Abstract
Photon fluence rate-response curves at different wavelengths were generated for the light-induced inhibition of hypocotyl elongation in seedlings of wildtype and photomorphogenic mutants of Arabidopsis thaliana. (L.) Heynh. Treatment of wild-type seedlings with continuous low-fluence-rate light (< 1.0 μmol photons · m−2 · s−1) induced some inhibition of hypocotyl elongation at all wavelengths tested, with maximum inhibition in blue light. At higher fluence rates, inhibition reached a maximum of 70–80% in UV-A, blue, and far-red light. Fluence rate-response curves for seedlings of blu1, a blue light-response mutant, showed a specific reduction in their response to blue light, but their response to UV-A, red, and far-red light was similar to that in wild-type seedlings. In contrast, the phytochromedeficient mutant hy6 showed a loss of response to lowfluence-rate light at all wavelengths, as well as to highfluence-rate far-red light. However, hy6 seedlings retained sensitivity to high-fluence-rate blue and UV-A light. The data support the conclusion that blue-lightand phytochrome-dependent photosensory systems regulate hypocotyl elongation independently and in an additive manner. Furthermore, hypocotyl inhibition in wild-type, blul, hy6 and blul-hy6 double mutants was indistinguishable in UV-A light, whereas marked differences were observed at other wavelengths, indicating the involvement of a third photosensory system with an absorption maximum in the UV-A.
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We thank N.K. Peters and W.D. Bauer for critical reading of the manuscript and K.L. Poff for providing SAN 9789, seeds of am45, and several interference filters. This work was supported by National Science Foundation Grant No. DCB9106697 to R.P.H.
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Young, J.C., Liscum, E. & Hangarter, R.P. Spectral-dependence of light-inhibited hypocotyl elongation in photomorphogenic mutants of Arabidopsis: evidence for a UV-A photosensor. Planta 188, 106–114 (1992). https://doi.org/10.1007/BF00198946
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DOI: https://doi.org/10.1007/BF00198946