Abstract
The uptake of [1-14C]isopentenyl diphosphate by intact plastids purified from cell suspensions of Vitis vinifera L. cv. Muscat de Frontignan was investigated using vacuum-filtration and silicone-oil-filtering techniques. Transport across the plastid envelope which was stimulated by cations, such as Mg2+ and Mn2+, was characterized by a K m of approx. 0.5 mM and a V max of 25 nmol·(mg protein)-1·-h-1. The data showed that isopentenyl diphosphate apparently accumulated in the plastid against a concentration gradient. The involvement of a protein carrier was suggested by the strong inhibition of the uptake by compounds which are known to block SH groups. Thus, the saturation kinetics together with the pH optimum (7.5–8), the temperature dependence (maximum incorporation at 37 °C) and the competitive inhibition by a structural analogue of the substrate (aminophenylethyl diphosphate) provided evidence for a mechanism of uptake by facilitated diffusion. The carrier identified may thus play a major role in supplying the plastid compartment with isopentenyl diphosphate for isoprenoid biosynthesis.
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Abbreviations
- APP:
-
aminophenylethyl diphosphate
- DMAPP:
-
dimethylallyl diphosphate
- GPP:
-
geranyl diphosphate
- IPP:
-
isopentenyl diphosphate
- NEM:
-
N-ethylmaleïmide
- PCMB:
-
p-chloromercuribenzoate
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Soler, E., Clastre, M., Bantignies, B. et al. Uptake of isopentenyl diphosphate by plastids isolated from Vitis vinifera L. cell suspensions. Planta 191, 324–329 (1993). https://doi.org/10.1007/BF00195689
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DOI: https://doi.org/10.1007/BF00195689