Abstract
The effect of various strains of influenza virus on polymorphonuclear leucocyte (PMNL) function were studied by chemiluminescence (CL) and bacterial killing assays. All virus strains induced PMNL CL and peak CL correlated with haemagglutination (HA) but not neuraminidase (NA) activity of virus pools. Heat-treatment of virus pools generally had little effect on HA activity or ability to generate a PMN CL response but almost completely destroyed NA activity. Exposure of PMNL to each of the six virus strains resulted in loss of surface-associated sialic acid and a marked depression in both zymosan-induced PMNL CL and PMNL bactericidal capacity. However, there was no correlation between the degree of PMNL functional impairment and virus NA activity and, furthermore, heat treatment of virus pools removed NA activity but generally had little effect on their ability to reduce PMNL function. NA does not apear to play a primary role in impairment of PMNL function by influenza virus.
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References
Abramson JS, Mills EL (1988) Depression of neutrophil function induced by viruses and its role in secondary microbial infections. Rev Infect Dis 10:326–341
Abramson JS, Lewis JC, Lyles DS, Heller KA, Mills EL, Bass DA (1982a) Inhibition of neutrophil lysosome-phagosome fusion associated with influenza virus infection in vitro. Role in depressed bactericidal activity. J Clin Invest 69:1393–1397
Abramson JS, Mills EL, Giebink GS, Quie PG (1982b) Depression of monocytes and polymorphonuclear leucocyte oxidative metabolism and bactericidal capacity by influenza A virus. Infect Immun 35:350–355
Abramson JS, Wiegand GL, Lyles DS (1985) Neuraminidase is not the cause of influenza virus induced neutrophil dysfunction. J Clin Microbiol 22:129–131
Busse WW, Sosman JM (1981) Altered luminol-dependent granulocyte chemiluminescence during an in vitro incubation with an influenza vaccine. Am Rev Respir Dis 123:654–658
Caldwell SE, Cassidy LF, Abramson JS (1988) Alterations in the cell protein phosphorylation in human neutrophils exposed to influenza A virus. A possible mechanism for depressed cellular end-stage functions. J Immunol 140:3560–3567
Hastings MJG, Petricevic I, Williams AJ, Cole PJ, Easmon CSF (1982) The effect of culture media on the production and measurement of luminoldependent chemiluminescence. Br J Exp Path 63:147–153
Hayakawa T, Suzuki K, Suzuki S, Andrews PC, Babior BM (1986) A possible role of protein phosphorylation in the activation of the respiratory burst in human neutrophils. Evidence from studies with cells from patients with chronic granulomatous disease. J Biol Chem 269:9109
Henricks PAJ, van der Tol ME, Venhoef J (1982) Partial removal of sialic acid enhances phagocytosis and the generation of superoxide and chemiluminescence by PMN. J Immunol 129:745–750
Henricks PAJ, van der Tol ME, Venhoef J (1985) Interactions between human polymorphonuclear leucocytes and influenza virus. Scand J Immunol 22:721–725
Hoover RL, Briggs RT, Karnovsky (1978) The adhesive interaction between polymorphonuclear leucocytes and endothelial cells in vitro. Cell 14:423–428
Lowry OH, Rosenbrough NJ, Farr AL, Romdall RJ (1951) Protein measurement with the folin phenol reagent. J Biol Chem 193:265–275
Mills EL, Ossenkopp YD, Verbugh HA, Venhoef J (1981) Initiation of the respiratory burst of human neutrophils by influenza viruses. Infect Immun 32:1200–1205
Peterhans E (1980) Chemiluminescence: An early event in the interaction of Sendai and influenza viruses with mouse spleen cells. I. The role of the envelope glycoproteins in the stimulation of chemiluminescence. Virology 105:445–455
Segal AW, Heyworth PG, Cockcroft S, Barrowman MM (1985) Stimulated neutrophils from patients with autosomal recessive chronic granulomatous disease fail to phosphorylate a Mr-44,000 protein. Nature 316:547–549
Shult PA, Dick EC, Joiner KA, Busse WW (1985) Role of serum in stimulation of polymorphonuclear leukocyte, luminol-dependent chemiluminescence by influenza A. Am Rev Respir Dis 131:267–272
Suzuki H, Kurita T, Kakinuma K (1982) Effects of neuraminidase on O2 consumption and release of O2 and H2O2 from phagocytosing human polymorphonuclear leucocytes. Blood 60:446–453
Tsan MF, McIntyre PA (1976) The requirement for membrane sialic acid in the stimulation of superoxide production during phagocytosis by human polymorphonuclear leucocytes. J Exp Med 143:1308–1316
Warren L (1959) The thiobarbituric acid assay of sialic acid. J Biol Chem 234:1971–1975
Weiss L (1965) Studies on cell deformability. J Cell Biol 26:735–739
Who Expert Committee on Respiratory Virus Diseases (1953) World Health Organization Technical Report Series No. 64. WHO, Geneva
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Ertürk, M., Jennings, R., Oxley, K.M. et al. Effect of influenza A on phagocytic cell function. Med Microbiol Immunol 178, 199–209 (1989). https://doi.org/10.1007/BF00202553
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DOI: https://doi.org/10.1007/BF00202553