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Pectin changes in samples containing poplar cambium and inner bark in relation to the seasonal cycle

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Abstract

Biochemical changes occurring during the transition between meristematic activity and rest were studied in samples containing cambial cells and their phloem derivatives from Populus x euramericana. Uronic acids represented around 9% of the cell-wall dry matter in spring and 7% in summer and winter. In contrast, a higher content of methylated galacturonic acids was observed during the rest period. The degree of esterification increased from 2% in spring to 35% in winter, indicating an important accumulation of acidic pectins during the active season although the cation content was always very low. Nuclear magnetic resonance spectroscopy of neutral polysaccharides solubilized with boiling water showed that in winter arabinans and xylans were the main carbohydrates. By contrast, in spring and in summer the xylans were very scarce, arabinans being the major neutral polysaccharide, indicating that important modifications occur during the autumn. Histochemical observations of material treated with hot water and EDTA confirmed the low relative pectin content during the rest period. Calcium ions, detected as antimonate salt were scarce. In the cambium, they were located mainly in cell junctions whereas in phloem derivatives these cations were distributed throughout the whole cell wall.

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Abbreviations

Araf:

arabinofuranose

COSY:

2 D homonuclear correlation spectroscopy

NMR:

nuclear magnetic resonance spectroscopy

NOESY:

2 D nuclear Overhauser effect spectroscopy

PATAg:

periodic acid thiosemicarbazide silver proteinate

PME:

pectin methylesterase

R wall:

radial wall

T wall:

tangential wall

Xylp:

xylopyranose

References

  • Aldington, S., McDougall, J., Fry, S.C. (1991) Structure activity relationships of biologically active oligosaccharides. Plant Cell Environ. 14, 625–636

    Google Scholar 

  • Aloni, R. (1992) The control of vascular differentiation. Int. J. Plant Sci. 153, S90-S92

    Google Scholar 

  • Baba, K., Sone, Y., Misaki, A., Shibuya, N., Hyashi, T., Itoh, T. (1992) Immunocytochemistry on the cell wall polysaccharides in the woody plants. In: Plant cell walls as biopolymers with physiological functions, pp. 327–331, Masuda, Y., ed. Yamada Science Foundation, Osaka

    Google Scholar 

  • Bax, A., Freeman, R. (1981) Investigation of complex networks of spin-spin coupling by two-dimensional NMR. J. Magn. Reson. 44, 542–561

    Google Scholar 

  • Benayoun, J., Catesson, A.M., Czaninski, Y. (1981) A cytochemical study of differentiation and breakdown of vessel end walls. Ann. Bot. 47, 687–698

    Google Scholar 

  • Bock, K., Thogersen, H. (1982) Nuclear magnetic resonance spectroscopy in the study of mono- and oligosaccharides. Annu. Reports on NMR Spectroscopy, vol. 13, pp. 1–49, Webb, G., ed. Academic Press, London

    Google Scholar 

  • Bock, K., Pedersen, C. (1983) Carbon-13 nuclear magnetic resonance spectroscopy of monosaccharides. Adv. Carbohydr. Chem. Biochem. 41, 47

    Google Scholar 

  • Bodenhausen, G., Kogler, H., Ernst, R.R. (1984) Selection of coherence transfer pathways in NMR pulse experiments. J. Magn. Reson. 58, 370–388

    Google Scholar 

  • Bolwell, G.P., Northcote, D.H. (1981) Control of hemicellulose and pectin synthesis during differentiation of vascular tissue in bean (Phaseolus vulgaris) callus and in bean hypocotyl. Planta 152, 225–233

    Google Scholar 

  • Bolwell, G.P., Northcote, D.H. (1983) Arabinan synthase and xylan synthase activities of Phaseolus vulgaris. Subcellular localization and possible mecanism of action. Biochem. J. 210, 497–507

    Google Scholar 

  • Bolwell, G.P., Dalessandro, G., Northcote, D.H. (1985) Decrease of polygalacturonic acid synthase during xylem differentiation in sycamore. Phytochemistry 24, 699–702

    Google Scholar 

  • Capmek, P., Toman, R., Kardosova, A., Rosik, J. (1983) Polysaccharides from the roots of the marsh mallow (Althaea officinalis L.) Carbohydr. Res. 117, 133–140

    Google Scholar 

  • Catesson, A.M. (1980) The vascular cambium. In: Control of shoot growth in trees, pp. 12–40, Little, C.H.A., ed. IUFRO Workshop Proceedings, Maritimes Forest Research Centre, Fredericton, NB, Canada

    Google Scholar 

  • Catesson, A.M. (1982) Cell wall architecture in the secondary sieve tubes of Acer and Populus. Ann. Bot. 49, 131–142

    Google Scholar 

  • Catesson, A.M. (1989) Specific characters of vessel primary walls during the early stages of wood differentiation. Biol. Cell, 67, 221–226

    Google Scholar 

  • Catesson, A.M. (1990) Cambial cytology and biochemistry. In: The vascular cambium, pp. 63–112, Iqbal, M., ed. Research Studies Press, J. Wiley and Sons Inc., Taunton Somerset

    Google Scholar 

  • Catesson, A.M., Roland, J.C. (1981) Sequential changes associated with cell wall formation and fusion in the vascular cambium. IAWA Bull. 2, 151–162

    Google Scholar 

  • Creber, G.T., Chaloner, W.G. (1990) Environmental influences on cambial activity. In: The vascular cambium, pp. 159–199, Iqbal, M., ed. Research Studies Press Ltd, J. Wiley and Sons Inc., Taunton Somerset

    Google Scholar 

  • Czaninski, Y., Monties, B., Roland, J.C., Catesson, A.M. (1982) Localisation de polysaccharides dans les parois lignifiées après extractions ménagées. Colloque Sciences et Industries du Bois, Grenoble 20–22 sept. 1982, 123–134

  • Dalessandro, G., Northcote, D.H. (1981) Increase of xylan synthetase activity during xylem differentiation of the vascular cambium of sycamore and poplar trees. Planta 151, 61–67

    Google Scholar 

  • Edashige, Y., Kakagawa, K., Ishii, T. (1992) Polysaccharides in cambial tissues of Cryptomeria japonica. In: Plant cell walls as biopolymers with physiological functions, pp. 361–36, Masuda, Y., ed. Yamada Science Foundation, Osaka

    Google Scholar 

  • Funada, R., Catesson, A.M. (1991) Partial cell wall lysis and the resumption of meristematic activity in Fraxinus excelsior cambium. IAWA Bull. N.S. 12, 439–444

    Google Scholar 

  • Goldberg, R., Morvan, C., Roland, J.C. (1986) Composition, properties and localization of pectins in young and mature cells of the mung bean hypocotyl. Plant Cell Physiol. 27, 419–429

    Google Scholar 

  • Goldberg, R., Morvan, C., Hervé du, Penhoat C., Michon, V. (1989) Structure and properties of acidic polysaccharides from mung bean hypocotyls. Plant Cell Physiol. 30, 163–173

    Google Scholar 

  • Goldberg, R., Pierron, M., Durand, L., Mutaftschiev, S. (1992) In vitro and in situ properties of cell wall pectinmethylesterases from mung bean hypocotyls. J. Exp. Bot. 43, 41–46

    Google Scholar 

  • Hervé du Penhoat, C., Michon, V., Goldberg, R. (1987) Development of arabinans and galactans during the maturation of hypocotyl cells of mung bean (Vigna radiata Wilezeck). Carbohydr. Res. 165, 31–34

    Google Scholar 

  • Kerr, T., Bailey, I.W. (1934) The cambium and its derivative tissues. X. Structure, optical properties and chemical composition of the so-called middle lamella. J. Arnold Arbor. 15, 327–349

    Google Scholar 

  • Lachaud, S. (1989) Participation of auxin and abscisic acid in the regulation of seasonal variations in cambial activity and xylogenesis. Trees 3, 125–137

    Google Scholar 

  • Little, C.H.A., Savidge, R.A. (1987) The role of plant growth regulators in forest tree cambial growth. Plant Growth Regulation 6, 137–169

    Google Scholar 

  • Mendonça-Previato, L., Gorin, P.A.J., Previato, J.O. (1979) Investigations on polysaccharide components of cells of Herpetomones samuelpessoai grown on various media. Biochemistry 18, 149–154

    Google Scholar 

  • Northcote, D.H. (1963) Changes in the cell walls of plants during differentiation. Symposia Soc. Exp. Biol. 17, 157–174

    Google Scholar 

  • Northcote, D.H. (1984) Control of cell wall assembly during differentiation. In: Structures, function and biosynthesis of plant cell walls, pp. 222–234, Dugger, W.M., Bartnicki-Garcia, S., eds. The American Society of Plant Physiologist Publ.

  • Northcote, D.H. (1985) Control of cell wall formation during growth. In: Biochemistry of plant cell walls. Soc. Exp. Biol. 28, 177–197

    Google Scholar 

  • Northcote, D.H. (1989) Control of plant cell wall biogenesis. In: Plant cell wall polymers, biogenesis and biodegradation, pp. 1–15, Lewis, N.G., Paice, M.G., eds. ACS Symposium series 399, ACS, Washington

    Google Scholar 

  • Pezet-Si Mohammed, Y. (1987) Caractérisation des potentialités morphogènes du châtaignier Castanea sativa Miller. Distribution et possibilités de translocation des réserves insolubles et solubles associées à des gradients de pH intracellulaires. Thèse, Université de Clermont-Ferrand, France

    Google Scholar 

  • Piantini, U., Sorenson, O.W., Ernst, R.R. (1982) Multiple quantum folters for elucidating NMR coupling networks. J. Am. Chem. Soc. 104, 6800–6801

    Google Scholar 

  • Rao, K.S. (1985) Seasonal ultrastructural changes in the cambium of Aesculus hippocastanum L. Ann. Sci. Bot. Paris, 13ème série, 7, 213–228

    Google Scholar 

  • Rayle, D.L., Cleland, R.E. (1977) Control of plant cell enlargement by hydrogen ions. Current Topics Dev. Biol. 11, 187–214

    Google Scholar 

  • Roland, J.C. (1978) Early differences between radial walls and tangential walls of actively growing cambial zone. IAWA Bull. 1, 7–10

    Google Scholar 

  • Savidge, R.A. (1990) Phytohormonal regulation of cambial growth in trees. In: Fast growing trees and nitrogen fixing trees, pp. 142–151, Werner, D., Müller, P., eds. Gustav Fischer Verlag, Stuttgart

    Google Scholar 

  • Simson, B.W., Timell, T.E. (1978a) Polysaccharides in cambial tissues of Populus tremuloides and Tilia americana. I — Isolation, fractionation, and chemical composition of the cambial tissues. Cellulose Chem. Technol. 12, 39–50

    Google Scholar 

  • Simson, B.W., Timell, T.E. (1978b) Polysaccharides in cambial tissues of Populus tremuloides and Tilia americana. II — Isolation, and structure of a xyloglucan. Cellulose Chem. Technol. 12, 51–62

    Google Scholar 

  • Simson, B.W., Timell, T.E. (1978c) Polysaccharides in cambial tissues of Populus tremuloides and Tilia americana. III — Isolation and constitution of an arabinogalactan. Cellulose Chem. Technol. 12, 63–77

    Google Scholar 

  • Simson, B.W., Timell, T.E. (1978d) Polysaccharides in cambial tissues of Populus tremuloides and Tilia americana. IV -4.0.methylglucuronoxylan and pectin. Cellulose Chem. Technol. 2, 79–84

    Google Scholar 

  • Slocum, R.D., Roux, S.J. (1982) An improved method for the subcellular localization of calcium using a modification of the antimonate precipitation technique. J. Histochem. Cytochem. 30, 617–629

    Google Scholar 

  • Suzuki, K., Ingoldd, E., Sugiyama, M., Komamine, A. (1991) Xylan synthase activity in isolated mesophyll cells of Zinnia elegans during differentiation to tracheary elements. Plant Cell Physiol. 32, 303–306

    Google Scholar 

  • Thiéry, J.P. (1967) Mise en évidence des polysaccharides sur coupes fines en microscopie électronique. J. Microscopic 6, 987–1018

    Google Scholar 

  • Wick, S.W., Hepler, P.K. (1980) Localization of Ca++-containing antimonate precipitates during mitosis. J. Cell Biol. 86, 500–513

    Google Scholar 

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Authors and Affiliations

  1. Laboratoire des Biomembranes et Surfaces Cellulaires Végétales URA 311, Ecole Normale Supérieure, 46 rue d'Ulm, F-75230, Paris Cedex 05, France

    Mathias Baïer, Renée Goldberg, Anne-Marie Catesson & Michèle Liberman

  2. Département de Chimie, URA 1679, Ecole Normale Supérieure, 24 rue Lhomond, F-75231, Paris Cedex 05, France

    Nadia Bouchemal, Véronique Michon & Catherine Hervé du Penhoat

Authors
  1. Mathias Baïer
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  2. Renée Goldberg
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  3. Anne-Marie Catesson
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  4. Michèle Liberman
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  5. Nadia Bouchemal
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  6. Véronique Michon
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  7. Catherine Hervé du Penhoat
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We acknowledge support from the French Ministry of Research and Technology and also from the European Program Eureka 447/ Eurosilva.

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Baïer, M., Goldberg, R., Catesson, AM. et al. Pectin changes in samples containing poplar cambium and inner bark in relation to the seasonal cycle. Planta 193, 446–454 (1994). https://doi.org/10.1007/BF00201825

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  • DOI: https://doi.org/10.1007/BF00201825

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