Skip to main content
SpringerLink
Log in

Suppressor cells in the effector phase of autologous cytotoxic reactions in cancer patients

  • Original Articles
  • Published:
Cancer Immunology, Immunotherapy Aims and scope Submit manuscript

Summary

Cytotoxicity was induced in lymphocytes (CL) from 10 out of 15 patients by autologous mixed lymphocyte tumor cell culture and further cultivation with recombinant interleukin-2. In cells from 3 of the 10 patients, cytotoxicity was suppressed by more than 50% when autologous peripheral blood mononuclear cells (PBMC) from the patients with large tumors were added to the autologous killing system. The cells responsible for suppressing the cytotoxicity in the effector phase were adherent or nonadherent to plastic depending on the patient examined. The T cell fraction from 1 patient significantly suppressed the cytotoxic activity, and this suppression was seen only in the autologous system. On the other hand, plastic adherent cells but not T cells from PBMC of 2 subjects suppressed the cytotoxic activity of CL. The reason why the main cell population suppressing the CL activity differed among the patients is unclear. However, the findings that the suppression was mostly abrogated following resection of the tumor mass suggested that suppressor cells, either of macrophage lineage or T cells, are induced in patients with a large tumor mass. This speculation is supported by the finding that the PBMC from a patient with tumor recurrence regained the suppressive activity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  1. Bluestone JA, Lopez C (1979) Suppression of the immune response in tumor-bearing mice. II Characterization of adherent suppressor cells. J Natl Cancer Inst 63:1221

    Google Scholar 

  2. Braun DP, Harris JE (1981) Relationship of leukocyte number, immunoregulatory cell function, and phytohemagglutinin responsiveness in cancer patients. J Natl Cancer Inst 67:809

    Google Scholar 

  3. Fujii T, Igarashi T, Kishimoto S (1987) Significance of suppressor macrophages for immunosurveillance of tumor-bearing mice. J Natl Cancer Inst 78:509

    Google Scholar 

  4. Fujimoto S, Green MI, Sehon AH (1976) Regulation of the immune response to tumor antigens. II. The nature of immuno-suppressor cells in tumor-bearing hosts. J Immunol 116:800

    Google Scholar 

  5. Garrigues HJ, Romero P, Hellstrom I, Hellstrom KE (1981) Adherent cells (macrophages?) in tumor-bearing mice suppress MLC responses. Cell Immunol 60:109

    Google Scholar 

  6. Hinuma S, Naruo O, Shiho O, Tsukamoto K (1986) Characteristics of murin non-specific killer cells induced in vivo by recombinant human interleukin-2. Immunology 59:251

    Google Scholar 

  7. Hyde AM, Stag RB, McEachron R, Nutter RL (1985) Effects of the removal of adherent and phagocytic cells on the spleen cell lymphoproliferative response of tumor-bearing mice. Cancer Immunol Immunother 20:97

    Google Scholar 

  8. Ichino Y, Ishikawa T (1983) Cytolysis of autologous fresh osteosarcoma cells by human cytotoxic T lymphocytes propagated with T cell growth factor. Gann 74:584

    Google Scholar 

  9. Ichino Y, Ishikawa T (1984) Generation of human cytotoxic T lymphocytes against fresh autologous and allogeneic solid tumors by mixed lymphocyte tumor cell culture with T cell growth factor. Gann 75:436

    Google Scholar 

  10. Jerrells TR, Dean JH, Richardson GL, McCoy JL, Herberman RB (1978) Role of suppression cells in depression of in vitro lymphoproliferative responses of lung cancer and breast cancer patients. J Natl Cancer Inst 61:1001

    Google Scholar 

  11. Julius MH, Simpson E, Herzenberg LA (1973) A rapid method for the isolation of functional thymus-derived murine lymphocytes. Eur J Immunol 3:645

    Google Scholar 

  12. Kumagai K, Ito K, Hinuma S, Tada M (1979) Pretreatment of plastic Petri dishes with fetal calf serum; A simple method for macrophage isolation. J Immunol Methods 29:17

    Google Scholar 

  13. Miller ME (1969) Phagocytosis in he newborn infant; Humoral and cellular factors. J Pediatr 74:255

    Google Scholar 

  14. Mokyr MB, Braun DP, Dray S (1979) Augmentation of antitumor cytotoxicity in MOPC-315 tumor bearer spleen cells by depletion of glass-adherent cells prior to in vitro activation. Cancer Res 39:785

    Google Scholar 

  15. Mukherji B, Wilhelm SA, Guha A, Ergin MT (1986) Regulation of cellular immune response against autologous human melanoma. I. Evidence for cell-mediated suppression of in vitro cytotoxic immune response. II. Mechanism of induction and specificity of suppression. J Immunol 136:1888

    Google Scholar 

  16. North RJ, Bursuker I (1984) Generation and decay of the immune response to a progressive fibrosarcoma; I Lytl+2− effector T cells. J Exp Med 159:1295

    Google Scholar 

  17. Oldman RK, Dean JH, Cannon GB, Oratldo JR, Dunston G, Applebaum F, McCoy JL, Djeu J, Herberman RB (1976) Cryopreservation of human lymphocyte function as measured by in vitro assay. Int J cancer 18:145

    Google Scholar 

  18. Robinson MK, Truitt GA, Okayasu T, Wheelock EF (1983) Enhanced suppressor macrophage activity associated with termination of the L5178Y cell tumor-dormant state in DBA/2 mice. Cancer Res 43:5831

    Google Scholar 

  19. Takei F, Levy JG, Kilburn DG (1977) Characterization of suppressor cells in mice bearing syngeneic mastocytoma. J Immunol 118:412

    Google Scholar 

  20. Ting CC, Hargrove ME (1982) Tumor cell-triggered macrophage-mediated suppression of the T-cell cytotoxic response to tumor-associated antigens. II. Mechanisms for induction of suppression — J Natl Cancer Inst 69:873

    Google Scholar 

  21. Ting CC, Rodorigues D (1982) Tumor cell-triggered macrophage-mediated suppression of the T-cell cytotoxic response to tumor-associated antigens. I. Characterization of the cell components for induction of suppression. J Natl Cancer Inst 69:867

    Google Scholar 

  22. Vanky F, Gorsky T, Masucci MG, Klein E (1982) Lysis of tumor biopsy cells by autologous T lymphocytes activated in mixed cultures and propagated with T cell growth factor. J Exp Med 155:83

    Google Scholar 

  23. Varesio L, Giovarelli M, Landolfo S, Forni G (1979) Suppression of proliferative response and lymphokine production during the progression of a spontaneous tumor. Cancer Res 39:4983

    Google Scholar 

  24. Vose BM, Bonnard GD (1982) Specific cytotoxicity against autologous tumor and proliferative responses of human lymphocytes grown in interleukin 2. Int J Cancer 29:33

    Google Scholar 

  25. Whitney RB, Levy JG, Smith AG (1974) Influence of tumor size and surgical resection on cell-mediated immunity in mice. J Natl Cancer Inst 53:111

    Google Scholar 

  26. Ye QW, Kokyr MB, Pyle JM, Dray S (1984) Suppression of antitumor immunity by macrophages in spleens of mice bearing a large MOPC-315 tumor. Cancer Immunol Immunother 16:162

    Google Scholar 

  27. Young Mr, Wheeler E, Newby M (1986) Macrophage-mediated suppression of natural killer cell activity in mice bearing Lewis lung carcinoma. J Natl Cancer Inst 76:745

    Google Scholar 

  28. Yu A, Watts H, Jaffe N, Robertson P (1977) Concomitant presence of tumor-specific cytotoxic and inhibitor lymphocytes in patients with osteogenic sarcoma. N Engl J Med 297:121

    Google Scholar 

  29. Zembala M, Mytar B, Popiela T, Asherson GL (1977) Depressed in vitro peripheral blood lymphocyte response to mitogens in cancer patient; The role of suppressor cells. Int J Cancer 19:605

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Otolaryngology, School of Medicine, Kumamoto University, 1-1-1 Honjo, 860, Kumamoto, Japan

    Masao Eura, Tatsuhiko Maehara, Tsutomu Ikawa & Takeru Ishikawa

Authors
  1. Masao Eura
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Tatsuhiko Maehara
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Tsutomu Ikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Takeru Ishikawa
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Eura, M., Maehara, T., Ikawa, T. et al. Suppressor cells in the effector phase of autologous cytotoxic reactions in cancer patients. Cancer Immunol Immunother 27, 147–153 (1988). https://doi.org/10.1007/BF00200020

Download citation

  • Received:

  • Accepted:

  • Issue Date:

  • DOI: https://doi.org/10.1007/BF00200020

Keywords

Search

Navigation