Skip to main content
SpringerLink
Log in

Natural killer cell cytotoxic potential of patients with ovarian carcinoma and its modulation with virus-modified tumor cell extract

  • Original Articles
  • Published:
Cancer Immunology Immunotherapy Aims and scope Submit manuscript

Summary

We have demonstrated that cancer patients with ovarian carcinoma display deficient peripheral blood NK-cell cytotoxic potential against the K-562 target cell line. Furthermore, no NK-cell activity against the same tumor was detected in ascitic fluids of these patients. The inferior peripheral blood NK-cell cytotoxicity of ovarian carcinoma patients was significantly augmented after ID inoculation with virus-modified tumor cell extract. Similarly, NK-cell activity in the ascitic fluids was dramatically increased after IP in vivo therapy with the same tumor extract preparation. Interestingly, in some of the cancer patients the augmentation of NK-cell activity in ascitic fluids after IP injection of virus-modified tumor cells extract was associated with a clinical response of the patients, as demonstrated by regression of ascitic tumors. These studies indicate, first, that virus-modified tumor extract displays immunopotentiating activity, as reflected by its marked NK cell-augmenting potential, and secondly, that regional activation of NK cells could underlie the mechanism of regression of ascitic tumors.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Allavena P, Introna M, Mangloni C, Mantovani A (1981) Inhibition of natural killer activity by tumor-associated lymphoid cells from ascitic ovarian carcinomas. J Natl Cancer Inst 67:319

    PubMed  CAS  Google Scholar 

  2. Freedman RS, Bowen JM, Wharton JT, Rutledge FN, Hamberger AD (1980) Virus-modified homologous tumor-cell extract in the treatment of vulvar carcinoma. Cancer Immunol Immunother 8:33

    Article  Google Scholar 

  3. Hanna N, Fidler IJ (1980) Role of natural killer cells in the destruction of circulating tumor emboli. J Natl Cancer Inst 65:801

    PubMed  CAS  Google Scholar 

  4. Hanna N, Fidler IJ (1981) Relationship between metastatic potential and resistance to natural killer cell-mediated cytotoxicity in three murine tumor systems. J Natl Cancer Inst 66:1183

    Google Scholar 

  5. Herberman RB, Holden HT (1978) Natural cell-mediated immunity. Adv Cancer Res 27:305

    Article  PubMed  CAS  Google Scholar 

  6. Herberman RB, Ortaldo JR (1981) Natural killer cells: their role in defenses against disease. Science 214:24

    Article  PubMed  CAS  Google Scholar 

  7. Herberman RB, Ortaldo JR, Mantovani A, Hobbs DS, Kung H-F, Petska S (1982) Effect of human recombinant interferon on cytotoxic activity of natural killer (NK) cells and monocytes. Cell Immunol 67:160

    Article  PubMed  CAS  Google Scholar 

  8. Hersey P, Hobbs A, Edwards A, McCarthy WH, McGovern HJ (1982) Relationship between natural killer cell activity and histological features of lymphocyte infiltration and partial regression of the primary tumor in melanoma patients. Cancer Res 42:363

    PubMed  CAS  Google Scholar 

  9. Lotzová E (1983) Function of natural killer cells in various biological phenomena. Surv Synth Pathol Res 2:41

    Google Scholar 

  10. Lotzová E, McCredie KB (1978) Natural killer cells in mice and man and their possible biological significance. Cancer Immunol Immunother 4:215

    Article  Google Scholar 

  11. Lotzová E, Savary CA (1984) Stimulation of NK-cell cytotoxic potential of normal donors by two species of recombinant leucocyte interferon. Interferon Res 4 (in press)

  12. Lotzová E, Savary CA, Keating MJ (1982) Studies on the mechanism of defective natural killing in leukemia-diseased patients. Exp Hematol 10:83

    Google Scholar 

  13. Lotzová E, Savary CA, Stringfellow DA (1983a) 5-Halo-6-phenyl pyrimidinones: new molecules with cancer therapeutic potential and interferon-inducing capacity are strong inducers of murine natural killer cells. J Immunol 130:965

    PubMed  Google Scholar 

  14. Lotzová E, Savary CA, Quesada JR, Gutterman JU, Hersh EM (1983b) Analysis of natural killer cell cytotoxicity of cancer patients treated with recombinant interferon. J Natl Cancer Inst 71:903

    PubMed  Google Scholar 

  15. Lozzio CB, Lozzio BB (1972) Human chronic myelogenous leukemia cell line with positive Philadelphia chromosome. Blood 45:231

    Google Scholar 

  16. Mantovani A, Allavena P, Sessa C, Bolis G, Mangioni C (1980) Natural killer activity of lymphoid cells isolated from human ascitic ovarian tumors. Int J Cancer 25:573

    Article  PubMed  CAS  Google Scholar 

  17. Roder JC, Haliotis T, Klein M, Korec S, Jett J, Ortaldo J (1980) A new immunodeficiency disorder in human involving NK cells. Nature 284:533

    Article  Google Scholar 

  18. Sinkovics JG (1977) Immunotherapy with viral oncolysates for sarcoma. JAMA 237:869

    Article  PubMed  CAS  Google Scholar 

  19. Talmadge JE, Myers KM, Preius DJ, Starkey JR (1980) Role of NK cells in tumor growth and metastasis in beige mice. Nature 284:622

    Article  PubMed  CAS  Google Scholar 

  20. Tursz T, Dokhelar MC, Lipinski M, Amiel JL (1982) Low NK-cell activity and malignant lymphoma. In: Serrou B, Rosenfeld C, Herberman RB (eds) Natural killer cells. Elsevier Biomedical Press, Amsterdam, p 241

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Clinical Immunology and Biological Therapy, MD Anderson Hospital and Tumor Institute, Texas Medical Center, 77030, Houston, TX, USA

    Eva Lotzová & C. A. Savary

  2. Department of Gynecology, MD Anderson Hospital and Tumor Institute, Texas Medical Center, 77030, Houston, TX, USA

    R. S. Freedman

  3. Department of Tumor Biology, MD Anderson Hospital and Tumor Institute, Texas Medical Center, 77030, Houston, TX, USA

    J. M. Bowen

Authors
  1. Eva Lotzová
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. C. A. Savary
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. R. S. Freedman
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. J. M. Bowen
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lotzová, E., Savary, C.A., Freedman, R.S. et al. Natural killer cell cytotoxic potential of patients with ovarian carcinoma and its modulation with virus-modified tumor cell extract. Cancer Immunol Immunother 17, 124–129 (1984). https://doi.org/10.1007/BF00200048

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00200048

Keywords

Search

Navigation