Summary
Comparison of different post-plating temperature regimes with a control treatment (27° C) revealed that a short-term cold (8/14°C:2/2 days or 14°C:4 days) as well as a heat treatment (30°C:14 days) increased the production of embryro-like-structures (ELS) from cultured maize anthers. The beneficial effects of short-term cold treatments were magnified 2–3 times when L-proline (PROL) was added to the induction medium (125–500 mg/L). In the best treatment (14°C:4 days, 125 mg/L L-proline) one genotype produced 143.5 ELS/100 anthers. Anthers subjected to high temperature (30°C:4 days, 30°C:7 days, 30°C:14 days) generally showed a lower response than did cold treated anthers, although genotypic differences were observed. Regeneration frequency did not appear to be affected by the presence of L-proline in the induction medium.
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Abbreviations
- ELS:
-
Embryo-like-structures
- PROL:
-
L-proline
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Communicated by A. R. Gould
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Büter, B., Schmid, J.E. & Stamp, P. Effects of L-proline and post-plating temperature treatment on Maize (Zea mays L.) anther culture. Plant Cell Reports 10, 325–328 (1991). https://doi.org/10.1007/BF00193151
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DOI: https://doi.org/10.1007/BF00193151