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Characterization of monoclonal antibodies to woodderived β-1,4-xylanase of Postia placenta and their application to detection of incipient decay

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Summary

Murine monoclonal antibodies (mAbs) were produced to the extracellular β-1,4-xy-lanase fraction of the brown-rot fungus, Postia placenta. The immunizing antigen was derived from decayed southern yellow pine wood blocks extracted in phosphate-buffered saline and Triton X-100, and fractionated by column chromatography. Five IgG monoclonal antibodies were selected for characterization by enzyme-linked immunosorbent assay, (ELISA) and western blot. Each mAb exhibited binding to two major protein bands on western blot at 32 kDa and 36 kDa. Characterization by chemical and enzymatic modification of the antigen determined whether mAbs were protein specific or specific for the carbohydrate portion of the glycoprotein. Affinity chromatography with the protein specific mAbs demonstrated specific binding to β-1,4-xylanase and cross-reactivity with β-1,3-glucanase, β-1,4-glucanase, and β-1,4-mannanase. These mAbs were also immunoreactive with polysaccharidases from liquid culture filtrates when tested by ELISA.The mAbs have been utilized to successfully detected P. placenta by ELISA in decayed southern yellow pine at a weight loss of 3.6%.

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Authors and Affiliations

  1. U.S. Department of Agriculture, Forest Service Forest Products Laboratory, Madison, Wisconsin

    Carol A. Clausen (Microbiologist), Frederick Green III (Microbiologist) & Terry L. Highley (Supervisory Research Plant Pathologist)

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  1. Carol A. Clausen
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  2. Frederick Green III
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  3. Terry L. Highley
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This article was written and prepared by U.S. Government employees on official time, and it is therefore the public domain and not subject to copyright. The Forest Products Laboratory is maintained in cooperation with the University of Wisconsin

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Clausen, C.A., Green, F. & Highley, T.L. Characterization of monoclonal antibodies to woodderived β-1,4-xylanase of Postia placenta and their application to detection of incipient decay. Wood Sci.Technol. 27, 219–228 (1993). https://doi.org/10.1007/BF00192818

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  • DOI: https://doi.org/10.1007/BF00192818

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