Ohnuma H, Baba K, Ho Y, Miyamoto H, Funatsu G, Oda K, Usuda S, Tagani S, Nakamura T, Miyakawa Y, Mayumi M (1984) A polypeptide containing 55 amino acid residues B virus deoxyribonucleic acid bears the receptor for polymerized human as well as chimpanzee albumins. Gastroenterology 86:910–918
Milich DR, Thornton GB, Neurath AR, Kent SB, Michel ML, Thiollais P, Chisari FV (1985) Enhanced immunogenicity of the preS region of hepatitis B surface antigen. Science 228:1195–1199
Milich DR, McLachlan A, Chisari FV, Kent SBH, Thornton GB (1986) Immune response to the preS(1) region of the hepatitis B surface antigen (HBsAg): a preS(1)-specific T cell response can bypass non-responsiveness to the preS(2) and S regions of HBsAg. J Immunol 137:315–322
Mondelli MV, Naumov N, Eddleston ALWF (1984) The immunopathogenesis of liver damage in chronic hepatitis B virus infection. In: Chisari FV (ed) Advances in hepatitis research. Masson, New York, pp 144–165
Morace G, Von Der Helm K, Jilg W, Deinhardt F (1985) Detection of hepatitis B virus DNA in serum by a rapid filtration-hybridization assay. J Virol Methods 12:235–242
Neurath AR, Kent SBM, Strick NB (1984) Location and chemical synthesis of a preS gene coded immuno dominant epitope of hepatitis B virus. Science 224:392–395
Neurath AR, Kent SBH, Strick N, Parker N (1986) Identification and chemical synthesis of a host cell receptor binding site on hepatitis B virus. Cell 46:429–436
Pontisso P, Petit MA, Bankowski MJ, Peeples ME (1989a) Human liver plasma membranes contain receptors for the hepatitis B virus pre-S1 region and, via polymerized human serum albumin, for the pre-S2 region. J Virol 63:1981–1988
Pontisso P, Ruvoletto MG, Gerlich WH, Heermann KH, Bardini R, Alberti A (1989b) Identification of an attachment site for human liver plasma membranes on hepatitis B virus particles. Virology 173:522–530
Stibbe W, Gerlich WH (1983) Structural relationship between minor and major proteins of hepatitis B surface antigen. J Virology 40:626–628
Thomas HC, Pignatelli M, Goodal A, Waters J, Karyiannis P, Brown D (1984) Immunologic mechanisms of cell lysis in hepatitis B virus infections. In: Vyas G, Dienstag JL, Hoofnagle J (eds) Viral hepatitis and liver disease. Grune & Stratton, Orlando, pp 167–177
Tiollais P, Charnay P, Vyias GN (1981) Biology of hepatitis B virus. Science 213:406–411
Van Ditzhuijsen TJM, Kuijpers LPC, Koens RJ, Rijntjens PJM, Van Loon AM, Yap SH (1990) Hepatitis B pre-S1 and pre-S2 proteins: clinical significance and relation to hepatitis B virus DNA. J Med Virol 32:87–91
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Taliani, G., Rapicetta, M., Francisci, D. et al. Correlation of preS antigens and clinical status during chronic hepatitis B virus infection. Med Microbiol Immunol 180, 239–248 (1991). https://doi.org/10.1007/BF00202558
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DOI: https://doi.org/10.1007/BF00202558