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Genetic polymorphism of desialyzed alpha2 HS-glycoprotein by ultrathin isoelectric focusing

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Summary

The genetically determined polymorphism of α2 HS-glycoprotein was analyzed by immunoblotting ultrathin-layer polyacrylamide gel isoelectric focusing in the pH range 4–6.5 and neuraminidase pretreated sera. In a Libyan population sample from Tripoli (n=110) three common phenotypes, α2 HSG 1–1, 2–1, and 2–2, were observed. The allele frequencies were α2 HSG1=0.8364 and α2 HSG2=0.1636. The theoretical exclusion rate in cases of disputed paternity is 11.8%.

Zusammenfassung

Der genetisch determinierte Polymorphismus des α2 HS-Glykoproteins wurde mit der isoelektrischen Fokussierung auf einer ultradünnen Schicht von Polyacrylamidgel in einem pH-Bereich von 4–6.5 und anschließenden Immunoblotting von Serumproben nach Behandlung mit Neuraminidase durchgeführt. Bei Libyern aus Tripoli (n=110) wurden die drei häufigen Phänotypen α2 HSG 1–1, 2–1 und 2–2 beobachtet. Folgende Allel-Frequenzen wurden ermittelt: α2 HSG1=0.8364 und α2 HSG2=0.1636. Aufgrund unserer Untersuchungsergebnisse errechnet sich für das α2 HSG-System eine theoretische Ausschlußchance von 11.8%.

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Authors and Affiliations

  1. Department of Forensic Medicine, Tohoku University School of Medicine, Sendai, Japan

    I. M. Sebetan

  2. Department of Forensic Medicine and Toxicology, Tanta University, Faculty of Medicine, Tanta, Egypt

    M. M. Heshmat

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  1. I. M. Sebetan
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  2. M. M. Heshmat
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Sebetan, I.M., Heshmat, M.M. Genetic polymorphism of desialyzed alpha2 HS-glycoprotein by ultrathin isoelectric focusing. Z Rechtsmed 101, 205–207 (1988). https://doi.org/10.1007/BF00201115

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  • DOI: https://doi.org/10.1007/BF00201115

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