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The chlorophyll-a/b proteins of photosystem II in Chlamydomonas reinhardtii

Isolation, characterization and immunological cross-reactivity to higher-plant polypeptides

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Abstract

We have adapted the procedure for the isolation of PSII membranes from higher plants (D.A. Berthold et al., 1981, FEBS Lett. 134, 231–234) to the green algae Chlamydomonas reinhardtii. The chlorophyll (Chl)-binding proteins from this PSII preparation have been further separated into single Chl-binding polypeptides and characterized spectroscopically. Seven single polypeptides were shown to bind Chl a and Chl b. In particular, we demonstrate that polypeptides p9, p10 and p22, which had not been previously shown to bind Chl a and b, have characteristics similar to those of CP29, CP26 and CP24 from higher plants. We note, however, that p9 and p10 are phosphorylatable in C. reinhardtii, at variance with CP29 and CP26 from higher plants. Our data support the notion that the PSII antenna systems in C. reinhardtii and in higher plants are very similar. Therefore, studies on the organization and regulation of light-harvesting processes in C. reinhardtii may provide information of general relevance for both green algae and higher plants.

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Abbreviations

Chl:

chlorophyll

IEF:

isoelectrofocusing

LHC:

light harvesting complex

MW:

molecular weight

PAGE:

polyacrylamide gel electrophoresis

PS:

photosystem

RC:

reaction centre

SDS:

sodium dodecylsulfate

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Authors and Affiliations

  1. Dipartimento di Biologia, Università di Padova, via Trieste 75, I-35100, Padova, Italy

    Roberto Bassi

  2. Service de Photosynthèse, Institut de Biologie Physico-Chimique, 13 rue P. et M. Curie, F-75005, Paris, France

    Francis-André Wollman

Authors
  1. Roberto Bassi
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  2. Francis-André Wollman
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Additional information

We thank Dr. J. Olive (Institut Jacques Monod, Paris, France) for the electron-microscopy analysis, C. de Vitry (Institut de Biologie Physico-Chimique, Paris, France) for the kind gift of a PSII RC preparation and P. Dainese and M.L. Di Paolo (Universitá di Padova, Padova, Italy) for helpfull discussions. Professor Strasser and Elizbeth Scwartz (Université de Genova, Genova, Switzerland) are thanked for assistance in taking low-temperature fluorescence emission spectra. Roberto Bassi was recipient of a short-term fellowship from the European Molecular Biology Organization fellowship, during the early phases of the work.

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Bassi, R., Wollman, FA. The chlorophyll-a/b proteins of photosystem II in Chlamydomonas reinhardtii . Planta 183, 423–433 (1991). https://doi.org/10.1007/BF00197742

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  • DOI: https://doi.org/10.1007/BF00197742

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