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Cytokinin controls the cell cycle at mitosis by stimulating the tyrosine dephosphorylation and activation of p34cdc2-like H1 histone kinase

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Abstract

In excised pith parenchyma from Nicotiana tabacum L. cv. Wisconsin Havana 38, auxin (naphthalene-1-acetic acid) together with cytokinin (6-benzylaminopurine) induced a greater than 40-fold increase in a p34cdc2-like protein, recoverable in the p13suc1-binding fraction, that had high H1 histone kinase activity, but enzyme induced without cytokinin was inactive. In suspension-cultured N. plumbaginifolia Viv., cytokinin (kinetin) was stringently required only in late G2 phase of the cell division cycle (cdc) and cells lacking kinetin arrested in G2 phase with inactive p34cdc2-like H1 histone kinase. Control of the Cdc2 kinase by inhibitory tyrosine phosphorylation was indicated by high phosphotyrosine in the inactive enzyme of arrested pith and suspension cells. Yeast cdc25 phosphatase, which is specific for removal of phosphate from tyrosine at the active site of p34cdc2 enzyme, was expressed in bacteria and caused extensive in-vitro activation of p13suc1-purified enzyme from pith and suspension cells cultured without cytokinin. Cytokinin stimulated the removal of phosphate, activation of the enzyme and rapid synchronous entry into mitosis. Therefore, plants can control cell division by tyrosine phosphorylation of Cdc2 but differ from somatic animal cells in coupling this mitotic control to hormonal signals.

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Abbreviations

BAP:

6-benzylaminopurine

BrdUrd:

5-bromo-2-deoxyuridine

cdc:

cell division cycle

Cdc25:

cdc phospho-protein phosphatase

CKI:

cyclin dependent kinase inhibitor

2,4-D:

2,4-dichlorophenoxyacetic acid

DAPI:

4′,6′ diamidino-2-phenylindole

GST-cdc25:

glutathione sulfur transferase-truncated cdc25 fusion

MS:

Murashige and Skoog (1962)

NAA:

naphthalene-1-acetic acid

p34cdc2 :

34-kDa product of the cdc2 gene

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Authors and Affiliations

  1. Plant Cell Biology Group, Research School of Biological Sciences, The Australian National University, 2601, ACT, Australia

    Kerong Zhang, David S. Letham & Peter C. L. John

  2. Cooperative Research Centre for Plant Science, GPO Box 475, 2601, Canberra, ACT, Australia

    David S. Letham & Peter C. L. John

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  1. Kerong Zhang
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  2. David S. Letham
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  3. Peter C. L. John
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Zhang, K., Letham, D.S. & John, P.C.L. Cytokinin controls the cell cycle at mitosis by stimulating the tyrosine dephosphorylation and activation of p34cdc2-like H1 histone kinase. Planta 200, 2–12 (1996). https://doi.org/10.1007/BF00196642

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  • DOI: https://doi.org/10.1007/BF00196642

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