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The human transmembrane secretory component (poly-Ig receptor): molecular cloning, restriction fragment length polymorphism and chromosomal sublocalization

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Summary

The human transmembrane secretory component (SC) mediates glandular translocation of polymeric IgA and IgM into exocrine secretions. A 2898-bp cDNA clone, encoding the entire sequence of the human transmembrane SC, was isolated from a colonic adenocarcinoma cell line cDNA library. The deduced amino-acid sequence had a length of 764 residues and showed an overall similarity of 56% and 64% with the rabbit and rat counterpart, respectively. A restriction fragment length polymorphism (RFLP) was found with PvuII, revealing a two-alle RFLP with an autosomal codominant inheritance pattern and allele frequencies of 0.65 and 0.35. Southern blot analysis of human-rodent somatic hybrid panels, including hybrids with translocation chromosomes carrying different parts of chromosome 1, assigned the SC gene to 1q31-q42, thus confirming a previously reported provisional assignment.

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Authors and Affiliations

  1. Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology, University of Oslo The National Hospital, Rikshospitalet, N-0027, 1, Oslo, Norway

    P. Krajči & P. Brandtzaeg

  2. Institut für Humangenetik der Universität, Bahnhofstrasse 7a, W-3550, Marburg, Germany

    K. H. Grzeschik

  3. Department of Human Genetics, University Hospital Nijmegen, NL-6500, HB Nijmegen, The Netherlands

    A. H. M. Geurts van Kessel

  4. Institute for Forensic Medicine, University of Oslo, The National Hospital, Rikshospitalet, N-0027, 1, Oslo, Norway

    B. Olaisen

Authors
  1. P. Krajči
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  2. K. H. Grzeschik
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  3. A. H. M. Geurts van Kessel
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  4. B. Olaisen
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  5. P. Brandtzaeg
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Krajči, P., Grzeschik, K.H., van Kessel, A.H.M.G. et al. The human transmembrane secretory component (poly-Ig receptor): molecular cloning, restriction fragment length polymorphism and chromosomal sublocalization. Hum Genet 87, 642–648 (1991). https://doi.org/10.1007/BF00201717

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  • DOI: https://doi.org/10.1007/BF00201717

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