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A p50 surface antigen restricted to human urinary bladder carcinomas and B lymphocytes

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Summary

We have previously described the derivation of a monoclonal antibody, S2C6, to a novel 50 Kdalton antigen associated with human urinary bladder carcinoma. No reactions were obtained with carcinomas of unrelated origin or with normal urothelial cells. However, the antibody also reacted with a similar antigen on some cell lines of B lymphocyte origin. Using large panels of target cells we have now shown that this reactivity was entirely restricted to cells of the B lineage within the haematopoietic system. As opposed to its apparent restriction to malignant cells of the urothelium, the S2C6 antigen was expressed by normal B lymphocytes as well as by many malignant B cells (chronic lymphocytic leukaemia, hairy cell leukaemia and immunocytoma). Pre-B cells derived from acute lymphocytic leukaemia and plasma cells from multiple myeloma lacked the antigen. Expression was significantly enhanced on cultured B cells from Burkitt lymphomas and on Epstein-Barr virus-transformed lymphoblastoid cell lines including those of the pre-B phenotype derived from fetal bone marrow. As judged from the molecular size and the distribution pattern displayed by the S2C6 antigen it appears to be distinct from other B cell antigens previously described. A possible relation of the S2C6 antigen to a receptor for B cell growth factors is discussed.

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Authors and Affiliations

  1. Department of Immunology, University of Stockholm, S-106 91, Stockholm, Sweden

    Staffan Paulie, Hannu Koho, Hedi Ben-Aissa & Peter Perlmann

  2. Department of Tumor Biology, Karolinska Institute, S-10401, Stockholm, Sweden

    Barbro Ehlin-Henriksson

  3. Radiumhemmet, Karolinska Hospital, S-10401, Stockholm, Sweden

    Håkan Mellstedt

Authors
  1. Staffan Paulie
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  2. Barbro Ehlin-Henriksson
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  3. Håkan Mellstedt
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  4. Hannu Koho
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  5. Hedi Ben-Aissa
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  6. Peter Perlmann
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Paulie, S., Ehlin-Henriksson, B., Mellstedt, H. et al. A p50 surface antigen restricted to human urinary bladder carcinomas and B lymphocytes. Cancer Immunol Immunother 20, 23–28 (1985). https://doi.org/10.1007/BF00199769

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  • DOI: https://doi.org/10.1007/BF00199769

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