Abstract
Hepatitis C virus (HCV)-RNA in sera of patients with viral hepatitis C is supposed to be included, at least partially, into HCV particles. We found that the density of HCV-RNA-carrying material was variable, as determined by sucrose gradient density centrifugation (1.03–1.20 g/cm3). In some of the sera examined HCV-RNA was restricted to low densities between 1.03 and 1.08 g/cm3. In other sera additional densities of HCV-RNA were found distributed over the whole gradient with peaks at 1.12 and 1.17 and at 1.19–1.20 g/cm3. HCV-RNA banding at low densities could be completely co-precipitated with anti-β lipoprotein, whereas HCV-RNA fractions of higher densities were only partially precipitated or not at all. In 8 of 20 sera directly examined, HCV-RNA could be completely and in 9 sera only partially co-precipitated by anti-β lipoprotein. In 3 sera no significant precipitation could be observed.
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References
Beach M, Bradley D (1991) Analysis of the putative nonstructural region of hepatitis C virus. In: Hollinger FB, Lemon S, Magolis HS (eds) Viral hepatitis and liver disease. Williams and Wilkins, Baltimore, 376–381
Bradley DW, Maynard JD, Popper H, Cook EH, Ebert JW, McCaustland KA, Schable CA, Fields HS (1983) Posttransfusion non-A, non-B hepatitis: Physicochemical properties of two distinct agents. J Infect Dis 148:254–265
Bradley DW (1985) The agents of non-A, non-B viral hepatitis. J Virol Methods 10:307–319
Bradley DW, Krawczynski K, Beach MJ, Purdy MA (1991 a) Non-A, Non-B hepatitis: toward the discovery of hepatitis C and E viruses. Semin Liver Dis 11:128–146
Bradley DW, McCaustland K, Krawczynski K, Speibring J, Humphrey C, Cook EH (1991b) Hepatitis C virus: Buoyant density of the factor VIII-derived isolate in sucrose. J Med Virol 34:206–208
Choo Q-L, Weiner AJ, Overby LR, Kuo G, Houghton M, Bradley DW (1990) Hepatitis C virus: the major causative agent of viral non-A, non-B hepatitis. Br Med Bull 46:423–441
Feinstone SM, Mihalik K, Kamimura T, Alter HJ, London WT, Purcell RH (1983) Inactivation of hepatitis B virus and non-A, non-B hepatitis by chloroform. Infect Immun 41:816–821
Garson JA, Tuke PW, Maris M, Briggs M, Machin SJ, Preston FE, Tedder RS (1990) Demonstration of viremia patterns in haemophiliacs treated with hepatitis C-virus-contaminated factor VIII concentrates. Lancet II:1022–1025
Gribskov M, Burges RR (1986) Sigma factors from E. coli, B. subtilis, phage SP01, and phage T4 are homologous proteins. Nucleic Acids Res 14:6745–6763
Han JH, Shyamala V, Richman KH, Brauer MJ, Irvine B, Urdea MS, Tekamp-Olsen P, Kuo G, Choo Q-L, Houghton M (1991) Characterization of the terminal regions of hepatitis C viral RNA: identification of conserved sequences in the 5′ untranslated region and poly(A) tails at the 3′ end. Proc atl Acad Sci USA 88:1711–1715
He LF, Alling D, Popkin T, Shapiro M, Alter HJ, Purcell RH (1987) Determining the size of non-A, non-B hepatitis virus by filtration. J Infect Dis 156:636–640
Kodama T, Reddy P, Kishimoto C, Krieger M (1988) Purification and characterization of a bovine acetyl low density lipoprotein receptor. Proc Natl Acad Sci USA 85:9238–9242
Laufs R, Thomssen R (1968) Inhibition der Rubellavirus-Hämagglutination. Eigenschaften eines in fetalem Kälberserum vorkommenden Inhibitors der Rubellavirus-Hämagglutination in vitro. Arch Gesamte Virusforsch 24:181–191
Miller RH, Purcell H (1990) Hepatitis C virus shares amino acid similarity with pestiviruses and flaviviruses as well as members of two plant virus supergroups. Proc Natl Acad Sci USA 87:2057–2061
Reed LJ, Muench H (1938) A simple method of estimating fifty percent endpoints. Am J Hyg 27:493–497
Takamizawa A, Mori C, Fuke I, Manabe S, Murakami S, Fujita J, Onishi E, Andoh T, Yoshida I, Okayama H (1991) Structure and organization of the hepatitis C virus genome isolated from human carriers. J Virol 65:1105–1113
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Thomssen, R., Bonk, S., Propfe, C. et al. Association of hepatitis C virus in human sera with β-lipoprotein. Med Microbiol Immunol 181, 293–300 (1992). https://doi.org/10.1007/BF00198849
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DOI: https://doi.org/10.1007/BF00198849