Abstract
We have studied the mechanism of Ca current inactivation in the β-cell line HIT-T15 by conventional and perforated patch recording techniques, using two pulse voltage protocols and a combination of current and tail current measurements. In 5 mM Ca, from a holding potential of - 80 mV, the maximum current showed a complex time course of inactivation: a relatively fast, double exponential inactivation (τh1 ≈ 12 ms and τh2 ≈ 60 ms) and a very slowly inactivating component (τ > 1 s). The faster component (τh1) was due to the voltage-dependent inactivation of a low-threshold-activated (LVA), T-type current, which deactivates more slowly (τ ≈ 3–5 ms) than the other components (τ ≈ 0.2–0.3 ms). The intermediate component (τh2) was due to the Ca-dependent inactivation of a portion of the high-threshold-activated (HVA) current. A saturating dose of the dihydropyridine (DHP) nifedipine (10 μM) did not affect the LVA current, but inhibited by 68 ± 5% the transient, Ca-sensitive portion of the HVA current and by 33 ± 12% the long lasting component. We suggest that three components of the calcium current can be resolved in HIT cells and the main target of DHPs is a HVA current, which inactivates faster than the DHP-resistant HVA component and does so primarily through calcium influx.
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Correspondence to: C. Marchetti
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Marchetti, C., Amico, C., Podestà, D. et al. Inactivation of voltage-dependent calcium current in an insulinoma cell line. Eur Biophys J 23, 51–58 (1994). https://doi.org/10.1007/BF00192205
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DOI: https://doi.org/10.1007/BF00192205