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Fas (CD95) expression on CD4+ T cells from HIV-infected patients increases with disease progression

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Abstract

Active T cell suicide (apoptosis) is supposed to be involved in the CD4+ T cell depletion in the course of HIV infection. We investigated the expression of the apoptosis-related antigen Fas on CD4+ T cells from 25 HIV-positive individuals (CDC I-III) and 8 HIV-negative controls by two-colour flowcytometry. In addition, we evaluated: total CD4 count, HIV p24 antigen concentration in serum after immune complex dissociation, and clinical course of infection in HIV-positive individuals. We found a significant increase in mean Fas expression on CD4+ T cells from HIV-positive individuals compared to HIV-negative individuals (85.84±14.92% vs. 64.28±7.59%, P<0.001). Within the HIV-positive group the increase in Fas expression was correlated with the decline in CD4 count (r=−0.76, P<0.001), p24 antigen concentration in serum after immune complex dissociation (r=0.67, P<0.001), and CDC stage (r=0.73, P<0.001). The upregulation of Fas antigen on CD4 cells is associated with CD4 depletion and other virological and clinical marker of disease progression in HIV infection.

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Abbreviations

AIDS :

Acquired immunodeficiency syndrome

CDC :

Center of disease control

CTL :

Cytotoxic T lymphocyte

FasL :

Fas ligand

HIV :

Human immunodeficiency virus

MFI :

Mean fluorescence intensity

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Authors and Affiliations

  1. Department of Internal Medicine, Medical University of Lübeck, Ratzeburger Allee 160, D-23538, Lübeck, Germany

    S. P. Aries, B. Schaaf, C. Müller & K. Dalhoff

  2. Institute for Medical Microbiology, Medical University of Lübeck, Ratzeburger Allee 160, D-23538, Lübeck, Germany

    R. H. Dennin

Authors
  1. S. P. Aries
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  2. B. Schaaf
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  3. C. Müller
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  4. K. Dalhoff
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  5. R. H. Dennin
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Aries, S.P., Schaaf, B., Müller, C. et al. Fas (CD95) expression on CD4+ T cells from HIV-infected patients increases with disease progression. J Mol Med 73, 591–593 (1995). https://doi.org/10.1007/BF00196352

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  • DOI: https://doi.org/10.1007/BF00196352

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