Summary
Human apolipoprotein A-IV (apo A-IV) exhibits a genetic polymorphism with two common alleles, A-IV1 and A-IV2, in Caucasian populations. We have investigated this polymorphism in the Icelandic population. The frequencies of the two alleles are significantly different from middel European populations with a higher frequency of the A-IV2 allele (0.117 versus 0.077) occurring in Iceland. The alleles at the apo A-IV locus have significant effects on plasma high density lipoprotein cholesterol (HDL-C) and triglyceride levels. The average effect of the A-IV2 allele is to raise HDL-C by 4.9 mg/dl and to lower triglyceride levels by 19.4mg/dl. We estimate that the genetic variability at the apo A-IV gene locus accounts for 3.1% of the total variability of HDL-C and for 2.8% of the total variability of triglycerides in the population from Iceland. This confirms and extends our previous observations on apo A-IV allele effects in Tyroleans in an independent population.
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References
Becker PE (1975) Humangenetik, vol I/3. Thieme, Stuttgart
Beisiegel U, Utermann G (1979) An apolipoprotein homology of rat apolipoprotein A-IV in human plasma: isolation and partial characterization. Eur J Biochem 93:601–608
Boerwinkle E, Sing CF (1986) Roles of the apolipoprotein E and A-IV polymorphism in determining plasma lipid and apolipoprotein levels (abstract). 7th International Congress of Human Genetics, Berlin 1986, part II, pp 751–752
Boerwinkle E, Utermann G (1988) Simultaneous effects of the apolipoprotein E polymorphism on apolipoprotein E, apolipoprotein B, and cholesterol metabolism. Am J Hum Genet 42:104–112
Dvorin E, Gordon NL, Benson DM, Gotto AM Jr (1986) Apolipoprotein A-IV. A determinant for binding and uptake of high density lipoproteins by rat hepatocytes. J Biol Chem 261:15714–15718
Euro Report and Studies 98 (1984) Primary prevention of coronary heart disease. Report on a WHO meeting. WHO, Geneva
Green PHR, Glickman RM, Sandek CD, Blum CB, Tall AR (1979) Human intestinal lipoproteins. Studies in chyluric subjects. J Clin Invest 64:233–242
Karathanasis SK (1985) Apolipoprotein multigene family: tandem organisation of apolipoprotein A-IV, A-I and C-III genes. Proc Natl Acad Sci USA 82:6374–6378
Menzel HJ, Kladetzky RG, Assmann G (1982a) One-step screening method for the polymorphism of apolipoprotein A–I, A–II and A–IV. J Lipid Res 23:915–922
Menzel HJ, Kövary PM, Assmann G (1982b) Apolipoprotein A-IV polymorphism in man. Hum Genet 62:349–352
Menzel HJ, Boerwinkel E, Schrangel-Will S, Utermann G (1988) Human apolipoprotein A-IV polymorphism: frequency and effect on lipid and lipoprotein levels. Hum Genet 79:368–372
Neter J, Wasserman W (1974) Applied linear statistical model. Irwin, Homewood, Ill
Rosseneu M, Michiels G, De Keerspieter W, Bury I, De Slypese IP, Dieplinger H, Utermann G (1988) Human apolipoprotein A-IV quantitation by sandwich enzyme linked immunosorbent assay. Clin Chem 34:739–743
Sigurdsson G (1987) Lipids and diet in relation to cardiovascular disease in Iceland. Br J Clin Pract 41 [Suppl 54]: 54
Snedecor GW, Cochran WG (1980) Statistical methods, 7th edn. Iowa State University Press, Ames, Iowa
Stein O, Stein Y, Lefeure M, Roheim PS (1986) The role of apolipoprotein A-IV in reverse cholesterol transport studied with cultured cells and liposomes derived from an ether analog of phosphatidylcholine. Biochim Biophys Acta 878:7–13
Steinmetz A, Utermann G (1984) Activation of lecithin: choleserol-acyltransferase by human apolipoprotein A-IV. J Biol Chem 260:2258–2264
Utermann G, Feussner G, Franceschini G, Haas J, Steinmetz A (1982) Genetic variants of the group A apolipoproteins. Rapid methods for screening and characterization without ultracentrifugation. J Biol Chem 257:501–507
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Menzel, HJ., Sigurdsson, G., Boerwinkle, E. et al. Frequency and effect of human apolipoprotein A-IV polymorphism on lipid and lipoprotein levels in an Icelandic population. Hum Genet 84, 344–346 (1990). https://doi.org/10.1007/BF00196231
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DOI: https://doi.org/10.1007/BF00196231