Summary
The human T-cell receptor gamma gene region spans 160 kb genomic DNA. Restriction fragment length polymorphisms (RFLPs) have been previously documented for the constant region (TRGC) genes, the joining (TRGJ) segments and the variable (TRGV) genes. We have recently defined the alleles of the T-cell receptor gamma V, J and C genes and we have described seven haplotypes of the V gamma subgroup I genes characterized either by RFLPs or by deletion or insertion of V gamma genes. The number of VγI genes may vary from 7 to 10 per haploid genome, the 9-gene haplotype being the most frequent. Allelic fragments can unambiguously characterize the TRGC2 gene with duplication or triplication of the exon 2. These alleles and haplotypes have been analyzed in four different populations (French, Lebanese, Tunisian and Black African). In this paper, we compare these allele and haplotype frequencies with those found in a Chinese population and we describe new TRGV allelic restriction fragments found only in the Chinese samples. These results and the previous data demonstrate the flexibility of the human T cell receptor gamma locus and the importance of unequal crossing-overs in the evolution of that locus. Moreover, they underline the importance of studying these polymorphisms in population genetics.
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Buresi C, Ghanem N, Huck S, Lefranc G, Lefranc M-P (1989) Exon duplication and triplication in the human T-cell receptor gamma constant region genes and RFLP in French, Lebanese, Tunisian and Black African populations. Immunogenetics 29:161–172
Font M-P, Chen Z, Bories JC, Duparc N, Loiseau P, Degos L, Cann H, Cohen D, Dausset J, Sigaux F (1988) The Vγ locus of the human T-cell receptor γ gene. Repertoire polymorphism of the first variable gene segment subgroup. J Exp Med 168:1383–1394
Forster A, Huck S, Ghanem N, Lefranc M-P, Rabbitts TH (1987) New subgroups in the human T-cell rearranging V gamma gene locus. EMBO J 6:1945–1950
Ghanem N, Lefranc M-P, Lefranc G (1988) Definition of the RFLP alleles in the human immunoglobulin IGHG locus. Eur J Immunol 18:1059–1065
Ghanem N, Buresi C, Lefranc G, Lefranc M-P (1989a) A unique RFLP in the human T-cell receptor gamma constant region gene TRGC1. Nucleic Acids Res 17:471
Ghanem N, Buresi C, Moisan JP, Bensmana M, Chuchana P, Huck S, Lefranc G, Lefranc M-P (1989b) Deletion, insertion and restriction site polymorphism of the T-cell receptor gamma variable locus in French, Lebanese, Tunisian and Black African populations. Immunogenetics 30:350–360
Ghanem N, Chuchana P, Dariavach P, Brockly F, Lefranc G, Lefranc M-P (1989c) SacI polymorphism defined by human T-cell receptor gamma (TRG) variable gene V11. Nucleic Acids Res 17:1271
Ghanem N, Dariavach P, Lefranc G, Lefranc M-P (1989d) A TaqI RFLP of the human T-cell receptor gamma (TRG) variable gene V11. Nucleic Acids Res 17:1270
Hochstenbach F, Parker C, McLean J, Gieselmann V, Band H, Bank I, Chess L, Spits H, Strominger JL, Seidman JG, Brenner MB (1988) Characterization of a third form of the human T-cell receptor γ/δ. J Exp Med 168:761–776
Huck S, Lefranc M-P (1987) Rearrangements to the JP1, JP and JP2 segments in the human T cell rearranging gamma gene (TRG) locus. FEBS Lett 224:291–296
Huck S, Dariavach P, Lefranc M-P (1988) Variable region genes in the human T cell rearranging gamma (TRG) locus: V-J junction and homology with the mouse genes. EMBO J 7:719–726
Lefranc M-P (1988) The human T-cell rearranging gamma (TRG) genes and the gamma T-cell receptor. Biochimie 70:901–908
Lefranc M-P, Rabbitts TH (1985) Two tandemly organized human genes encoding the T-cell gamma constant region sequences show multiple rearrangements in different T-cell types. Nature 316:464–466
Lefranc M-P, Rabbitts TH (1989) The human T-cell receptor γ (TRG) genes. Trends Biochem Sci 14:214–218
Lefranc M-P, Forster A, Rabbitts TH (1986a) Rearrangements of two distinct T-cell gamma-chain variable-region genes in human DNA. Nature 319:420–422
Lefranc M-P, Forster A, Rabbitts TH (1986b) Genetic polymorphism and exon changes of the constant regions of the human T-cell rearranging gene γ. Proc Natl Acad Sci USA 83:9596–9600
Lefranc M-P, Forster A, Baer R, Stinson MA, Rabbitts TH (1986c) Diversity and rearrangement of the human T-cell rearranging gamma genes: nine germ-line variable genes belonging to two subgroups. Cell 45:237–246
Lefranc M-P, Forster A, Rabbitts TH (1987) Organization of the human T-cell rearranging gamma genes (TRG). In: Kappler J, Davis M (eds) The T-cell receptor, vol 73. Liss, New York, pp 25–29
Lefranc M-P, Chuchana P, Dariavach P, Nguyen C, Huck S, Brockly F, Jordan B, Lefranc G (1989) Molecular mapping of the human T-cell receptor gamma (TRG) genes and linkage of the variable and constant regions. Eur J Immunol 19:989–994
Li Y, Szabo P, Posnett DN (1988) Molecular genotypes of the human T-cell receptor γ-chain. J Immunol 140:1300–1303
Littman DR, Newton M, Crommie D, Ang S-L, Seidman JG, Gettner SN, Weiss A (1987) Characterization of an expressed CD3- associated Ti γ-chain reveals C domain polymorphism. Nature 326:85–88
Pelicci PG, Subar M, Weiss A, Dalla-Favera R, Littman DR (1987) Molecular diversity of the human T-gamma constant region genes. Science 237:1051–1055
Quertermous T, Strauss WM, Dongen JJM van, Seidman JG (1987) Human T-cell γ-chain joining regions and T-cell development. J Immunol 138:2687–2690
Tighe L, Forster A, Clark D, Boylston A, Lavenir I, Rabbitts TH (1987) Unusual forms of T cell γ mRNA in a human T cell leukemia cell line: implications for the γ gene expression. Eur J Immunol 17:1729–1736
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Ghanem, N., Soua, Z., Zhang, X.G. et al. Polymorphism of the T-cell receptor gamma variable and constant region genes in a Chinese population. Hum Genet 86, 450–456 (1991). https://doi.org/10.1007/BF00194632
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DOI: https://doi.org/10.1007/BF00194632