Summary
Microbial infection causes inflammation which stimulates macrophage functions. One of the inflammatory products, lysophosphatidylcholine (lyso-Pc), can stimulate macrophage activities. Treatment of mice with lyso-Pc enhanced spreading and ingestion activities of peritoneal macrophages. In vitro treatment of macrophages with lyso-Pc greatly enhanced spreading but not ingestion activities. However, incubation of a mixture of adherent and nonadherent cells with lyso-Pc produced a markedly enhanced ingestion activity of macrophages, implying the contribution of nonadherent cells to the stimulation of macrophages. Time course studies of the stimulation of these macrophages showed that spreading activity is stimulated immediately, even 30 min, after their contact with lyso-Pc while induction of ingestion activity requires a latent period of about 5 h. When the specificity of the macrophage receptors for ingestion was analyzed using defined immunoglobulins (i.e., IgG and IgM) with or without complement, lyso-Pc-activated macrophages efficiently ingested IgG-coated sheep erythrocytes independent of complement. However, macrophages of the same lyso-Pc-treated mice did not ingest erythrocytes coated with IgM and complement. These observations suggest that lyso-Pc-stimulated macrophages ingest the targets via Fc-receptors but not C3b receptors.
Similar content being viewed by others
References
Andreesen R, Modolell M, Weltizien HU, Eibl H, Common HH, Lohr GW, Munder PG (1978) Selective destruction of human leukemic cells by alkyl lysophospholipids. Cancer Res 38:3894
Berdel WE, Fink U, Egger B, Reichert A, Munder PG, Rastetter J (1981) Inhibition by alkyl-lysophospholipids of tritiated thymidine uptake in cells of human malignant urologic tumors. J Natl Cancer Inst 66:813
Berdel WE, Greiner E, Fink M, Stavrou D, Reichert A, Rastetter J, Snyder F (1982) Cytotoxic effect of alkyl-lysophospholipids (ALP) in rat brain tumor cells. Proc Am Soc Cancer Res p 198 No. 782
Berdel WE, Fink U, Rastetter J (1982) Phase I study of alkyl-lysophospholipids (ALP) in advanced malignant disease. Proc Am Soc Clin Oncol p 18, No C71
Bianco C, Griffin FM, Silverstein SC (1975) Studies of macrophage complement receptors. Alteration of receptor function upon macrophage activation. J Exp Med 141:1278
Bianco C, Eden AA, Cohn AZ (1976) The induction of macrophage spreading: role of coagulation factors and the complement system. J Exp Med 144:1531
Chien KR, Abrams J, Serioni A, Martin JT, Farber JL (1978) Accelerated phospholipid degradation and associated membrane dysfunction in irreversible ischemic liver cell inury. J Biol Chem 253:4809
Chon ZA, Benson B (1975) The differentiation of mononuclear phagocytes, morphology, cytochemistry, and biochemistry. J Exp med 121:153
Davidson I, Nelson DA (1969) The blood. In: Davidson I, Henry JB (eds) Todd-Sanford Clinical diagnosis by laboratory methods 14th edn. W. B. Sauners Company, Philadelphia, p 120
Doe WF, Henson PM (1978) Macrophage stimulation by bacterial lipopolysaccharides. I. Cytolytic effect on tumor target cells. J Exp Med 148:544
Elin RJ, Wolff SM, Cheolid L (1976) Non-specific resistance to infection induced in mice by a water soluble adjuvant derived from Mycobacterium smegmatis J Infect Dis 133:500
Gillett MPT, Besterman EMM (1975) Plasma concentration of lysolecithin and other phospholipids in the healthy population and in men suffering from atherosclerosis diseases. Atherosclerosis 22:111
Goerke J, deGier J, Bensen PPM (1971) Silica gel stimulates the hydrolysis of lecithin by phospholipase A. Biochim Biophys Acta 248:245
Griffin FM, Silverstein SC (1974) Segmental response of the macrophages plasma membrane to a phagocytic stimulus. J Exp Med 139:323
Hoerni B, Durand M, Richard P, deMascarel A, Hoerni-Simon G, Chauvegne J, Lagarde C (1979) Successful maintenance immunotherapy by BCG of non-Hidgkin's Malignant lymphomas: Results of controlled trail. Br J Haematol 42:507
Hoerni B, Durand M, Eghbali H, Hoerni-simon G, Richard P, deMascarel A (1982) Maintenance immunotherapy with BCG in non-Hodgkin's malignant lymphomas: A progress report of a randomized trial. In: Mathe G, Bonadonna G, Salmon S (eds) Adjuvant therapies of cancer. Springer-Verlag, Berlin, Heidelberg, New York, p 92
Le Mevel BP (1982) Ewing's Sarcoma: 5 year survival under adjuvant chemotherapy. In: Mathe G, Bonadonna G, Salmon S (eds) Adjuvant therapies of cancer. Springer-Verlag, Berlin, Heidelberg, New York, p 128
Lowe J, Shore DF, Iles PB, Langman MJS, Baldwin RW (1980) Intrapleural BCG in operable lung cancer. Lancet I:11
Mantovani B, Robinovitch M, Nussenszweig V (1972) Phagocytosis of murine complexes by macrophages. Different roles of the macrophage receptor sites for complement (C3) and for immunoglobulin (IgG) J Exp Med 135:780
McKneally MF, Maver C, Kausel HW (1976) Regional immunotherapy of lung cancer with intrapleural BCG. Lancet I:377
Modell M, Munder PG (1972) The action of purified phospholipase B in inflammation and immunity. Int Arch Allergy 43:724
Munder PG, Modelell M, Ferber E, Fischer H (1970) The relationship between macrophages and adjuvants activity. In: Furth R (ed) Mononuclear phagocytes. Blackwell Scientific Publications, Oxford, p 445
Munder PG, Fischer H, Weltzien HU, Oettgen HW, Westphal O (1976) Lysolecithin analogs: a new class of immunopotentiators with antitumor activity. Proc Am Assoc Cancer Res 17:174
Munder PG, Modelell M, Andreesen R, Weltzien HU, Westphal O (1980) Lysophosphatidylcholine (Lysolecithin) and its synthetic analogs, immunomodulating and other biological effects. In: Chedid L, Miescher PA, Mueller-Eberhard HJ (eds) Immunomodulating: Springer Semin Immunopath Vol 2 No 1–2. Springer-Verlag, Berlin P 177
Neumann C, Sorg C (1980) Sequential expression of functions during macrophage differentiation in murine bone marrow liquid cultures. Eur J Immunol 10:834
Newman SL, Musson RA, Henson PM (1980) Development of functional complement receptors during in vitro maturation of human monocytes into macrophages. J Immunol 125:2236
Ngwenya BZ, Yamamoto N (1985) Activation of peritoneal macrophages by lysophosphatidylcholine. Biochim Biophys Acta 839:9
Ottolenghi A, Kocan AA, Weatherly NF, Larsh JE (1975) Nipposdtrongylus brasiliensis: Phospholipase in nonsensitized and sensitized rats after challenge. Exp Parasitol 38:96
Rapp HJ (1976) Immunotherapy of experimental cancer with BCG. In: Crowell RL, Friedman H, Prier JE (eds) Tumor virus infections and immunity. University Park Press, Baltimore p 261
Runge MH, Andreesen R, Pfleideres A, Munder PG (1980) Destruction of human solid tumors by alkyl-lysophospholipids. J Natl Cancer Inst 64:1301
Saluk PH, Edmundowicz S, Wrighley DM, Tabor DR (1981) Co-expression of enhanced spreading, ingestion, and inhibition of tumor cell proliferation by macrophages from lipopolysaccharide injected mice. Cancer Immunol Immunother 11:159
Senn HJ, Amgwerd R, Jungi WF, Hochuli R, Ammann J, Engelhart G, Heinz C, Wick A, Enderlin F, Greux G, Simon B, Lanz R, Bigler R, Seiler S (1982) Adjuvant chemoimmunotherapy with LMF plus BCG in node-negative and node-positive breast cancer-intermediate report at 4 years. In: Mathe G, Bonadonna G, Salmon S (eds) Adjuvant therapies of cancer. Springer-Verlag, Berlin, Heidelberg, New York p 177
Snyder F, Maline B, Piantodosi C (1973) Tetrahydropteridine-dependent cleavage enzyme for O-alkyl-lipids: substrate specificity. Biochim Biophys Acta 316:259
Soodsma JF, Piantodosi C, Snyder F (1970) The biocleavage of aklyl-glyceryl ethers in Morris hepatomas and other transplantable neoplasms. Cancer Res 30:309
Tabor DR, Saluk PH (1981) The functional heterogenecity of murine-resident macrophages to a chemotactic signal and induction of C3b receptor-mediated ingestion. Immunol Lett 3:371
White RG, Coons HH, Connally JM (1955) Studies on antibody production III. The alum granuloma. J Exp Med 102:73
Wrigley DM, Saluk PH (1981) Induction of C3b-mediated phagocytosis in macrophages by distinct populations of lipopolysaccharide-stimulated lymphocytes. Infect, Immun 34:780
Yamamoto N, Kawada K, Ochiai H, Droffner ML, Ngwenya BZ (1983) Activation of macrophages by lysophosphatidylcholine and their analogs. Abst Am Soc Microbiol, p 25
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Ngwenya, B.Z., Yamamoto, N. Effects of inflammation products on immune systems. Cancer Immunol Immunother 21, 174–182 (1986). https://doi.org/10.1007/BF00199358
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1007/BF00199358