Skip to main content
SpringerLink
Log in

An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13

  • Original Investigations
  • Published:
Human Genetics Aims and scope Submit manuscript

Abstract

Using vector-CENP-B box polymerase chain reaction (PCR) we isolated and cloned from a human chromosome 21-specific plasmid library, a 1 kb DNA sequence, named pαH21. In in situ hybridization experiments, pαH21 hybridized, under high stringency conditions, to the centromeric region of all the human, chimpanzee, gorilla and orangutan chromosomes. On human chromosomes pαH21 also identified non-centromeric sequences at 2q21 (locus D2F33S1) and 9q13 (locus D9F33S2). The possible derivation of these sequences from ancestral centromeres is discussed. Sequence analysis confirmed the alphoid nature of the whole pαH21 insert.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aleixandre C, Miller DA, Mitchell AR, Warburton DA, Gersen SL, Disteche C, Miller OJ (1987) p82H identifies sequences at every human centromere. Hum Genet 77:46–50

    Google Scholar 

  • Baldini A, Ward D (1991) In situ hybridization banding of human chromosomes with Alu-PCR products: a simultaneous karyotype for gene mapping studies. Genomics 9:770–774

    Google Scholar 

  • Baldini A, Smith DS, Rocchi M, Miller OJ, Miller DA (1989a) Cloning and analysis of 100 kbp of pericentromeric repetitive (alphoid) DNA from human chromosome 3. Am J Hum Genet 45:501

    Google Scholar 

  • Baldini A, Smith DS, Rocchi M, Miller OJ, Miller DA (1989b) A human alphoid DNA clone from the EcoRI dimeric family: genomic and internal organisation and chromosomal assignment. Genomics 5:822–828

    Google Scholar 

  • Baldini A, Miller DA, Miller OJ, Ryder OA, Mitchell AR (1991) A chimpanzee derived chromosome-specific alpha satellite DNA sequence conserved between chimpanzee and human. Chromosoma 100:156–161

    Google Scholar 

  • Baldini A, Archidiacono N, Carbone R, Bolino A, Shridhar V, Miller OJ, Miller DA, Ward DC, Rocchi M (1992) Isolation and comparative mapping of a human-chromosome 20-specific alpha-satellite DNA clone. Cytogenet Cell Genet 59:12–16

    Google Scholar 

  • Bellis M, Charlieu JP, Carter D, Orti R, Marcais B, Viegaspequignot E, Roizes G (1991) The use of the YAC technology to find new polymorphic markers to study the alzheimers-disease gene and the centromeric region of human chromosome-21. Am J Hum Genet 49:366

    Google Scholar 

  • Choo KH, Vissel B, Nagy A, Kalitsis P (1991) A survey of the genomic distribution of alpha satellite DNA on all human chromosomes, and derivation of a new consensus sequence. Nucleic Acids Res 19:1179–1182

    Google Scholar 

  • Collins C, Kuo WL, Segraves R, Fuscoe J, Pinkel D, Gray JW (1991) Construction and characterization of plasmid libraries enriched in sequences from single human chromosomes. Genomics 11:997–1006

    Google Scholar 

  • Devereux J, Haeberly P, Smithies O (1984) A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12:387–395

    CAS  PubMed  Google Scholar 

  • Durfy SJ, Willard HF (1990) Concerted evolution of primate alpha satellite DNA. Evidence for an ancestral sequence shared by gorilla and human X chromosome alpha satellite. J Mol Biol 216:555–566

    Google Scholar 

  • Goodman M, Koop BF, Czelusniak J, Fitch DHA, Tagle DA, Slightom JL (1989) Molecular phylogeny of the family of apes and humans. Genome 31:316–335

    Google Scholar 

  • Ijdo JW, Baldini A, Ward DC, Reeders ST, Wells RA (1991) Origin of human chromosome 2 — an ancestral telomere-telomere fusion. Proc Natl Acad Sci USA 88:9051–9055

    CAS  PubMed  Google Scholar 

  • ISCN (1985) An international system for human cytogenetic nomenclature. Harnden DG, Klinger HP (eds) Published in collaboration with Cytogenet Cell Genet. Karger, Basel New York

    Google Scholar 

  • Kurnit DM, Maio JJ (1973) Subnuclear distribution of DNA species in confluent and growing mammalian cells. Chromosoma 42:23–36

    Google Scholar 

  • Lichter P, Tang Chang C-J, Call K, Hermanson G, Evans GA, Housman D, Ward DC (1990) High-resolution mapping of human chromosome 11 by in situ hybridization with cosmid clones. Science 247:64–69

    Google Scholar 

  • Maio JJ (1971) DNA strand reassociation and polyribonucleotide binding in the African green monkey, Cercopithecus aethiops. J Mol Biol 56:579–595

    Google Scholar 

  • Manuelidis L (1978) Chromosomal location of complex and simple repeated human DNAs. Chromosoma 66:23–32

    Google Scholar 

  • Miller DA, Sharma D, Mitchell AR (1988) A human derived probe, p82H, hybridizes to the centromeres of gorilla, chimpanzee, and orangutan. Chromosoma 96:270–274

    Google Scholar 

  • Mitchell AR, Gosden Jr, Miller DA (1985) A cloned sequence, p82H, of the alphoid repeated DNA family found at the centromeres of all human chromosomes. Chromosoma 92:369–377

    Google Scholar 

  • Pluta AF, Cooke Ca, Earnshaw WC (1990) Structure of the human centromere at metaphase. Trends Biochem Sci 15:181–185

    Google Scholar 

  • Rattner JB (1991) The structure of the mammalian centromere. Bioessays 13:51–56

    CAS  PubMed  Google Scholar 

  • Rocchi M, Roncuzzi L, Santamaria R, Archidiacono N, Dente L, Romeo G (1986) Mapping through somatic cell hybrids and cDNA probes of protein C to chromosome 2, factor X to chromosome 13, and alpha 1-acid glycoprotein to chromosome 9. Hum Genet 74:30–33

    Google Scholar 

  • Rocchi M, Archidiacono N, Ward DC, Baldini A (1991) A human chromosome 9-specific alphoid DNA repeat spatially resolvable from satellite 3 DNA by fluorescent in situ hybridization. Genomics 9:517–523

    Google Scholar 

  • Rosenberg H, Singer M, Rosenberg M (1978) Highly reiterated sequences of Simian. Science 200:394–402

    Google Scholar 

  • Vissel B, Choo KH (1991) Four distinct alpha satellite subfamilies shared by human chromosomes 13, 14 and 21. Nucleic Acids Res 19:271–277

    Google Scholar 

  • Waye JS, Willard HF (1987) Nucleotide sequence heterogeneity of alpha satellite repetitive DNA: a survey of alphoid sequences from different human chromosomes. Nucleic Acids Res 15:7549–7569

    Google Scholar 

  • Waye JS, Mitchell AR, Willard HF (1988) Organization and genomic distribution of “82H” alpha satellite DNA. Evidence for a low-copy or single-copy alphoid domain located on human chromosome 14. Hum Genet 78:27–32

    Google Scholar 

  • Wilbur WJ, Lipman DJ (1983) Rapid similarity searches of nucleic acid and protein data banks. Proc Natl Acad Sci USA 80:726–730

    Google Scholar 

  • Willard HF, Waye JS (1987) Hierarchical order in chromosome-specific human alpha satellite DNA. Trends Genet 3:192–198

    Article  CAS  Google Scholar 

  • Wong AKC, Rattner JB (1988) Sequence organization and cytological localization of the minor satellite of mouse. Nucleic Acids Res 16:11645–11661

    CAS  PubMed  Google Scholar 

  • Yunis JJ, Prakash O (1982) The origin of man: a chromosomal pictorial legacy. Science 215:1525–1530

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Human Genetics, Yale University School of Medicine, 333 Cedar Street, 06510, New Haven, CT, USA

    Antonio Baldini, Thomas Ried, Keiko Ogura & David C. Ward

  2. CNR Institute of Molecular Genetics, Porto Conte Research and Training Laboratories, Alghero, Italy

    Antonio Baldini

  3. Imperial Cancer Research Fund, 44 Lincoln's Inn Fields, WC2A 3PX, London, UK

    Antonio Baldini

  4. Department of Molecular Biology and Genetics, Wayne State University School of Medicine, Detroit, Michigan, USA

    Viji Shridhar

  5. Istituto di Anatomia Umana Normale, Universita'di Modena, Modena, Italy

    Leonardo D'Aiuto

  6. Istituto di Genetica, Universita'di Bari, Bari, Italy

    Mariano Rocchi

Authors
  1. Antonio Baldini
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Thomas Ried
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Viji Shridhar
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Keiko Ogura
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Leonardo D'Aiuto
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Mariano Rocchi
    View author publications

    You can also search for this author in PubMed Google Scholar

  7. David C. Ward
    View author publications

    You can also search for this author in PubMed Google Scholar

Additional information

GenBank accession number, M64321

Rights and permissions

Reprints and permissions

About this article

Cite this article

Baldini, A., Ried, T., Shridhar, V. et al. An alphoid DNA sequence conserved in all human and great ape chromosomes: evidence for ancient centromeric sequences at human chromosomal regions 2q21 and 9q13. Hum Genet 90, 577–583 (1993). https://doi.org/10.1007/BF00202474

Download citation

  • Received:

  • Revised:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00202474

Keywords

Search

Navigation