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Rapid micropropagation for Mucuna pruriens f. pruriens L.

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Abstract

A rapid micropropagation system was developed for Mucuna pruriens f. pruriens using explants from 1-week-old aseptically grown seedlings. Multiple shoot regeneration occurred following an initial callus growth on Revised Tobacco (RT) medium supplemented with 2.7 μM NAA and 9.8 μM 2iP. Maximum number of shoot regeneration was achieved only from seedling explant 6 to 7 days old. More than 90% of the regenerated shoots could be rooted on half-strength liquid RT medium supplemented with 2.7 μM NAA. Plantlets readily adopted to greenhouse conditions. This system provides a new tool for micropropagation of Mucuna pruriens f. pruriens, an important medicinal plant.

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Abbreviations

BAP:

6-benzylaminopurine

Kn:

kinetin

2iP:

2-isopentenyladenine

AdS:

adenine sulphate

NAA:

alpha-naphthalene acetic acid

IAA:

indole-3-acetic acid

IBA:

indole-3-butyric acid

MS:

Murashige and Skoog (1962) medium

RT:

Revised Tobacco medium (Kaul and Staba, 1968)

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Authors and Affiliations

  1. Department of Botany, Visva Bharati University, 731235, Santiniketan, India

    Sharmila Chattopadhyay & S. K. Datta

  2. Indian Institute of Chemical Biology, 4, Raja S.C. Mullick Road, 700032, Calcutta, India

    S. B. Mahato

Authors
  1. Sharmila Chattopadhyay
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  2. S. K. Datta
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  3. S. B. Mahato
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Additional information

Communicated by W. Parrott

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Chattopadhyay, S., Datta, S.K. & Mahato, S.B. Rapid micropropagation for Mucuna pruriens f. pruriens L.. Plant Cell Reports 15, 271–273 (1995). https://doi.org/10.1007/BF00193734

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  • DOI: https://doi.org/10.1007/BF00193734

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